A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site
Subcutaneous transplantation is garnering attention as a potential transplantation site for pluripotent stem cell–derived islet cells to address the shortage of pancreatic islet transplant donors. However, subcutaneous transplantation of cells presents challenges related to angiogenesis, which is ne...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-06-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897251342986 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850222273897693184 |
|---|---|
| author | Norio Emoto Takayuki Anazawa Kei Yamane Nanae Fujimoto Takaaki Murakami Hiroyuki Fujimoto Cui Jialin Satoshi Ishida Kouki Kurahashi Aya Izuwa Hang Su Kenta Inoguchi Seiichiro Tada Kazuyuki Nagai Etsuro Hatano |
| author_facet | Norio Emoto Takayuki Anazawa Kei Yamane Nanae Fujimoto Takaaki Murakami Hiroyuki Fujimoto Cui Jialin Satoshi Ishida Kouki Kurahashi Aya Izuwa Hang Su Kenta Inoguchi Seiichiro Tada Kazuyuki Nagai Etsuro Hatano |
| author_sort | Norio Emoto |
| collection | DOAJ |
| description | Subcutaneous transplantation is garnering attention as a potential transplantation site for pluripotent stem cell–derived islet cells to address the shortage of pancreatic islet transplant donors. However, subcutaneous transplantation of cells presents challenges related to angiogenesis, which is necessary for successful islet bioproduction. This study aimed to investigate a novel method for enhancing vascularization at the transplant site and thereby promote islet engraftment using a clinically available bioabsorbable medical device. A nonabsorbable device (agarose) or a bioabsorbable device (collagen–gelatin sheet [CGS]) loaded with basic fibroblast growth factor (bFGF) was implanted subcutaneously in C57BL/6 mice. There were two other groups of mice, one of which was implanted with CGS alone, which acted as a control, and another group that was implanted with bFGF-loaded agarose rods. Subsequently, 200 islets were transplanted into the subcutaneous pre-vascularized sites. An equivalent number of islets was also transplanted into the portal vein (IPTx) to compare transplantation efficacy. Vascularization of the graft site was evaluated before and after transplantation. bFGF significantly enhanced angiogenesis in the CGS mice. The normalization rate of blood glucose levels following islet transplantation in the bFGF-loaded CGS was group comparable to that in the bFGF-loaded agarose rod and IPTx groups. The presence of islets was confirmed using single-photon emission computed tomography (SPECT/CT), histological examination. Furthermore, it was noted that blood glucose levels rapidly increased after graft removal, showing that graft function was crucial to maintain normoglycemia. Importantly, the bFGF-loaded CGS showed a high rate of engraftment. This novel bioabsorbable medical device method exhibited remarkable efficacy in enhancing subcutaneous islet engraftment, potentially paving the way for a more straightforward and less invasive approach for islet cell transplantation in future clinical applications. |
| format | Article |
| id | doaj-art-78d7c831de1d47a49fb6abbafa185ae0 |
| institution | OA Journals |
| issn | 1555-3892 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj-art-78d7c831de1d47a49fb6abbafa185ae02025-08-20T02:06:23ZengSAGE PublishingCell Transplantation1555-38922025-06-013410.1177/09636897251342986A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation siteNorio Emoto0Takayuki Anazawa1Kei Yamane2Nanae Fujimoto3Takaaki Murakami4Hiroyuki Fujimoto5Cui Jialin6Satoshi Ishida7Kouki Kurahashi8Aya Izuwa9Hang Su10Kenta Inoguchi11Seiichiro Tada12Kazuyuki Nagai13Etsuro Hatano14Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanInstitute for Life and Medical Sciences, Department of Regeneration Science and Engineering, Kyoto University, Kyoto, JapanDepartment of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, JapanRadioisotope Research Center, Agency for Health, Safety and Environment, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanDivision of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, JapanSubcutaneous transplantation is garnering attention as a potential transplantation site for pluripotent stem cell–derived islet cells to address the shortage of pancreatic islet transplant donors. However, subcutaneous transplantation of cells presents challenges related to angiogenesis, which is necessary for successful islet bioproduction. This study aimed to investigate a novel method for enhancing vascularization at the transplant site and thereby promote islet engraftment using a clinically available bioabsorbable medical device. A nonabsorbable device (agarose) or a bioabsorbable device (collagen–gelatin sheet [CGS]) loaded with basic fibroblast growth factor (bFGF) was implanted subcutaneously in C57BL/6 mice. There were two other groups of mice, one of which was implanted with CGS alone, which acted as a control, and another group that was implanted with bFGF-loaded agarose rods. Subsequently, 200 islets were transplanted into the subcutaneous pre-vascularized sites. An equivalent number of islets was also transplanted into the portal vein (IPTx) to compare transplantation efficacy. Vascularization of the graft site was evaluated before and after transplantation. bFGF significantly enhanced angiogenesis in the CGS mice. The normalization rate of blood glucose levels following islet transplantation in the bFGF-loaded CGS was group comparable to that in the bFGF-loaded agarose rod and IPTx groups. The presence of islets was confirmed using single-photon emission computed tomography (SPECT/CT), histological examination. Furthermore, it was noted that blood glucose levels rapidly increased after graft removal, showing that graft function was crucial to maintain normoglycemia. Importantly, the bFGF-loaded CGS showed a high rate of engraftment. This novel bioabsorbable medical device method exhibited remarkable efficacy in enhancing subcutaneous islet engraftment, potentially paving the way for a more straightforward and less invasive approach for islet cell transplantation in future clinical applications.https://doi.org/10.1177/09636897251342986 |
| spellingShingle | Norio Emoto Takayuki Anazawa Kei Yamane Nanae Fujimoto Takaaki Murakami Hiroyuki Fujimoto Cui Jialin Satoshi Ishida Kouki Kurahashi Aya Izuwa Hang Su Kenta Inoguchi Seiichiro Tada Kazuyuki Nagai Etsuro Hatano A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site Cell Transplantation |
| title | A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| title_full | A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| title_fullStr | A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| title_full_unstemmed | A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| title_short | A novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| title_sort | novel subcutaneous islet transplantation method using a bioabsorbable medical device to facilitate the creation of a highly vascularized transplantation site |
| url | https://doi.org/10.1177/09636897251342986 |
| work_keys_str_mv | AT norioemoto anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT takayukianazawa anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT keiyamane anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT nanaefujimoto anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT takaakimurakami anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT hiroyukifujimoto anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT cuijialin anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT satoshiishida anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT koukikurahashi anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT ayaizuwa anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT hangsu anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT kentainoguchi anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT seiichirotada anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT kazuyukinagai anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT etsurohatano anovelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT norioemoto novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT takayukianazawa novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT keiyamane novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT nanaefujimoto novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT takaakimurakami novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT hiroyukifujimoto novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT cuijialin novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT satoshiishida novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT koukikurahashi novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT ayaizuwa novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT hangsu novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT kentainoguchi novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT seiichirotada novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT kazuyukinagai novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite AT etsurohatano novelsubcutaneousislettransplantationmethodusingabioabsorbablemedicaldevicetofacilitatethecreationofahighlyvascularizedtransplantationsite |