Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers

BackgroundThe World Health Organization highlighted the potential protective role of exercise against cognitive decline, all-cause dementia, Alzheimer’s disease (AD), and vascular dementia in healthy individuals. We have previously shown that exercise is particularly beneficial for older, cognitivel...

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Main Authors: Stephen M. Rao, Alexandria L. Chorba, M. J. Helppi, Alina Tuladhar, Amanda L. Penko, Sarah Holley, Brandon Nehls, Sabrina Paulino, Matthew C. Streicher, Audrey Zhu, Kellie Bruening, Sally Durgerian, Katherine Koenig, Mark Lowe, Wanyong Shin, Stephen E. Jones, Rachel Galioto, Jagan A. Pillai, Lynn M. Bekris, James B. Leverenz, Anson B. Rosenfeldt, Tamanna Singh, Gerald J. Beck, J. Carson Smith, Jay Alberts
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Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Aging Neuroscience
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Online Access:https://www.frontiersin.org/articles/10.3389/fnagi.2025.1584115/full
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author Stephen M. Rao
Alexandria L. Chorba
M. J. Helppi
Alina Tuladhar
Amanda L. Penko
Sarah Holley
Brandon Nehls
Sabrina Paulino
Matthew C. Streicher
Audrey Zhu
Kellie Bruening
Sally Durgerian
Katherine Koenig
Mark Lowe
Wanyong Shin
Stephen E. Jones
Rachel Galioto
Jagan A. Pillai
Lynn M. Bekris
James B. Leverenz
Anson B. Rosenfeldt
Tamanna Singh
Gerald J. Beck
J. Carson Smith
Jay Alberts
Jay Alberts
author_facet Stephen M. Rao
Alexandria L. Chorba
M. J. Helppi
Alina Tuladhar
Amanda L. Penko
Sarah Holley
Brandon Nehls
Sabrina Paulino
Matthew C. Streicher
Audrey Zhu
Kellie Bruening
Sally Durgerian
Katherine Koenig
Mark Lowe
Wanyong Shin
Stephen E. Jones
Rachel Galioto
Jagan A. Pillai
Lynn M. Bekris
James B. Leverenz
Anson B. Rosenfeldt
Tamanna Singh
Gerald J. Beck
J. Carson Smith
Jay Alberts
Jay Alberts
author_sort Stephen M. Rao
collection DOAJ
description BackgroundThe World Health Organization highlighted the potential protective role of exercise against cognitive decline, all-cause dementia, Alzheimer’s disease (AD), and vascular dementia in healthy individuals. We have previously shown that exercise is particularly beneficial for older, cognitively unimpaired apolipoprotein E4 (APOE ε4) carriers. A key unanswered question is whether a long-term, high-intensity aerobic exercise intervention initiated in a cohort of previously inactive older individuals at genetic risk for AD has neuroprotective properties.DesignCYCLE-AD is a randomized, single-blind, single-center, controlled trial of a home-based, high-intensity exercise intervention involving 150 older ε4 carriers (ages 65–80 years) who are healthy, cognitively unimpaired, and physically inactive. Participants are randomized into two groups: indoor cycling (IC) or usual and customary care (UCC) (target of 75 each). IC participants exercise 3×/week on an upright stationary cycle ergometer at a moderate-vigorous intensity for 18 months. Those in the UCC group are expected to maintain enrollment levels of activity.OutcomesComparison of IC and UCC groups on change in primary and secondary outcomes over baseline, 9-month, and 18-month evaluations. Primary outcomes are VO2peak (Fitness), 5-trial total recall on the Rey Auditory Verbal List Learning Test (Episodic Memory), and total hippocampal volume derived from structural MRI (Brain Atrophy). Secondary outcomes include comprehensive neurocognitive and physical function test batteries, MRI scans including structural and functional connectivity measures, and blood-based biomarkers.HypothesesOver an 18-month interval, physically inactive ε4 carriers who engage in high-intensity aerobic exercise will demonstrate less cognitive decline and hippocampal atrophy than physically inactive ε4 carriers who did not participate in a formal exercise program.ConclusionSuccessful demonstration of a scalable, home-based, high-intensity aerobic exercise intervention in altering the trajectory of AD pathophysiology and its effects on cognitive functioning will transform AD treatment, improve patient outcomes and quality of life, and reduce healthcare costs.
