Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i>
Halotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress in crops. However, the current exploration and development of HT-PGPB remain insufficient to meet the increasing demands of agriculture. In this stud...
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2025-01-01
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| author | Longhao Sun Shanshan Sun Tianyang Liu Xinmin Lei Ruiqi Liu Junyi Zhang Shanshan Dai Jing Li Yanqin Ding |
| author_facet | Longhao Sun Shanshan Sun Tianyang Liu Xinmin Lei Ruiqi Liu Junyi Zhang Shanshan Dai Jing Li Yanqin Ding |
| author_sort | Longhao Sun |
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| description | Halotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress in crops. However, the current exploration and development of HT-PGPB remain insufficient to meet the increasing demands of agriculture. In this study, an HT-PGPB isolated from coastal saline-alkali soil in the Yellow River Delta was identified as <i>Glutamicibacter endophyticus</i> J2-5-19. The strain was capable of growing in media with up to 13% NaCl and producing proteases, siderophores, and the plant hormone IAA. Under 4‰ salt stress, inoculation with strain J2-5-19 significantly increased the wheat seed germination rate from 37.5% to 95%, enhanced the dry weight of maize seedlings by 41.92%, and notably improved the development of maize root systems. Moreover, this work presented the first whole-genome of <i>Glutamicibacter endophyticus</i>, revealing that <i>G. endophyticus</i> J2-5-19 resisted salt stress by expelling sodium ions and taking up potassium ions through Na<sup>+</sup>/H<sup>+</sup> antiporters and potassium uptake proteins, while also accumulating compatible solutes such as betaine, proline, and trehalose. Additionally, the genome contained multiple key plant growth-promoting genes, including those involved in IAA biosynthesis, siderophore production, and GABA synthesis. The findings provide a theoretical foundation and microbial resources for the development of specialized microbial inoculants for saline-alkali soils. |
| format | Article |
| id | doaj-art-78a411b552404304842732d68117b36b |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Microorganisms |
| spelling | doaj-art-78a411b552404304842732d68117b36b2025-01-24T13:43:04ZengMDPI AGMicroorganisms2076-26072025-01-0113120810.3390/microorganisms13010208Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i>Longhao Sun0Shanshan Sun1Tianyang Liu2Xinmin Lei3Ruiqi Liu4Junyi Zhang5Shanshan Dai6Jing Li7Yanqin Ding8Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaDepartment of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, ChinaHalotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress in crops. However, the current exploration and development of HT-PGPB remain insufficient to meet the increasing demands of agriculture. In this study, an HT-PGPB isolated from coastal saline-alkali soil in the Yellow River Delta was identified as <i>Glutamicibacter endophyticus</i> J2-5-19. The strain was capable of growing in media with up to 13% NaCl and producing proteases, siderophores, and the plant hormone IAA. Under 4‰ salt stress, inoculation with strain J2-5-19 significantly increased the wheat seed germination rate from 37.5% to 95%, enhanced the dry weight of maize seedlings by 41.92%, and notably improved the development of maize root systems. Moreover, this work presented the first whole-genome of <i>Glutamicibacter endophyticus</i>, revealing that <i>G. endophyticus</i> J2-5-19 resisted salt stress by expelling sodium ions and taking up potassium ions through Na<sup>+</sup>/H<sup>+</sup> antiporters and potassium uptake proteins, while also accumulating compatible solutes such as betaine, proline, and trehalose. Additionally, the genome contained multiple key plant growth-promoting genes, including those involved in IAA biosynthesis, siderophore production, and GABA synthesis. The findings provide a theoretical foundation and microbial resources for the development of specialized microbial inoculants for saline-alkali soils.https://www.mdpi.com/2076-2607/13/1/208saline-alkali soilHalotolerant plant growth-promoting bacteria<i>Glutamicibacter endophyticus</i>Genomic analysis |
| spellingShingle | Longhao Sun Shanshan Sun Tianyang Liu Xinmin Lei Ruiqi Liu Junyi Zhang Shanshan Dai Jing Li Yanqin Ding Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> Microorganisms saline-alkali soil Halotolerant plant growth-promoting bacteria <i>Glutamicibacter endophyticus</i> Genomic analysis |
| title | Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> |
| title_full | Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> |
| title_fullStr | Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> |
| title_full_unstemmed | Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> |
| title_short | Association Analysis of the Genomic and Functional Characteristics of Halotolerant <i>Glutamicibacter endophyticus</i> J2-5-19 from the Rhizosphere of <i>Suaeda salsa</i> |
| title_sort | association analysis of the genomic and functional characteristics of halotolerant i glutamicibacter endophyticus i j2 5 19 from the rhizosphere of i suaeda salsa i |
| topic | saline-alkali soil Halotolerant plant growth-promoting bacteria <i>Glutamicibacter endophyticus</i> Genomic analysis |
| url | https://www.mdpi.com/2076-2607/13/1/208 |
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