Electrical stimulation induced pre-vascularization of engineered dental pulp tissue
Vascularization is a key step to achieve pulp tissue regeneration and in vitro pre-vascularized dental pulp tissue could be applied as a graft substitute for dental pulp tissue repair. In this study, human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (hUVECs) were co-cul...
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| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001214 |
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| author | Ying-tong Wang Jia-ying Zhou Kai Chen Xiao Yu Zhi-yong Dong Yu-shan Liu Xiao-ting Meng |
| author_facet | Ying-tong Wang Jia-ying Zhou Kai Chen Xiao Yu Zhi-yong Dong Yu-shan Liu Xiao-ting Meng |
| author_sort | Ying-tong Wang |
| collection | DOAJ |
| description | Vascularization is a key step to achieve pulp tissue regeneration and in vitro pre-vascularized dental pulp tissue could be applied as a graft substitute for dental pulp tissue repair. In this study, human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (hUVECs) were co-cultured in 3D Matrigel and 150 mV/mm electric fields (EFs) were used to promote the construction of pre-vascularized dental pulp tissue. After optimizing co-cultured ratio of two cell types, immunofluorescence staining, and live/dead detection were used to investigate the effect of EFs on cell survival, differentiation and vessel formation in 3D engineered dental pulp tissue. RNA sequencing was used to investigate the potential molecular mechanisms by which EF regulates vessel formation in 3D engineered dental pulp tissue. Here we identified that EF-induced pre-vascularized engineered dental pulp tissue not only had odontoblasts, but also had a rich vascular network, and smooth muscle-like cells appeared around the blood vessels. The GO enrichment analysis showed that these genes were significantly enriched in regulation of angiogenesis, cell migration and motility. The most significant term of the KEGG pathway analysis were NOTCH signaling pathway and Calcium signaling pathway etc. The PPI network revealed that NOTCH1 and IL-6 were central hub genes. Our study indicated that EFs significantly promoted the maturation and stable of blood vessel in 3D engineered pulp tissue and provided an experimental basis for the application of EF in dental pulp angiogenesis and regeneration. |
| format | Article |
| id | doaj-art-78aee00d4def42f29243398e7b5fd5a1 |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-78aee00d4def42f29243398e7b5fd5a12025-08-20T02:07:34ZengElsevierRegenerative Therapy2352-32042024-06-012635436510.1016/j.reth.2024.06.014Electrical stimulation induced pre-vascularization of engineered dental pulp tissueYing-tong Wang0Jia-ying Zhou1Kai Chen2Xiao Yu3Zhi-yong Dong4Yu-shan Liu5Xiao-ting Meng6Department of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, China; The Undergraduate Center of Hospital of Stomatology, Jilin University, Changchun 130021, ChinaDepartment of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaNorman Bethune Stomatological School of Jilin University, Changchun 130021, ChinaDepartment of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, ChinaDepartment of Histology & Embryology, College of Basic Medical Sciences, Jilin University, Changchun, China; Corresponding author.Vascularization is a key step to achieve pulp tissue regeneration and in vitro pre-vascularized dental pulp tissue could be applied as a graft substitute for dental pulp tissue repair. In this study, human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (hUVECs) were co-cultured in 3D Matrigel and 150 mV/mm electric fields (EFs) were used to promote the construction of pre-vascularized dental pulp tissue. After optimizing co-cultured ratio of two cell types, immunofluorescence staining, and live/dead detection were used to investigate the effect of EFs on cell survival, differentiation and vessel formation in 3D engineered dental pulp tissue. RNA sequencing was used to investigate the potential molecular mechanisms by which EF regulates vessel formation in 3D engineered dental pulp tissue. Here we identified that EF-induced pre-vascularized engineered dental pulp tissue not only had odontoblasts, but also had a rich vascular network, and smooth muscle-like cells appeared around the blood vessels. The GO enrichment analysis showed that these genes were significantly enriched in regulation of angiogenesis, cell migration and motility. The most significant term of the KEGG pathway analysis were NOTCH signaling pathway and Calcium signaling pathway etc. The PPI network revealed that NOTCH1 and IL-6 were central hub genes. Our study indicated that EFs significantly promoted the maturation and stable of blood vessel in 3D engineered pulp tissue and provided an experimental basis for the application of EF in dental pulp angiogenesis and regeneration.http://www.sciencedirect.com/science/article/pii/S2352320424001214Electric fieldPre-vascularized dental pulp tissueDental pulp stem cellsHuman umbilical vein endothelial cells |
| spellingShingle | Ying-tong Wang Jia-ying Zhou Kai Chen Xiao Yu Zhi-yong Dong Yu-shan Liu Xiao-ting Meng Electrical stimulation induced pre-vascularization of engineered dental pulp tissue Regenerative Therapy Electric field Pre-vascularized dental pulp tissue Dental pulp stem cells Human umbilical vein endothelial cells |
| title | Electrical stimulation induced pre-vascularization of engineered dental pulp tissue |
| title_full | Electrical stimulation induced pre-vascularization of engineered dental pulp tissue |
| title_fullStr | Electrical stimulation induced pre-vascularization of engineered dental pulp tissue |
| title_full_unstemmed | Electrical stimulation induced pre-vascularization of engineered dental pulp tissue |
| title_short | Electrical stimulation induced pre-vascularization of engineered dental pulp tissue |
| title_sort | electrical stimulation induced pre vascularization of engineered dental pulp tissue |
| topic | Electric field Pre-vascularized dental pulp tissue Dental pulp stem cells Human umbilical vein endothelial cells |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001214 |
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