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spelling doaj-art-7906797e7fc844ceb7a8f23ffb82ce5a2025-08-20T03:12:32ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652025-05-011710.3389/fnagi.2025.15841151584115Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriersStephen M. Rao0Alexandria L. Chorba1M. J. Helppi2Alina Tuladhar3Amanda L. Penko4Sarah Holley5Brandon Nehls6Sabrina Paulino7Matthew C. Streicher8Audrey Zhu9Kellie Bruening10Sally Durgerian11Katherine Koenig12Mark Lowe13Wanyong Shin14Stephen E. Jones15Rachel Galioto16Jagan A. Pillai17Lynn M. Bekris18James B. Leverenz19Anson B. Rosenfeldt20Tamanna Singh21Gerald J. Beck22J. Carson Smith23Jay Alberts24Jay Alberts25Center for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesConcussion Center, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Quantitative Health Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Quantitative Health Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Diagnostic Radiology, Imaging Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Diagnostic Radiology, Imaging Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Diagnostic Radiology, Imaging Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Diagnostic Radiology, Imaging Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Diagnostic Radiology, Imaging Institute, Cleveland Clinic, Cleveland, OH, United StatesMellen Center, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesLerner Research Institute, Genomic Medicine Institute, Cleveland Clinic, Cleveland, OH, United StatesCenter for Brain Health, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Cardiovascular Medicine, Heart, Vascular and Thoracic Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Quantitative Health Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesDepartment of Kinesiology, School of Public Health, University of Maryland, College Park, MD, United StatesDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, United StatesConcussion Center, Neurological Institute, Cleveland Clinic, Cleveland, OH, United StatesBackgroundThe World Health Organization highlighted the potential protective role of exercise against cognitive decline, all-cause dementia, Alzheimer’s disease (AD), and vascular dementia in healthy individuals. We have previously shown that exercise is particularly beneficial for older, cognitively unimpaired apolipoprotein E4 (APOE ε4) carriers. A key unanswered question is whether a long-term, high-intensity aerobic exercise intervention initiated in a cohort of previously inactive older individuals at genetic risk for AD has neuroprotective properties.DesignCYCLE-AD is a randomized, single-blind, single-center, controlled trial of a home-based, high-intensity exercise intervention involving 150 older ε4 carriers (ages 65–80 years) who are healthy, cognitively unimpaired, and physically inactive. Participants are randomized into two groups: indoor cycling (IC) or usual and customary care (UCC) (target of 75 each). IC participants exercise 3×/week on an upright stationary cycle ergometer at a moderate-vigorous intensity for 18 months. Those in the UCC group are expected to maintain enrollment levels of activity.OutcomesComparison of IC and UCC groups on change in primary and secondary outcomes over baseline, 9-month, and 18-month evaluations. Primary outcomes are VO2peak (Fitness), 5-trial total recall on the Rey Auditory Verbal List Learning Test (Episodic Memory), and total hippocampal volume derived from structural MRI (Brain Atrophy). Secondary outcomes include comprehensive neurocognitive and physical function test batteries, MRI scans including structural and functional connectivity measures, and blood-based biomarkers.HypothesesOver an 18-month interval, physically inactive ε4 carriers who engage in high-intensity aerobic exercise will demonstrate less cognitive decline and hippocampal atrophy than physically inactive ε4 carriers who did not participate in a formal exercise program.ConclusionSuccessful demonstration of a scalable, home-based, high-intensity aerobic exercise intervention in altering the trajectory of AD pathophysiology and its effects on cognitive functioning will transform AD treatment, improve patient outcomes and quality of life, and reduce healthcare costs.https://www.frontiersin.org/articles/10.3389/fnagi.2025.1584115/fullAlzheimer’s diseaseexerciseneuroprotectionAPOE ε4randomized clinical trial
spellingShingle Stephen M. Rao
Alexandria L. Chorba
M. J. Helppi
Alina Tuladhar
Amanda L. Penko
Sarah Holley
Brandon Nehls
Sabrina Paulino
Matthew C. Streicher
Audrey Zhu
Kellie Bruening
Sally Durgerian
Katherine Koenig
Mark Lowe
Wanyong Shin
Stephen E. Jones
Rachel Galioto
Jagan A. Pillai
Lynn M. Bekris
James B. Leverenz
Anson B. Rosenfeldt
Tamanna Singh
Gerald J. Beck
J. Carson Smith
Jay Alberts
Jay Alberts
Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
Frontiers in Aging Neuroscience
Alzheimer’s disease
exercise
neuroprotection
APOE ε4
randomized clinical trial
title Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
title_full Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
title_fullStr Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
title_full_unstemmed Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
title_short Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers
title_sort protocol of a randomized single blind controlled trial of an 18 month home based high intensity exercise intervention in older cognitively unimpaired apoe ε4 carriers
topic Alzheimer’s disease
exercise
neuroprotection
APOE ε4
randomized clinical trial
url https://www.frontiersin.org/articles/10.3389/fnagi.2025.1584115/full
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