The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats
Testicular torsion is one of the urologic emergencies occurring frequently in neonatal and adolescent period. Testis is sensitive to ischemia-reperfusion injury, and, therefore, ischemia and consecutive reperfusion cause an enhanced formation of reactive oxygen species that result in testicular cell...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/489248 |
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| author | Seda Ozbal Bekir Ugur Ergur Guven Erbil Isıl Tekmen Alper Bagrıyanık Zahide Cavdar |
| author_facet | Seda Ozbal Bekir Ugur Ergur Guven Erbil Isıl Tekmen Alper Bagrıyanık Zahide Cavdar |
| author_sort | Seda Ozbal |
| collection | DOAJ |
| description | Testicular torsion is one of the urologic emergencies occurring frequently in neonatal and adolescent period. Testis is sensitive to ischemia-reperfusion injury, and, therefore, ischemia and consecutive reperfusion cause an enhanced formation of reactive oxygen species that result in testicular cell damage and apoptosis. α-lipoic acid is a free radical scavenger and a biological antioxidant. It is widely used in the prevention of oxidative stress and cellular damage. We aimed to investigate the protective effect of α-lipoic acid on testicular damage in rats subjected to testicular ischemia-reperfusion injury. 35 rats were randomly divided into 5 groups: control, sham operated, ischemia, ischemia-reperfusion, and ischemia-reperfusion +lipoic acid groups, 2 h torsion and 2 h detorsion of the testis were performed. Testicular cell damage was examined by H-E staining. TUNEL and active caspase-3 immunostaining were used to detect germ cell apoptosis. GPx , SOD activity, and MDA levels were evaluated. Histological evaluation showed that α-lipoic acid pretreatment reduced testicular cell damage and decreased TUNEL and caspase-3-positive cells. Additionally, α-lipoic acid administration decreased the GPx and SOD activity and increased the MDA levels. The present results suggest that LA is a potentially beneficial agent in protecting testicular I/R in rats. |
| format | Article |
| id | doaj-art-78d6639fc28f400c85636b7622186aad |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-78d6639fc28f400c85636b7622186aad2025-08-20T02:07:30ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/489248489248The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in RatsSeda Ozbal0Bekir Ugur Ergur1Guven Erbil2Isıl Tekmen3Alper Bagrıyanık4Zahide Cavdar5Department of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, TurkeyDepartment of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, TurkeyDepartment of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, TurkeyDepartment of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, TurkeyDepartment of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, TurkeyDepartment of Molecular Medicine, Health Science Institute, School of Medicine, Dokuz Eylül University, 35340 İzmir, TurkeyTesticular torsion is one of the urologic emergencies occurring frequently in neonatal and adolescent period. Testis is sensitive to ischemia-reperfusion injury, and, therefore, ischemia and consecutive reperfusion cause an enhanced formation of reactive oxygen species that result in testicular cell damage and apoptosis. α-lipoic acid is a free radical scavenger and a biological antioxidant. It is widely used in the prevention of oxidative stress and cellular damage. We aimed to investigate the protective effect of α-lipoic acid on testicular damage in rats subjected to testicular ischemia-reperfusion injury. 35 rats were randomly divided into 5 groups: control, sham operated, ischemia, ischemia-reperfusion, and ischemia-reperfusion +lipoic acid groups, 2 h torsion and 2 h detorsion of the testis were performed. Testicular cell damage was examined by H-E staining. TUNEL and active caspase-3 immunostaining were used to detect germ cell apoptosis. GPx , SOD activity, and MDA levels were evaluated. Histological evaluation showed that α-lipoic acid pretreatment reduced testicular cell damage and decreased TUNEL and caspase-3-positive cells. Additionally, α-lipoic acid administration decreased the GPx and SOD activity and increased the MDA levels. The present results suggest that LA is a potentially beneficial agent in protecting testicular I/R in rats.http://dx.doi.org/10.1100/2012/489248 |
| spellingShingle | Seda Ozbal Bekir Ugur Ergur Guven Erbil Isıl Tekmen Alper Bagrıyanık Zahide Cavdar The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats The Scientific World Journal |
| title | The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats |
| title_full | The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats |
| title_fullStr | The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats |
| title_full_unstemmed | The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats |
| title_short | The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats |
| title_sort | effects of α lipoic acid against testicular ischemia reperfusion injury in rats |
| url | http://dx.doi.org/10.1100/2012/489248 |
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