Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts
In tumour cell lines, the resistance of cancer cells to a variety of structurally unrelated chemotherapeutic drugs is termed multidrug‐resistance or MDR. We reported previously that MDR leukemic cells displayed nuclear texture changes, as assessed by image cytometry. The nature of these changes rema...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1999/967803 |
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| author | Naima Rafki‐Beljebbar Françoise Liautaud‐Roger Dominique Ploton Jean Dufer |
| author_facet | Naima Rafki‐Beljebbar Françoise Liautaud‐Roger Dominique Ploton Jean Dufer |
| author_sort | Naima Rafki‐Beljebbar |
| collection | DOAJ |
| description | In tumour cell lines, the resistance of cancer cells to a variety of structurally unrelated chemotherapeutic drugs is termed multidrug‐resistance or MDR. We reported previously that MDR leukemic cells displayed nuclear texture changes, as assessed by image cytometry. The nature of these changes remained uncertain but they could be associated with alterations of the nuclear matrix which could serve an important role in DNA organization and chromatin structure. Therefore, we have compared the textural features observed in G0/G1 nuclei from human leukemic CEM cells and their MDR variant CEM‐VLB, after staining of either DNA by Feulgen method or nuclear matrix by immunodetection of NuMA antigen on DNase treated samples. Chromatin or NuMA distributions within the nucleus were evaluated by image cytometry. Changes in textural parameters indicate that modifications of NuMA distribution observed in MDR cells are parallel to those observed at the whole chromatin level (i.e., a more decondensed and coarse texture with increase of Energy and Long‐run sections and decrease of Contrast and Short‐run sections). Moreover, Optical Densities measurements indicate that MDR cells seem to contain less NuMA, a datum confirmed by immunoblotting of nuclear proteins. In conclusion, chromatin changes observed by image cytometry in drug‐resistant human leukemic CEM cells appear associated with modifications of the nuclear matrix structure. |
| format | Article |
| id | doaj-art-78c3a113f8594ac4883bf7305e9c0817 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-78c3a113f8594ac4883bf7305e9c08172025-02-03T01:00:53ZengWileyAnalytical Cellular Pathology0921-89121878-36511999-01-0118417518110.1155/1999/967803Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM LymphoblastsNaima Rafki‐Beljebbar0Françoise Liautaud‐Roger1Dominique Ploton2Jean Dufer3Unité MéDIAN, EA2063, IFR 53, Faculté de Pharmacie, Université de Reims, 51 rue Cognacq‐Jay, Reims, FranceInstitut Jean‐Godinot, 1, rue Gal Koenig, Reims, FranceUnité MéDIAN, EA2063, IFR 53, Faculté de Pharmacie, Université de Reims, 51 rue Cognacq‐Jay, Reims, FranceUnité MéDIAN, EA2063, IFR 53, Faculté de Pharmacie, Université de Reims, 51 rue Cognacq‐Jay, Reims, FranceIn tumour cell lines, the resistance of cancer cells to a variety of structurally unrelated chemotherapeutic drugs is termed multidrug‐resistance or MDR. We reported previously that MDR leukemic cells displayed nuclear texture changes, as assessed by image cytometry. The nature of these changes remained uncertain but they could be associated with alterations of the nuclear matrix which could serve an important role in DNA organization and chromatin structure. Therefore, we have compared the textural features observed in G0/G1 nuclei from human leukemic CEM cells and their MDR variant CEM‐VLB, after staining of either DNA by Feulgen method or nuclear matrix by immunodetection of NuMA antigen on DNase treated samples. Chromatin or NuMA distributions within the nucleus were evaluated by image cytometry. Changes in textural parameters indicate that modifications of NuMA distribution observed in MDR cells are parallel to those observed at the whole chromatin level (i.e., a more decondensed and coarse texture with increase of Energy and Long‐run sections and decrease of Contrast and Short‐run sections). Moreover, Optical Densities measurements indicate that MDR cells seem to contain less NuMA, a datum confirmed by immunoblotting of nuclear proteins. In conclusion, chromatin changes observed by image cytometry in drug‐resistant human leukemic CEM cells appear associated with modifications of the nuclear matrix structure.http://dx.doi.org/10.1155/1999/967803 |
| spellingShingle | Naima Rafki‐Beljebbar Françoise Liautaud‐Roger Dominique Ploton Jean Dufer Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts Analytical Cellular Pathology |
| title | Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts |
| title_full | Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts |
| title_fullStr | Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts |
| title_full_unstemmed | Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts |
| title_short | Textural Analysis of Nuclear Mitotic Apparatus Antigen (NuMA) Spatial Distribution in Interphase Nuclei from Human Drug-Resistant CEM Lymphoblasts |
| title_sort | textural analysis of nuclear mitotic apparatus antigen numa spatial distribution in interphase nuclei from human drug resistant cem lymphoblasts |
| url | http://dx.doi.org/10.1155/1999/967803 |
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