Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages
Abstract This study synthesizes carbon dots derived from crude extracts of purple sweet potato (CPP-CDs) and evaluates its anti-inflammatory effects in a lipopolysaccharide (LPS) -induced acute inflammation model. Characterization revealed that CPP-CDs possess a uniform spherical structure and excel...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03494-9 |
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| author | Jiebang Jiang Zhiyuan Pan Yiren Su Lu Dai Nana Xu Han Wu Xin Chen |
| author_facet | Jiebang Jiang Zhiyuan Pan Yiren Su Lu Dai Nana Xu Han Wu Xin Chen |
| author_sort | Jiebang Jiang |
| collection | DOAJ |
| description | Abstract This study synthesizes carbon dots derived from crude extracts of purple sweet potato (CPP-CDs) and evaluates its anti-inflammatory effects in a lipopolysaccharide (LPS) -induced acute inflammation model. Characterization revealed that CPP-CDs possess a uniform spherical structure and excellent photoluminescent properties. In vitro, CPP-CDs significantly inhibited the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), reduced the accumulation of reactive oxygen species (ROS), suppressed pyroptosis, and facilitated the polarization of macrophages from the M1 phenotype to the M2 phenotype. In vivo, CPP-CDs significantly improved the survival rates of LPS-treated mice, mitigated tissue damage, and suppressed the levels of pro-inflammatory cytokines. Mechanistic studies indicated that CPP-CDs exert anti-inflammatory effects through the inhibition of the TLR4/NF-κB signaling pathway and the modulation of the NLRP3 inflammasome. Additionally, CPP-CDs exhibited excellent biocompatibility, with no significant toxicity observed in mice. This study provides strong evidence supporting the application of CPP-CDs as a novel anti-inflammatory material, highlighting their potential for acute inflammation treatment and expanding the possibilities for the development of carbon-dot-based anti-inflammatory therapies. |
| format | Article |
| id | doaj-art-78e34ac90fce4b47add8fc963947ab19 |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-78e34ac90fce4b47add8fc963947ab192025-08-20T02:00:01ZengBMCJournal of Nanobiotechnology1477-31552025-05-0123111710.1186/s12951-025-03494-9Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophagesJiebang Jiang0Zhiyuan Pan1Yiren Su2Lu Dai3Nana Xu4Han Wu5Xin Chen6Department of General Surgery, Affiliated Hospital of Nantong UniversitySchool of Life Sciences, Jiangsu Normal UniversitySchool of Life Sciences, Jiangsu Normal UniversityDepartment of General Surgery, Nantong Women’s Prison HospitalLaboratory of Morphology, Xuzhou Medical UniversityDepartment of General Surgery, Affiliated Hospital of Nantong UniversityDepartment of General Surgery, Affiliated Hospital of Nantong UniversityAbstract This study synthesizes carbon dots derived from crude extracts of purple sweet potato (CPP-CDs) and evaluates its anti-inflammatory effects in a lipopolysaccharide (LPS) -induced acute inflammation model. Characterization revealed that CPP-CDs possess a uniform spherical structure and excellent photoluminescent properties. In vitro, CPP-CDs significantly inhibited the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), reduced the accumulation of reactive oxygen species (ROS), suppressed pyroptosis, and facilitated the polarization of macrophages from the M1 phenotype to the M2 phenotype. In vivo, CPP-CDs significantly improved the survival rates of LPS-treated mice, mitigated tissue damage, and suppressed the levels of pro-inflammatory cytokines. Mechanistic studies indicated that CPP-CDs exert anti-inflammatory effects through the inhibition of the TLR4/NF-κB signaling pathway and the modulation of the NLRP3 inflammasome. Additionally, CPP-CDs exhibited excellent biocompatibility, with no significant toxicity observed in mice. This study provides strong evidence supporting the application of CPP-CDs as a novel anti-inflammatory material, highlighting their potential for acute inflammation treatment and expanding the possibilities for the development of carbon-dot-based anti-inflammatory therapies.https://doi.org/10.1186/s12951-025-03494-9Carbon dotsPurple sweet potatoReactive oxygen speciesTLR4/NF-κB signaling pathwayNLRP3 inflammasomeAnti-inflammatory material |
| spellingShingle | Jiebang Jiang Zhiyuan Pan Yiren Su Lu Dai Nana Xu Han Wu Xin Chen Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages Journal of Nanobiotechnology Carbon dots Purple sweet potato Reactive oxygen species TLR4/NF-κB signaling pathway NLRP3 inflammasome Anti-inflammatory material |
| title | Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages |
| title_full | Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages |
| title_fullStr | Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages |
| title_full_unstemmed | Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages |
| title_short | Carbon dots from purple sweet potato as a promising anti-inflammatory biomaterial for alleviating the LPS-induced inflammation in macrophages |
| title_sort | carbon dots from purple sweet potato as a promising anti inflammatory biomaterial for alleviating the lps induced inflammation in macrophages |
| topic | Carbon dots Purple sweet potato Reactive oxygen species TLR4/NF-κB signaling pathway NLRP3 inflammasome Anti-inflammatory material |
| url | https://doi.org/10.1186/s12951-025-03494-9 |
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