The effect of vitamin D deficiency on the RANKL/OPG ratio in rats

The aim of this study was to determine the effect of vitamin D deficiency on the RANKL/OPG ((Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin) ratio in the serum blood levels. Sixteen Wistar rats were divided into 2 groups: a control group (C) and an experimental group (E). The gr...

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Main Authors: Rawan M. Khalaf, Abdullazez A. Almudhi
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:Journal of Oral Biology and Craniofacial Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2212426822000100
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author Rawan M. Khalaf
Abdullazez A. Almudhi
author_facet Rawan M. Khalaf
Abdullazez A. Almudhi
author_sort Rawan M. Khalaf
collection DOAJ
description The aim of this study was to determine the effect of vitamin D deficiency on the RANKL/OPG ((Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin) ratio in the serum blood levels. Sixteen Wistar rats were divided into 2 groups: a control group (C) and an experimental group (E). The group C comprised of rats with average vitamin D levels, while vitamin D deficiency was induced in those of group E. A test period of 21 days was employed wherein two serum blood samples were obtained, at the baseline (day 0) and at day 21. Changes in RANKL andOPG levels were measured using Enzyme-linked immunosorbent assay (ELISA) kits. A statistically significant decrease of serum RANKL concentration and RANKL/OPG ratio on day 21 was seen in the experimental group compared to the control group. The serum RANKL levels and RANKL/OPG ratio in rats, were negatively affected by the deficiency of vitamin D.
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spelling doaj-art-79090485b58d43beaa5be5cd3dab6ea82025-08-20T02:50:29ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682022-03-0112222823210.1016/j.jobcr.2022.02.004The effect of vitamin D deficiency on the RANKL/OPG ratio in ratsRawan M. Khalaf0Abdullazez A. Almudhi1Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Graduate Studies and Scientific Research, P.O.Box 60169-15, Riyadh, 11545, Saudi Arabia; Corresponding author.Division of Orthodontics, Department of Pediatric Dentistry and Orthodontics, College of Dentistry, Graduate Studies and Scientific Research, P.O.Box 60169-15, Riyadh, 11545, Saudi ArabiaThe aim of this study was to determine the effect of vitamin D deficiency on the RANKL/OPG ((Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin) ratio in the serum blood levels. Sixteen Wistar rats were divided into 2 groups: a control group (C) and an experimental group (E). The group C comprised of rats with average vitamin D levels, while vitamin D deficiency was induced in those of group E. A test period of 21 days was employed wherein two serum blood samples were obtained, at the baseline (day 0) and at day 21. Changes in RANKL andOPG levels were measured using Enzyme-linked immunosorbent assay (ELISA) kits. A statistically significant decrease of serum RANKL concentration and RANKL/OPG ratio on day 21 was seen in the experimental group compared to the control group. The serum RANKL levels and RANKL/OPG ratio in rats, were negatively affected by the deficiency of vitamin D.http://www.sciencedirect.com/science/article/pii/S2212426822000100Vitamin D deficiencyRANKLOPGRANKL/OPG ratioPTHCalcium
spellingShingle Rawan M. Khalaf
Abdullazez A. Almudhi
The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
Journal of Oral Biology and Craniofacial Research
Vitamin D deficiency
RANKL
OPG
RANKL/OPG ratio
PTH
Calcium
title The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
title_full The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
title_fullStr The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
title_full_unstemmed The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
title_short The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
title_sort effect of vitamin d deficiency on the rankl opg ratio in rats
topic Vitamin D deficiency
RANKL
OPG
RANKL/OPG ratio
PTH
Calcium
url http://www.sciencedirect.com/science/article/pii/S2212426822000100
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