The DoGA consortium expression atlas of promoters and genes in 100 canine tissues

Abstract The dog, Canis lupus familiaris, is an important model for studying human diseases. Unlike many model organisms, the dog genome has a comparatively poor functional annotation, which hampers gene discovery for development, morphology, disease, and behavior. To fill this gap, we established a...

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Main Authors: Matthias Hörtenhuber, Marjo K. Hytönen, Abdul Kadir Mukarram, Meharji Arumilli, César L. Araujo, Ileana Quintero, Pernilla Syrjä, Niina Airas, Maria Kaukonen, Kaisa Kyöstilä, Julia Niskanen, Tarja S. Jokinen, Faezeh Mottaghitalab, Işıl Takan, Noora Salokorpi, Amitha Raman, Irene Stevens, Antti Iivanainen, Masahito Yoshihara, Oleg Gusev, Danika Bannasch, Antti Sukura, Jeffrey J. Schoenebeck, DoGA Consortium, Sini Ezer, Shintaro Katayama, Carsten O. Daub, Juha Kere, Hannes Lohi
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-52798-1
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author Matthias Hörtenhuber
Marjo K. Hytönen
Abdul Kadir Mukarram
Meharji Arumilli
César L. Araujo
Ileana Quintero
Pernilla Syrjä
Niina Airas
Maria Kaukonen
Kaisa Kyöstilä
Julia Niskanen
Tarja S. Jokinen
Faezeh Mottaghitalab
Işıl Takan
Noora Salokorpi
Amitha Raman
Irene Stevens
Antti Iivanainen
Masahito Yoshihara
Oleg Gusev
Danika Bannasch
Antti Sukura
Jeffrey J. Schoenebeck
DoGA Consortium
Sini Ezer
Shintaro Katayama
Carsten O. Daub
Juha Kere
Hannes Lohi
author_facet Matthias Hörtenhuber
Marjo K. Hytönen
Abdul Kadir Mukarram
Meharji Arumilli
César L. Araujo
Ileana Quintero
Pernilla Syrjä
Niina Airas
Maria Kaukonen
Kaisa Kyöstilä
Julia Niskanen
Tarja S. Jokinen
Faezeh Mottaghitalab
Işıl Takan
Noora Salokorpi
Amitha Raman
Irene Stevens
Antti Iivanainen
Masahito Yoshihara
Oleg Gusev
Danika Bannasch
Antti Sukura
Jeffrey J. Schoenebeck
DoGA Consortium
Sini Ezer
Shintaro Katayama
Carsten O. Daub
Juha Kere
Hannes Lohi
author_sort Matthias Hörtenhuber
collection DOAJ
description Abstract The dog, Canis lupus familiaris, is an important model for studying human diseases. Unlike many model organisms, the dog genome has a comparatively poor functional annotation, which hampers gene discovery for development, morphology, disease, and behavior. To fill this gap, we established a comprehensive tissue biobank for both the dog and wolf samples. The biobank consists of 5485 samples representing 132 tissues from 13 dogs, 12 dog embryos, and 24 wolves. In a subset of 100 tissues from nine dogs and 12 embryos, we characterized gene expression activity for each promoter, including alternative and novel, i.e., previously not annotated, promoter regions, using the 5’ targeting RNA sequencing technology STRT2-seq. We identified over 100,000 promoter region candidates in the recent canine genome assembly, CanFam4, including over 45,000 highly reproducible sites with gene expression and respective tissue enrichment levels. We provide a promoter and gene expression atlas with interactive, open data resources, including a data coordination center and genome browser track hubs. We demonstrated the applicability of Dog Genome Annotation (DoGA) data and resources using multiple examples spanning canine embryonic development, morphology and behavior, and diseases across species.
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spelling doaj-art-7894452dee5b4ca9b19fbe1f626becc82025-08-20T02:11:25ZengNature PortfolioNature Communications2041-17232024-10-0115111210.1038/s41467-024-52798-1The DoGA consortium expression atlas of promoters and genes in 100 canine tissuesMatthias Hörtenhuber0Marjo K. Hytönen1Abdul Kadir Mukarram2Meharji Arumilli3César L. Araujo4Ileana Quintero5Pernilla Syrjä6Niina Airas7Maria Kaukonen8Kaisa Kyöstilä9Julia Niskanen10Tarja S. Jokinen11Faezeh Mottaghitalab12Işıl Takan13Noora Salokorpi14Amitha Raman15Irene Stevens16Antti Iivanainen17Masahito Yoshihara18Oleg Gusev19Danika Bannasch20Antti Sukura21Jeffrey J. Schoenebeck22DoGA ConsortiumSini Ezer23Shintaro Katayama24Carsten O. Daub25Juha Kere26Hannes Lohi27Department of Medicine, Huddinge, Karolinska InstitutetDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Equine and Small Animal Medicine, University of HelsinkiDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Veterinary Biosciences, University of HelsinkiDepartment of Medicine, Huddinge, Karolinska InstitutetIntractable Disease Research Center, Graduate School of Medicine, Juntendo UniversityDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of CaliforniaDepartment of Veterinary Biosciences, University of HelsinkiRoslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghFolkhälsan Research CenterDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Medicine, Huddinge, Karolinska InstitutetDepartment of Veterinary Biosciences, University of HelsinkiAbstract The dog, Canis lupus familiaris, is an important model for studying human diseases. Unlike many model organisms, the dog genome has a comparatively poor functional annotation, which hampers gene discovery for development, morphology, disease, and behavior. To fill this gap, we established a comprehensive tissue biobank for both the dog and wolf samples. The biobank consists of 5485 samples representing 132 tissues from 13 dogs, 12 dog embryos, and 24 wolves. In a subset of 100 tissues from nine dogs and 12 embryos, we characterized gene expression activity for each promoter, including alternative and novel, i.e., previously not annotated, promoter regions, using the 5’ targeting RNA sequencing technology STRT2-seq. We identified over 100,000 promoter region candidates in the recent canine genome assembly, CanFam4, including over 45,000 highly reproducible sites with gene expression and respective tissue enrichment levels. We provide a promoter and gene expression atlas with interactive, open data resources, including a data coordination center and genome browser track hubs. We demonstrated the applicability of Dog Genome Annotation (DoGA) data and resources using multiple examples spanning canine embryonic development, morphology and behavior, and diseases across species.https://doi.org/10.1038/s41467-024-52798-1
spellingShingle Matthias Hörtenhuber
Marjo K. Hytönen
Abdul Kadir Mukarram
Meharji Arumilli
César L. Araujo
Ileana Quintero
Pernilla Syrjä
Niina Airas
Maria Kaukonen
Kaisa Kyöstilä
Julia Niskanen
Tarja S. Jokinen
Faezeh Mottaghitalab
Işıl Takan
Noora Salokorpi
Amitha Raman
Irene Stevens
Antti Iivanainen
Masahito Yoshihara
Oleg Gusev
Danika Bannasch
Antti Sukura
Jeffrey J. Schoenebeck
DoGA Consortium
Sini Ezer
Shintaro Katayama
Carsten O. Daub
Juha Kere
Hannes Lohi
The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
Nature Communications
title The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
title_full The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
title_fullStr The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
title_full_unstemmed The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
title_short The DoGA consortium expression atlas of promoters and genes in 100 canine tissues
title_sort doga consortium expression atlas of promoters and genes in 100 canine tissues
url https://doi.org/10.1038/s41467-024-52798-1
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