Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications

Optical coherence tomography (OCT) phantoms are essential tools for calibrating imaging systems, validating diagnostic algorithms, and bridging technological advancements with clinical applications. This review explores the development and application of materials used in OCT phantoms, emphasising t...

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Main Author: Mukhit Kulmaganbetov
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:BioChem
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Online Access:https://www.mdpi.com/2673-6411/5/2/6
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author Mukhit Kulmaganbetov
author_facet Mukhit Kulmaganbetov
author_sort Mukhit Kulmaganbetov
collection DOAJ
description Optical coherence tomography (OCT) phantoms are essential tools for calibrating imaging systems, validating diagnostic algorithms, and bridging technological advancements with clinical applications. This review explores the development and application of materials used in OCT phantoms, emphasising their optical, mechanical, and biochemical fidelity to biological tissues. Gelatin-based phantoms (<i>n</i> = 1.35) offer controllable absorbance and scattering, with penetration depths (PDs) of 500–2000 µm and scattering coefficients (SCs) of 5–20 cm<sup>−1</sup> but are unstable at room temperature. Silicone phantoms (<i>n</i> = 1.41) are durable and stable, with SCs of 10–15 cm<sup>−1</sup>, suitable for long-term studies. Polydimethylsiloxane (PDMS) phantoms (<i>n</i> = 1.41) provide manageable optical properties and are used in microfluidic applications. Polyvinyl alcohol (PVA) phantoms (<i>n</i> = 1.48) mimic soft tissue mechanics, with SCs of 5–15 cm<sup>−1</sup>, but require freeze–thaw cycles. Fibrin phantoms (<i>n</i> = 1.38) simulate blood clotting, with SCs of 5–20 cm<sup>−1</sup>. Scattering particles like polystyrene (<i>n</i> = 1.57) and titanium dioxide (TiO<sub>2</sub>, <i>n</i> = 2.49) offer modifiable properties, while silica microspheres (SiO<sub>2</sub>, <i>n</i> = 3.6) and gold nanoshells (<i>n</i> = 2.59) provide customisable optical characteristics. These materials and particles are crucial for simulating biological tissues, enhancing OCT imaging, and developing diagnostic applications. Despite progress, challenges persist in achieving submicron resolution, long-term stability, and cost-effective scalability.
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spelling doaj-art-78c67ff40ad1426e82f4206cdf85229b2025-08-20T03:32:31ZengMDPI AGBioChem2673-64112025-04-0152610.3390/biochem5020006Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and ApplicationsMukhit Kulmaganbetov0Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong KongOptical coherence tomography (OCT) phantoms are essential tools for calibrating imaging systems, validating diagnostic algorithms, and bridging technological advancements with clinical applications. This review explores the development and application of materials used in OCT phantoms, emphasising their optical, mechanical, and biochemical fidelity to biological tissues. Gelatin-based phantoms (<i>n</i> = 1.35) offer controllable absorbance and scattering, with penetration depths (PDs) of 500–2000 µm and scattering coefficients (SCs) of 5–20 cm<sup>−1</sup> but are unstable at room temperature. Silicone phantoms (<i>n</i> = 1.41) are durable and stable, with SCs of 10–15 cm<sup>−1</sup>, suitable for long-term studies. Polydimethylsiloxane (PDMS) phantoms (<i>n</i> = 1.41) provide manageable optical properties and are used in microfluidic applications. Polyvinyl alcohol (PVA) phantoms (<i>n</i> = 1.48) mimic soft tissue mechanics, with SCs of 5–15 cm<sup>−1</sup>, but require freeze–thaw cycles. Fibrin phantoms (<i>n</i> = 1.38) simulate blood clotting, with SCs of 5–20 cm<sup>−1</sup>. Scattering particles like polystyrene (<i>n</i> = 1.57) and titanium dioxide (TiO<sub>2</sub>, <i>n</i> = 2.49) offer modifiable properties, while silica microspheres (SiO<sub>2</sub>, <i>n</i> = 3.6) and gold nanoshells (<i>n</i> = 2.59) provide customisable optical characteristics. These materials and particles are crucial for simulating biological tissues, enhancing OCT imaging, and developing diagnostic applications. Despite progress, challenges persist in achieving submicron resolution, long-term stability, and cost-effective scalability.https://www.mdpi.com/2673-6411/5/2/6optical coherence tomographyphantomscattering
spellingShingle Mukhit Kulmaganbetov
Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
BioChem
optical coherence tomography
phantom
scattering
title Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
title_full Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
title_fullStr Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
title_full_unstemmed Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
title_short Advancements in Retinal Tissue-Mimicking Optical Coherence Tomography Phantoms: Materials, Properties, and Applications
title_sort advancements in retinal tissue mimicking optical coherence tomography phantoms materials properties and applications
topic optical coherence tomography
phantom
scattering
url https://www.mdpi.com/2673-6411/5/2/6
work_keys_str_mv AT mukhitkulmaganbetov advancementsinretinaltissuemimickingopticalcoherencetomographyphantomsmaterialspropertiesandapplications