Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor
Paralichthys olivaceus P2X7 receptor (poP2X7R) is a recently identified as a P2X7 purinergic receptor involved in innate immunity of the Japanese flounder Paralichthys olivaceus. Divalent metals are allosteric modulators of mammalian P2XRs, but there is no information for fish P2XRs. Here, we charac...
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| Format: | Article |
| Language: | English |
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Wiley
2018-03-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12375 |
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| author | Carolina Paredes Shuo Li Xiaoli Chen Claudio Coddou |
| author_facet | Carolina Paredes Shuo Li Xiaoli Chen Claudio Coddou |
| author_sort | Carolina Paredes |
| collection | DOAJ |
| description | Paralichthys olivaceus P2X7 receptor (poP2X7R) is a recently identified as a P2X7 purinergic receptor involved in innate immunity of the Japanese flounder Paralichthys olivaceus. Divalent metals are allosteric modulators of mammalian P2XRs, but there is no information for fish P2XRs. Here, we characterized the effects of divalent metals on poP2X7R channel activity by electrophysiology and molecular biology techniques. Copper, zinc and mercury inhibited poP2X7R‐mediated currents with different maximal inhibition potency, while cadmium had no effect on poP2X7R activity. Mercury‐induced inhibition was irreversible, but the inhibitory effects of copper and zinc were reversed after washout. Cooper and zinc also reduced poP2X7R‐mediated interleukin‐1 mRNA production. These findings suggest that divalent metals have potential effects on the Japanese flounder innate immune response through modulation of poP2X7R activity. |
| format | Article |
| id | doaj-art-78d849bb18b44a9e9f4600fc6bd7e9e4 |
| institution | DOAJ |
| issn | 2211-5463 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-78d849bb18b44a9e9f4600fc6bd7e9e42025-08-20T02:39:15ZengWileyFEBS Open Bio2211-54632018-03-018338338910.1002/2211-5463.12375Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptorCarolina Paredes0Shuo Li1Xiaoli Chen2Claudio Coddou3Department of Biomedical Sciences Faculty of Medicine Universidad Católica del Norte Coquimbo ChileTianjin Key Laboratory of Animal and Plant Resistance College of Life Sciences Tianjin Normal University ChinaTianjin Key Laboratory of Animal and Plant Resistance College of Life Sciences Tianjin Normal University ChinaDepartment of Biomedical Sciences Faculty of Medicine Universidad Católica del Norte Coquimbo ChileParalichthys olivaceus P2X7 receptor (poP2X7R) is a recently identified as a P2X7 purinergic receptor involved in innate immunity of the Japanese flounder Paralichthys olivaceus. Divalent metals are allosteric modulators of mammalian P2XRs, but there is no information for fish P2XRs. Here, we characterized the effects of divalent metals on poP2X7R channel activity by electrophysiology and molecular biology techniques. Copper, zinc and mercury inhibited poP2X7R‐mediated currents with different maximal inhibition potency, while cadmium had no effect on poP2X7R activity. Mercury‐induced inhibition was irreversible, but the inhibitory effects of copper and zinc were reversed after washout. Cooper and zinc also reduced poP2X7R‐mediated interleukin‐1 mRNA production. These findings suggest that divalent metals have potential effects on the Japanese flounder innate immune response through modulation of poP2X7R activity.https://doi.org/10.1002/2211-5463.12375ATPdivalent metalsJapanese flounderParalichthys olivaceuspurinergic receptor P2X7 |
| spellingShingle | Carolina Paredes Shuo Li Xiaoli Chen Claudio Coddou Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor FEBS Open Bio ATP divalent metals Japanese flounder Paralichthys olivaceus purinergic receptor P2X7 |
| title | Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor |
| title_full | Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor |
| title_fullStr | Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor |
| title_full_unstemmed | Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor |
| title_short | Divalent metal modulation of Japanese flounder (Paralichthys olivaceus) purinergic P2X7 receptor |
| title_sort | divalent metal modulation of japanese flounder paralichthys olivaceus purinergic p2x7 receptor |
| topic | ATP divalent metals Japanese flounder Paralichthys olivaceus purinergic receptor P2X7 |
| url | https://doi.org/10.1002/2211-5463.12375 |
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