Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China

IntroductionGranulated straw incorporation is a novel approach designed to enhance straw decomposition and improve soil fertility. However, the effects of different straw incorporation amounts under deep tillage and rotary tillage on soil available nutrients, soil organic carbon (SOC) fractions, bac...

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Main Authors: Jianxin Dong, Ping Wang, Ping Cong, Wenjing Song, Xuebo Zheng, Na Liu, Yi Wang, Xin Xiao, Zhen Zhai, Yuyi Li, Huancheng Pang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1520760/full
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author Jianxin Dong
Ping Wang
Ping Cong
Wenjing Song
Xuebo Zheng
Na Liu
Yi Wang
Xin Xiao
Zhen Zhai
Yuyi Li
Huancheng Pang
author_facet Jianxin Dong
Ping Wang
Ping Cong
Wenjing Song
Xuebo Zheng
Na Liu
Yi Wang
Xin Xiao
Zhen Zhai
Yuyi Li
Huancheng Pang
author_sort Jianxin Dong
collection DOAJ
description IntroductionGranulated straw incorporation is a novel approach designed to enhance straw decomposition and improve soil fertility. However, the effects of different straw incorporation amounts under deep tillage and rotary tillage on soil available nutrients, soil organic carbon (SOC) fractions, bacterial communities, and crop yield remain unclear.MethodsIn a 3-year field experiment, three granulated maize straw amounts (G1, 2,250; G2, 4,500; and G3, 6,750 kg hm−2) and two tillage methods (T, deep tillage; and R, rotary tillage) were applied to evaluate their impacts on SOC fractions, available nutrients, bacterial communities, and flue-cured tobacco yield.Results and DiscussionCompared with conventional tillage (RG0), granulated straw incorporation significantly increased SOC content. Over the 3 years, the SOC content in the 0–20 and 20–40 cm soil layers increased by 4.40%–23.46% and 5.36%–39.21% (p < 0.05), respectively. Moreover, the incorporation of higher straw amounts significantly increased the content of dissolved organic carbon (DOC) and microbial biomass carbon (MBC). Specifically, the RG3 treatment significantly increased DOC content in both soil layers in 2016 and 2017, while TG3 showed the greatest increase in 2018. In addition, RG2 and RG3 consistently enhanced MBC content across both layers throughout the 3 years. During the tobacco growing period, soil ammonium nitrogen (NH4+–N), nitrate nitrogen (NO3−–N), and available potassium (AK) contents increased with higher straw amounts in both soil layers. The RG2 treatment notably enhanced the bacterial α diversity and increased the relative abundance of phyla Firmicutes and Gemmatimonadota in the 20–40-cm layer. Network analysis identified AK as a key nutrient influencing bacterial community structure under both tillage methods. Structural equation modeling further revealed that SOC fractions were primarily regulated by nutrient factors under rotary tillage, while under deep tillage, bacterial richness and AK played dominant roles. To improve soil quality and crop productivity, the incorporation of a medium amount of granulated straw combined with rotary tillage is recommended as a sustainable practice for flue-cured tobacco cultivation.
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spelling doaj-art-78a56b3bb93645f88eb33ab890bd8f192025-08-20T03:09:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-07-011610.3389/fpls.2025.15207601520760Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East ChinaJianxin Dong0Ping Wang1Ping Cong2Wenjing Song3Xuebo Zheng4Na Liu5Yi Wang6Xin Xiao7Zhen Zhai8Yuyi Li9Huancheng Pang10Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaCollege of Tourism, Xinyang Normal University, Xinyang, ChinaZhucheng Experimental Station, Zhucheng Branch, Weifang Tobacco Co., Ltd., Weifang, ChinaKey Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Ecological Environment and Tobacco Quality, Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaIntroductionGranulated straw incorporation is a novel approach designed to enhance straw decomposition and improve soil fertility. However, the effects of different straw incorporation amounts under deep tillage and rotary tillage on soil available nutrients, soil organic carbon (SOC) fractions, bacterial communities, and crop yield remain unclear.MethodsIn a 3-year field experiment, three granulated maize straw amounts (G1, 2,250; G2, 4,500; and G3, 6,750 kg hm−2) and two tillage methods (T, deep tillage; and R, rotary tillage) were applied to evaluate their impacts on SOC fractions, available nutrients, bacterial communities, and flue-cured tobacco yield.Results and DiscussionCompared with conventional tillage (RG0), granulated straw incorporation significantly increased SOC content. Over the 3 years, the SOC content in the 0–20 and 20–40 cm soil layers increased by 4.40%–23.46% and 5.36%–39.21% (p < 0.05), respectively. Moreover, the incorporation of higher straw amounts significantly increased the content of dissolved organic carbon (DOC) and microbial biomass carbon (MBC). Specifically, the RG3 treatment significantly increased DOC content in both soil layers in 2016 and 2017, while TG3 showed the greatest increase in 2018. In addition, RG2 and RG3 consistently enhanced MBC content across both layers throughout the 3 years. During the tobacco growing period, soil ammonium nitrogen (NH4+–N), nitrate nitrogen (NO3−–N), and available potassium (AK) contents increased with higher straw amounts in both soil layers. The RG2 treatment notably enhanced the bacterial α diversity and increased the relative abundance of phyla Firmicutes and Gemmatimonadota in the 20–40-cm layer. Network analysis identified AK as a key nutrient influencing bacterial community structure under both tillage methods. Structural equation modeling further revealed that SOC fractions were primarily regulated by nutrient factors under rotary tillage, while under deep tillage, bacterial richness and AK played dominant roles. To improve soil quality and crop productivity, the incorporation of a medium amount of granulated straw combined with rotary tillage is recommended as a sustainable practice for flue-cured tobacco cultivation.https://www.frontiersin.org/articles/10.3389/fpls.2025.1520760/fullgranulated straw incorporationtillage methodsoil organic carbonsoil available nutrientsbacterial community
spellingShingle Jianxin Dong
Ping Wang
Ping Cong
Wenjing Song
Xuebo Zheng
Na Liu
Yi Wang
Xin Xiao
Zhen Zhai
Yuyi Li
Huancheng Pang
Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
Frontiers in Plant Science
granulated straw incorporation
tillage method
soil organic carbon
soil available nutrients
bacterial community
title Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
title_full Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
title_fullStr Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
title_full_unstemmed Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
title_short Granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in East China
title_sort granulated straw incorporation with rotary tillage increases the content of soil organic carbon fractions and available nutrients and shifts bacterial communities in east china
topic granulated straw incorporation
tillage method
soil organic carbon
soil available nutrients
bacterial community
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1520760/full
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