Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like
The promoter plays an important role in the regulation of gene expression. The problem is some of binary vector that absence from promoter at cloning site. The cauliflower mosaic virus (CaMV) 35S promoter is a strong and constitutive promoter that widely used to produce transgenic organisms. In this...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2015-06-01
|
| Series: | Journal of Experimental Life Science |
| Subjects: | |
| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/152 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850260779677253632 |
|---|---|
| author | Silvi Ikawati Yung Fu Yen |
| author_facet | Silvi Ikawati Yung Fu Yen |
| author_sort | Silvi Ikawati |
| collection | DOAJ |
| description | The promoter plays an important role in the regulation of gene expression. The problem is some of binary vector that absence from promoter at cloning site. The cauliflower mosaic virus (CaMV) 35S promoter is a strong and constitutive promoter that widely used to produce transgenic organisms. In this experiment the cauliflower mosaic virus (CaMV) 35S promoter was spliced at upstream of DhPEX11-like for driving downstream transgenes DhPEX11-like expression used the technique of Overlap Extension by The Polymerase Chain Reaction. In gene splicing, internal primers are used to amplify some overlapping regions of both genes and then these internal primers are combined with the external primers in PCR process which allows amplification of the entire region. In the experiment, the recombinant PCR successfully spliced the 35S-DhPEX11 gene. This method is simple, rapid and reduced reagents used because it does not need many vector constructions. |
| format | Article |
| id | doaj-art-78aea13699a0458083cccd7f193f6979 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-78aea13699a0458083cccd7f193f69792025-08-20T01:55:33ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552015-06-01513843https://doi.org/10.21776/ub.jels.2015.005.01.07Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-LikeSilvi Ikawati0Yung Fu Yen1Universitas Brawijaya, Malang, IndonesiaNational Chiayi University, TaiwanThe promoter plays an important role in the regulation of gene expression. The problem is some of binary vector that absence from promoter at cloning site. The cauliflower mosaic virus (CaMV) 35S promoter is a strong and constitutive promoter that widely used to produce transgenic organisms. In this experiment the cauliflower mosaic virus (CaMV) 35S promoter was spliced at upstream of DhPEX11-like for driving downstream transgenes DhPEX11-like expression used the technique of Overlap Extension by The Polymerase Chain Reaction. In gene splicing, internal primers are used to amplify some overlapping regions of both genes and then these internal primers are combined with the external primers in PCR process which allows amplification of the entire region. In the experiment, the recombinant PCR successfully spliced the 35S-DhPEX11 gene. This method is simple, rapid and reduced reagents used because it does not need many vector constructions.https://jels.ub.ac.id/index.php/jels/article/view/152dhpex11-likegene splicingoligonucleotidepcrpromoter |
| spellingShingle | Silvi Ikawati Yung Fu Yen Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like Journal of Experimental Life Science dhpex11-like gene splicing oligonucleotide pcr promoter |
| title | Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like |
| title_full | Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like |
| title_fullStr | Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like |
| title_full_unstemmed | Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like |
| title_short | Technique of Overlap Extension by Polymerase Chain Reaction for Splicing Cauliflower Mosaic Virus (CaMV) 35S Promoter and DhPEX11-Like |
| title_sort | technique of overlap extension by polymerase chain reaction for splicing cauliflower mosaic virus camv 35s promoter and dhpex11 like |
| topic | dhpex11-like gene splicing oligonucleotide pcr promoter |
| url | https://jels.ub.ac.id/index.php/jels/article/view/152 |
| work_keys_str_mv | AT silviikawati techniqueofoverlapextensionbypolymerasechainreactionforsplicingcauliflowermosaicviruscamv35spromoteranddhpex11like AT yungfuyen techniqueofoverlapextensionbypolymerasechainreactionforsplicingcauliflowermosaicviruscamv35spromoteranddhpex11like |