Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta

Abstract The circulatory system receives ionizing radiation at various dose rates. Given mounting epidemiological evidence of elevated radiation risks for diseases of the circulatory system (DCS), the International Commission on Radiological Protection recently recommended the first ever dose thresh...

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Main Authors: Nobuyuki Hamada, Ki-ichiro Kawano, Seiko Hirota, Farina Mohamad Yusoff, Takaharu Nomura, Yusuke Saito, Ayumu Nakashima, Shinji Yoshinaga, Yukihito Higashi
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-92710-5
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author Nobuyuki Hamada
Ki-ichiro Kawano
Seiko Hirota
Farina Mohamad Yusoff
Takaharu Nomura
Yusuke Saito
Ayumu Nakashima
Shinji Yoshinaga
Yukihito Higashi
author_facet Nobuyuki Hamada
Ki-ichiro Kawano
Seiko Hirota
Farina Mohamad Yusoff
Takaharu Nomura
Yusuke Saito
Ayumu Nakashima
Shinji Yoshinaga
Yukihito Higashi
author_sort Nobuyuki Hamada
collection DOAJ
description Abstract The circulatory system receives ionizing radiation at various dose rates. Given mounting epidemiological evidence of elevated radiation risks for diseases of the circulatory system (DCS), the International Commission on Radiological Protection recently recommended the first ever dose threshold for DCS. However, very little knowledge exists about whether radiation effects differ with dose rates and among tissues of the circulatory system. Here, we investigated the impact of dose rates in the carotid artery (CA) and compared it with the aorta. CA was obtained from mice irradiated with the same total dose that was delivered either acutely, 25 fractions, 100 fractions or chronically. CA underwent immunofluorescence and histochemistry staining. Irradiation led to vascular damage, inflammation and fibrosis in CA. The integrative analysis for 14 prelesional endpoints revealed that the magnitude of carotid changes was greater in 25 fractions, smaller in 100 fractions, and much smaller in chronic irradiation, compared with acute irradiation. Radiation responses of the aorta were qualitatively similar to, but quantitatively greater than those of CA. Irradiation causes sparing and enhancing dose protraction effects in a manner that is not a simple function of dose rate, and that radiosensitivity varies within the circulatory system.
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spelling doaj-art-78de8395d3ac4d4f8432dad046ed8c8f2025-08-20T01:57:44ZengNature PortfolioScientific Reports2045-23222025-03-0115111310.1038/s41598-025-92710-5Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aortaNobuyuki Hamada0Ki-ichiro Kawano1Seiko Hirota2Farina Mohamad Yusoff3Takaharu Nomura4Yusuke Saito5Ayumu Nakashima6Shinji Yoshinaga7Yukihito Higashi8Biology and Environmental Chemistry Division, Sustainable System Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI)Department of Regenerative Medicine, Division of Radiation Medical Science, Research Institute for Radiation Biology and Medicine, Hiroshima UniversityDepartment of Environmetrics and Biometrics, Division of Radiation Basic Science, Research Institute for Radiation Biology and Medicine, Hiroshima UniversityDepartment of Regenerative Medicine, Division of Radiation Medical Science, Research Institute for Radiation Biology and Medicine, Hiroshima UniversityBiology and Environmental Chemistry Division, Sustainable System Research Laboratory, Central Research Institute of Electric Power Industry (CRIEPI)Hiroshima University School of MedicineDepartment of Stem Cell Biology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Environmetrics and Biometrics, Division of Radiation Basic Science, Research Institute for Radiation Biology and Medicine, Hiroshima UniversityDepartment of Regenerative Medicine, Division of Radiation Medical Science, Research Institute for Radiation Biology and Medicine, Hiroshima UniversityAbstract The circulatory system receives ionizing radiation at various dose rates. Given mounting epidemiological evidence of elevated radiation risks for diseases of the circulatory system (DCS), the International Commission on Radiological Protection recently recommended the first ever dose threshold for DCS. However, very little knowledge exists about whether radiation effects differ with dose rates and among tissues of the circulatory system. Here, we investigated the impact of dose rates in the carotid artery (CA) and compared it with the aorta. CA was obtained from mice irradiated with the same total dose that was delivered either acutely, 25 fractions, 100 fractions or chronically. CA underwent immunofluorescence and histochemistry staining. Irradiation led to vascular damage, inflammation and fibrosis in CA. The integrative analysis for 14 prelesional endpoints revealed that the magnitude of carotid changes was greater in 25 fractions, smaller in 100 fractions, and much smaller in chronic irradiation, compared with acute irradiation. Radiation responses of the aorta were qualitatively similar to, but quantitatively greater than those of CA. Irradiation causes sparing and enhancing dose protraction effects in a manner that is not a simple function of dose rate, and that radiosensitivity varies within the circulatory system.https://doi.org/10.1038/s41598-025-92710-5Ionizing radiationDose rateFractionationCarotid arteryAorta
spellingShingle Nobuyuki Hamada
Ki-ichiro Kawano
Seiko Hirota
Farina Mohamad Yusoff
Takaharu Nomura
Yusuke Saito
Ayumu Nakashima
Shinji Yoshinaga
Yukihito Higashi
Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
Scientific Reports
Ionizing radiation
Dose rate
Fractionation
Carotid artery
Aorta
title Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
title_full Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
title_fullStr Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
title_full_unstemmed Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
title_short Responses of the carotid artery to acute, fractionated or chronic ionizing irradiation, and differences from the aorta
title_sort responses of the carotid artery to acute fractionated or chronic ionizing irradiation and differences from the aorta
topic Ionizing radiation
Dose rate
Fractionation
Carotid artery
Aorta
url https://doi.org/10.1038/s41598-025-92710-5
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