Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing
Salecan-based hydrogel (thereafter called Sal-hydrogel) dressings and gut microbiota have been associated with enhanced wound healing. However, the relationship between these two factors remains unclear. This study investigated the dynamic characteristics of the intestinal microbiota in relation to...
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| Format: | Article |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1584976/full |
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| author | Guangping Luo Guangping Luo Guangping Luo Tongtong Zhang Tongtong Zhang Tongtong Zhang Yanan Jiang Yanan Jiang Yuan Qin Yuan Qin Yuan Qin Pengfei Chen Junqing Hu Junqing Hu Junqing Hu |
| author_facet | Guangping Luo Guangping Luo Guangping Luo Tongtong Zhang Tongtong Zhang Tongtong Zhang Yanan Jiang Yanan Jiang Yuan Qin Yuan Qin Yuan Qin Pengfei Chen Junqing Hu Junqing Hu Junqing Hu |
| author_sort | Guangping Luo |
| collection | DOAJ |
| description | Salecan-based hydrogel (thereafter called Sal-hydrogel) dressings and gut microbiota have been associated with enhanced wound healing. However, the relationship between these two factors remains unclear. This study investigated the dynamic characteristics of the intestinal microbiota in relation to the Sal-hydrogel dressings and their role in promoting wound healing. Fecal samples were collected at day 0, 3, 7, and 12 after wounds were inflicted on 48 mice, which were treated with either Sal-hydrogel, Staphylococcus aureus, or a combination of S. aureus and Sal-hydrogel, using a full-thickness skin perforation wound model. The samples were subjected to 16S rRNA V3-V4 gene sequencing. The results indicated a general trend for Shannon diversity of the intestinal microbiota to increase by day 3 following injury. On the final day, the diversity in both the hydrogel group and the S. aureus plus hydrogel group was significantly higher compared to the control group (p < 0.05). Additionally, the bacterial community structure in the mouse gut exhibited substantial changes when comparing the hydrogel-treated groups to the controls (hydrogel group: p = 0.027; S. aureus plus hydrogel group: p = 0.039). The genus uncultured_Oscillospiraceae, which was significantly associated with wound closure (R-squared = 0.2154, p = 6.657e-05), was found to be dominant in the gut of the hydrogel group during the wound healing process. Overall, our findings suggest that significant and rapid alterations in gut microbiota occur in response to skin injury and wound infection. The enhanced wound healing properties of the Sal-hydrogel dressing are associated with increased intestinal microbiota diversity and the presence of the bacterium uncultured_Oscillospiraceae. |
| format | Article |
| id | doaj-art-78ee6f79dad84d1498e368f34066f6d2 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-78ee6f79dad84d1498e368f34066f6d22025-08-21T12:56:53ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-08-011310.3389/fbioe.2025.15849761584976Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressingGuangping Luo0Guangping Luo1Guangping Luo2Tongtong Zhang3Tongtong Zhang4Tongtong Zhang5Yanan Jiang6Yanan Jiang7Yuan Qin8Yuan Qin9Yuan Qin10Pengfei Chen11Junqing Hu12Junqing Hu13Junqing Hu14Obesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaThe Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaMedical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaObesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaThe Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaMedical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaObesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaThe Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaObesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaThe Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaMedical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaSchool of Food and Bioengineering, Xihua University, Chengdu, ChinaObesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaThe Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaMedical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, Chengdu, ChinaSalecan-based hydrogel (thereafter called Sal-hydrogel) dressings and gut microbiota have been associated with enhanced wound healing. However, the relationship between these two factors remains unclear. This study investigated the dynamic characteristics of the intestinal microbiota in relation to the Sal-hydrogel dressings and their role in promoting wound healing. Fecal samples were collected at day 0, 3, 7, and 12 after wounds were inflicted on 48 mice, which were treated with either Sal-hydrogel, Staphylococcus aureus, or a combination of S. aureus and Sal-hydrogel, using a full-thickness skin perforation wound model. The samples were subjected to 16S rRNA V3-V4 gene sequencing. The results indicated a general trend for Shannon diversity of the intestinal microbiota to increase by day 3 following injury. On the final day, the diversity in both the hydrogel group and the S. aureus plus hydrogel group was significantly higher compared to the control group (p < 0.05). Additionally, the bacterial community structure in the mouse gut exhibited substantial changes when comparing the hydrogel-treated groups to the controls (hydrogel group: p = 0.027; S. aureus plus hydrogel group: p = 0.039). The genus uncultured_Oscillospiraceae, which was significantly associated with wound closure (R-squared = 0.2154, p = 6.657e-05), was found to be dominant in the gut of the hydrogel group during the wound healing process. Overall, our findings suggest that significant and rapid alterations in gut microbiota occur in response to skin injury and wound infection. The enhanced wound healing properties of the Sal-hydrogel dressing are associated with increased intestinal microbiota diversity and the presence of the bacterium uncultured_Oscillospiraceae.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1584976/fullwound repairskin regenerationfaecal microbiotabacteriasalecan hydrogel |
| spellingShingle | Guangping Luo Guangping Luo Guangping Luo Tongtong Zhang Tongtong Zhang Tongtong Zhang Yanan Jiang Yanan Jiang Yuan Qin Yuan Qin Yuan Qin Pengfei Chen Junqing Hu Junqing Hu Junqing Hu Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing Frontiers in Bioengineering and Biotechnology wound repair skin regeneration faecal microbiota bacteria salecan hydrogel |
| title | Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| title_full | Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| title_fullStr | Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| title_full_unstemmed | Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| title_short | Dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| title_sort | dynamic male mouse gut microbiota signature linked to improved wound healing of a novel salecan hydrogel dressing |
| topic | wound repair skin regeneration faecal microbiota bacteria salecan hydrogel |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1584976/full |
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