Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats

The present study aims to evaluate the hepatoprotective activity of stem aqueous extract of Caralluma europaea (AECe) on carbon tetrachloride- (CCl4-) induced hepatic damage in Wistar rats. The animals were daily treated with the aqueous extract of C. europaea at a dose of 250 mg/kg body weight for...

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Main Authors: Hayat Ouassou, Mohamed Bouhrim, Nour Elhouda Daoudi, Hassane Mekhfi, Abderrahim Ziyyat, Abdelkhaleq Legssyer, Mohamed Aziz, Mohamed Bnouham
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Advances in Pharmacological and Pharmaceutical Sciences
Online Access:http://dx.doi.org/10.1155/2021/8883040
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author Hayat Ouassou
Mohamed Bouhrim
Nour Elhouda Daoudi
Hassane Mekhfi
Abderrahim Ziyyat
Abdelkhaleq Legssyer
Mohamed Aziz
Mohamed Bnouham
author_facet Hayat Ouassou
Mohamed Bouhrim
Nour Elhouda Daoudi
Hassane Mekhfi
Abderrahim Ziyyat
Abdelkhaleq Legssyer
Mohamed Aziz
Mohamed Bnouham
author_sort Hayat Ouassou
collection DOAJ
description The present study aims to evaluate the hepatoprotective activity of stem aqueous extract of Caralluma europaea (AECe) on carbon tetrachloride- (CCl4-) induced hepatic damage in Wistar rats. The animals were daily treated with the aqueous extract of C. europaea at a dose of 250 mg/kg body weight for 14 days. CCl4 was injected (1 ml/kg, i.p.) two times, on the 7th and 14th days. At the end of the experimental period, all rats were anesthetized to collect blood for the assessment of biochemical parameters and then sacrificed to collect the liver for weighing. Hepatotoxicity was evaluated by measuring the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin (total and direct), malondialdehyde (MDA), total protein (TP), triglycerides (TG), total cholesterol, very low-density lipoprotein (VLDL-c ), low-density lipoprotein (LDL-c), high-density lipoprotein (HDL-c), urea, creatinine, and uric acid. Based on the results obtained in this study, the administration of C. europaea before exposure to the administration of CCl4 conferred favorable hepatoprotective effect in rats. The treatment with AECe (250 mg/kg) exhibits a significant hepatoprotective effect by ameliorating CCl4-induced alterations of these biochemical parameters. Hence, C. europaea could be a potential medicinal herb that can be used in the future to prevent liver intoxication.
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spelling doaj-art-792099b94d804e22b592d2bcd8b66bc42025-08-20T02:07:31ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902021-01-01202110.1155/2021/88830408883040Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar RatsHayat Ouassou0Mohamed Bouhrim1Nour Elhouda Daoudi2Hassane Mekhfi3Abderrahim Ziyyat4Abdelkhaleq Legssyer5Mohamed Aziz6Mohamed Bnouham7Laboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources Biotechnology Ethnopharmacology and Health, Mohammed First University, Oujda, MoroccoThe present study aims to evaluate the hepatoprotective activity of stem aqueous extract of Caralluma europaea (AECe) on carbon tetrachloride- (CCl4-) induced hepatic damage in Wistar rats. The animals were daily treated with the aqueous extract of C. europaea at a dose of 250 mg/kg body weight for 14 days. CCl4 was injected (1 ml/kg, i.p.) two times, on the 7th and 14th days. At the end of the experimental period, all rats were anesthetized to collect blood for the assessment of biochemical parameters and then sacrificed to collect the liver for weighing. Hepatotoxicity was evaluated by measuring the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin (total and direct), malondialdehyde (MDA), total protein (TP), triglycerides (TG), total cholesterol, very low-density lipoprotein (VLDL-c ), low-density lipoprotein (LDL-c), high-density lipoprotein (HDL-c), urea, creatinine, and uric acid. Based on the results obtained in this study, the administration of C. europaea before exposure to the administration of CCl4 conferred favorable hepatoprotective effect in rats. The treatment with AECe (250 mg/kg) exhibits a significant hepatoprotective effect by ameliorating CCl4-induced alterations of these biochemical parameters. Hence, C. europaea could be a potential medicinal herb that can be used in the future to prevent liver intoxication.http://dx.doi.org/10.1155/2021/8883040
spellingShingle Hayat Ouassou
Mohamed Bouhrim
Nour Elhouda Daoudi
Hassane Mekhfi
Abderrahim Ziyyat
Abdelkhaleq Legssyer
Mohamed Aziz
Mohamed Bnouham
Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
Advances in Pharmacological and Pharmaceutical Sciences
title Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
title_full Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
title_fullStr Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
title_full_unstemmed Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
title_short Evaluation of Hepatoprotective Activity of Caralluma europaea Stem Extract against CCl4-Induced Hepatic Damage in Wistar Rats
title_sort evaluation of hepatoprotective activity of caralluma europaea stem extract against ccl4 induced hepatic damage in wistar rats
url http://dx.doi.org/10.1155/2021/8883040
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