Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay
The enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used on a biochemistry auto analyzer (Cobas Integra 400) to determine serum creatinine at the laboratory of University Hospital – Pleven. The chara...
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Medical University - Pleven
2015-06-01
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| Series: | Journal of Biomedical & Clinical Research |
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| Online Access: | https://jbcr.arphahub.com/article/34369/download/pdf/ |
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| author | Irena Gencheva Adelaida Ruseva |
| author_facet | Irena Gencheva Adelaida Ruseva |
| author_sort | Irena Gencheva |
| collection | DOAJ |
| description | The enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used on a biochemistry auto analyzer (Cobas Integra 400) to determine serum creatinine at the laboratory of University Hospital – Pleven. The characteristics and reliability of this enzymatic method for creatinine were compared with the Jaffe kinetic method. Effects of some interfering substances like bilirubin and glucose on the Jaffe kinetic method and the enzymatic method were compared. Glucose and bilirubin inhibit the reaction between creatinine and alkaline picrate. Glucose slowly reduces picric acid to picrate, while the bilirubin present in a sample is oxidized to biliverdin under alkaline conditions. This leads to a decrease in absorbance at 520 nm. We measured creatinine in serum samples with the enzymatic method and the Jaffe kinetic method in samples divided into four groups: group I –samples without bilirubin and glucose ; group II –samples with high level of glucose; group III - samples with high level of bilirubin, group IV – all samples. For Group I, the correlation coefficient obtained by comparing the enzymatic creatinine method and Jaffe's kinetic method was R = 0.983. There was a very good agreement between the two methods in terms of correlation coefficient even in the samples with high levels of glucose or bilirubin. |
| format | Article |
| id | doaj-art-78a2b494bfe94cd6843d7b226ef75b77 |
| institution | Kabale University |
| issn | 1313-9053 |
| language | English |
| publishDate | 2015-06-01 |
| publisher | Medical University - Pleven |
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| series | Journal of Biomedical & Clinical Research |
| spelling | doaj-art-78a2b494bfe94cd6843d7b226ef75b772025-08-20T03:56:42ZengMedical University - PlevenJournal of Biomedical & Clinical Research1313-90532015-06-0181353910.1515/jbcr-2015-014934369Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine AssayIrena GenchevaAdelaida RusevaThe enzymatic method for the determination of serum creatinine is accepted as one of the most accurate methods in a clinical laboratory. This method was used on a biochemistry auto analyzer (Cobas Integra 400) to determine serum creatinine at the laboratory of University Hospital – Pleven. The characteristics and reliability of this enzymatic method for creatinine were compared with the Jaffe kinetic method. Effects of some interfering substances like bilirubin and glucose on the Jaffe kinetic method and the enzymatic method were compared. Glucose and bilirubin inhibit the reaction between creatinine and alkaline picrate. Glucose slowly reduces picric acid to picrate, while the bilirubin present in a sample is oxidized to biliverdin under alkaline conditions. This leads to a decrease in absorbance at 520 nm. We measured creatinine in serum samples with the enzymatic method and the Jaffe kinetic method in samples divided into four groups: group I –samples without bilirubin and glucose ; group II –samples with high level of glucose; group III - samples with high level of bilirubin, group IV – all samples. For Group I, the correlation coefficient obtained by comparing the enzymatic creatinine method and Jaffe's kinetic method was R = 0.983. There was a very good agreement between the two methods in terms of correlation coefficient even in the samples with high levels of glucose or bilirubin.https://jbcr.arphahub.com/article/34369/download/pdf/creatinineenzymatic assayJaffe kinetic method |
| spellingShingle | Irena Gencheva Adelaida Ruseva Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay Journal of Biomedical & Clinical Research creatinine enzymatic assay Jaffe kinetic method |
| title | Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay |
| title_full | Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay |
| title_fullStr | Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay |
| title_full_unstemmed | Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay |
| title_short | Effects Of Glucose And Bilirubin On The Kinetic Jaffe's And The Enzymatic Methods For Serum Creatinine Assay |
| title_sort | effects of glucose and bilirubin on the kinetic jaffe 039 s and the enzymatic methods for serum creatinine assay |
| topic | creatinine enzymatic assay Jaffe kinetic method |
| url | https://jbcr.arphahub.com/article/34369/download/pdf/ |
| work_keys_str_mv | AT irenagencheva effectsofglucoseandbilirubinonthekineticjaffe039sandtheenzymaticmethodsforserumcreatinineassay AT adelaidaruseva effectsofglucoseandbilirubinonthekineticjaffe039sandtheenzymaticmethodsforserumcreatinineassay |