Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates
Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neon...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/4884537 |
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| author | Ahmed Omran Hazem Sobh Mohamed Osama Abdalla Sonya El-Sharkawy Ahmed R. Rezk Abdelmoneim Khashana |
| author_facet | Ahmed Omran Hazem Sobh Mohamed Osama Abdalla Sonya El-Sharkawy Ahmed R. Rezk Abdelmoneim Khashana |
| author_sort | Ahmed Omran |
| collection | DOAJ |
| description | Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control (220±150 vs. 18±9 pg/ml, P<0.001), (316±198 vs. 23.7±14 pg/ml, P<0.001), (78.2±34 vs. 3.3±1.7 mg/L, P<0.001), (11.2±0.9 vs. 8.6±0.4 fL), and (7.08±3.3 vs. 0.4±0.2, P<0.001), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff value≥9.2 fL. CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff value>0.9. Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates. |
| format | Article |
| id | doaj-art-7898641a115f4065b283c3a3cfafc6c8 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-7898641a115f4065b283c3a3cfafc6c82025-08-20T02:07:30ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/48845374884537Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term NeonatesAhmed Omran0Hazem Sobh1Mohamed Osama Abdalla2Sonya El-Sharkawy3Ahmed R. Rezk4Abdelmoneim Khashana5Department of Pediatrics & Neonatology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartment of Pediatrics & Neonatology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptClinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartments of Pediatrics & Neonatology, Faculty of Medicine, Port Said University, Port Said, EgyptDepartment of Pediatrics, Ain Shams University, Cairo, EgyptDepartment of Pediatrics & Neonatology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptSalivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control (220±150 vs. 18±9 pg/ml, P<0.001), (316±198 vs. 23.7±14 pg/ml, P<0.001), (78.2±34 vs. 3.3±1.7 mg/L, P<0.001), (11.2±0.9 vs. 8.6±0.4 fL), and (7.08±3.3 vs. 0.4±0.2, P<0.001), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff value≥9.2 fL. CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff value>0.9. Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates.http://dx.doi.org/10.1155/2021/4884537 |
| spellingShingle | Ahmed Omran Hazem Sobh Mohamed Osama Abdalla Sonya El-Sharkawy Ahmed R. Rezk Abdelmoneim Khashana Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates Journal of Immunology Research |
| title | Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates |
| title_full | Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates |
| title_fullStr | Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates |
| title_full_unstemmed | Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates |
| title_short | Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates |
| title_sort | salivary and serum interleukin 10 c reactive protein mean platelet volume and crp mpv ratio in the diagnosis of late onset neonatal sepsis in full term neonates |
| url | http://dx.doi.org/10.1155/2021/4884537 |
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