Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes

This study evaluated the viability of using the solid residues (bagasse) of the mezcal industry produced with <i>Agave durangensis</i> as a substrate for biogas production, using two chemical pretreatments, acid (HCl) and alkaline (KOH + Ca(OH)<sub>2</sub>), to enhance its bi...

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Main Authors: Refugio Hernández-López, Iván Moreno-Andrade, Blanca E. Barragán-Huerta, Edson B. Estrada-Arriaga, Marco A. Garzón-Zúñiga
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Fermentation
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Online Access:https://www.mdpi.com/2311-5637/11/7/399
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author Refugio Hernández-López
Iván Moreno-Andrade
Blanca E. Barragán-Huerta
Edson B. Estrada-Arriaga
Marco A. Garzón-Zúñiga
author_facet Refugio Hernández-López
Iván Moreno-Andrade
Blanca E. Barragán-Huerta
Edson B. Estrada-Arriaga
Marco A. Garzón-Zúñiga
author_sort Refugio Hernández-López
collection DOAJ
description This study evaluated the viability of using the solid residues (bagasse) of the mezcal industry produced with <i>Agave durangensis</i> as a substrate for biogas production, using two chemical pretreatments, acid (HCl) and alkaline (KOH + Ca(OH)<sub>2</sub>), to enhance its biodegradability and improve the anaerobic digestion (AD) process. The chemical composition of bagasse was analyzed before and after the chemical pretreatments and then AD experiments were conducted in anaerobic sequential batch reactors (A-SBR) to analyze the effect of pretreatments on biogas production performance. The results showed that acid pretreatment increased cellulose content to 0.606 g, which represented an increase of 34%, and significantly reduced hemicellulose. In contrast, alkaline pretreatment did not show significant changes in cellulose composition, although it caused a swelling of the <i>Agave durangensis</i> mezcal bagasse (Ad-MB) fibers. In terms of biogas production, Ad-MB pretreated with acid (Ad-MB-acid) increased cumulative production by 76% compared to the <i>Agave durangensis</i> mezcal bagasse that was not pretreated (Ad-MB-not pretreated) and by 135% compared to <i>Agave durangensis</i> mezcal bagasse pretreated with an alkaline solution (Ad-MB-alkaline). These results confirmed that <i>Agave durangensis</i> solid waste from the mezcal industry that receives acidic chemical pretreatment has the potential to generate biogas as a sustainable biofuel that can be used to reduce the ecological footprint of this industry.
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series Fermentation
spelling doaj-art-78bfa6c8b80d4b84b139e536a754d0f72025-08-20T03:07:58ZengMDPI AGFermentation2311-56372025-07-0111739910.3390/fermentation11070399Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical ProcessesRefugio Hernández-López0Iván Moreno-Andrade1Blanca E. Barragán-Huerta2Edson B. Estrada-Arriaga3Marco A. Garzón-Zúñiga4Laboratorio de Evaluación, Desarrollo e Innovación de Tecnología del Agua, CIIDIR-Durango, Instituto Politécnico Nacional, Sigma 119, 20 de Noviembre II, Durango 34220, MexicoLaboratory of Research on Advanced Processes for Water Treatment, Juriquilla Academic Unit, Institute of Engineering, National Autonomous University of Mexico, Blvd. Juriquilla 3001, Querétaro 76230, MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Unidad Profesional Adolfo López Mateos, Mexico City 07738, MexicoSubcoordinación de Sistemas de Saneamiento y Reutilización de Aguas Residuales, Instituto Mexicano de Tecnología del Agua, Paseo Cuauhnáhuac 8532, Progreso, Jiutepec 62550, MexicoLaboratorio de Evaluación, Desarrollo e Innovación de Tecnología del Agua, CIIDIR-Durango, Instituto Politécnico Nacional, Sigma 119, 20 de Noviembre II, Durango 34220, MexicoThis study evaluated the viability of using the solid residues (bagasse) of the mezcal industry produced with <i>Agave durangensis</i> as a substrate for biogas production, using two chemical pretreatments, acid (HCl) and alkaline (KOH + Ca(OH)<sub>2</sub>), to enhance its biodegradability and improve the anaerobic digestion (AD) process. The chemical composition of bagasse was analyzed before and after the chemical pretreatments and then AD experiments were conducted in anaerobic sequential batch reactors (A-SBR) to analyze the effect of pretreatments on biogas production performance. The results showed that acid pretreatment increased cellulose content to 0.606 g, which represented an increase of 34%, and significantly reduced hemicellulose. In contrast, alkaline pretreatment did not show significant changes in cellulose composition, although it caused a swelling of the <i>Agave durangensis</i> mezcal bagasse (Ad-MB) fibers. In terms of biogas production, Ad-MB pretreated with acid (Ad-MB-acid) increased cumulative production by 76% compared to the <i>Agave durangensis</i> mezcal bagasse that was not pretreated (Ad-MB-not pretreated) and by 135% compared to <i>Agave durangensis</i> mezcal bagasse pretreated with an alkaline solution (Ad-MB-alkaline). These results confirmed that <i>Agave durangensis</i> solid waste from the mezcal industry that receives acidic chemical pretreatment has the potential to generate biogas as a sustainable biofuel that can be used to reduce the ecological footprint of this industry.https://www.mdpi.com/2311-5637/11/7/399<i>Agave durangensis</i>anaerobic digestionbiogas productionchemical pretreatmentmezcalmezcal bagasse wastes
spellingShingle Refugio Hernández-López
Iván Moreno-Andrade
Blanca E. Barragán-Huerta
Edson B. Estrada-Arriaga
Marco A. Garzón-Zúñiga
Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
Fermentation
<i>Agave durangensis</i>
anaerobic digestion
biogas production
chemical pretreatment
mezcal
mezcal bagasse wastes
title Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
title_full Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
title_fullStr Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
title_full_unstemmed Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
title_short Biogas Production from <i>Agave durangensis</i> Mezcal Bagasse Pretreated Using Chemical Processes
title_sort biogas production from i agave durangensis i mezcal bagasse pretreated using chemical processes
topic <i>Agave durangensis</i>
anaerobic digestion
biogas production
chemical pretreatment
mezcal
mezcal bagasse wastes
url https://www.mdpi.com/2311-5637/11/7/399
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