Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins
Metallodrugs are widely used in the treatment of several diseases, including cancer. Many bloodstream proteins exhibit high affinities for metals and metal-based compounds, and these interactions have been shown to impact metallodrug pharmacokinetics and pharmacodynamics, ultimately influencing thei...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/bca/5551967 |
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| author | Quim Peña Iker Zapirain-Gysling A. Jalila Simaan Mercè Capdevila Olga Iranzo Òscar Palacios Jean-Didier Maréchal Pau Bayón |
| author_facet | Quim Peña Iker Zapirain-Gysling A. Jalila Simaan Mercè Capdevila Olga Iranzo Òscar Palacios Jean-Didier Maréchal Pau Bayón |
| author_sort | Quim Peña |
| collection | DOAJ |
| description | Metallodrugs are widely used in the treatment of several diseases, including cancer. Many bloodstream proteins exhibit high affinities for metals and metal-based compounds, and these interactions have been shown to impact metallodrug pharmacokinetics and pharmacodynamics, ultimately influencing their therapeutic performance. Several spectroscopic, spectrometric, and computational techniques have been used to further understand drug–protein binding modes at the molecular level over the last few years. However, there is still plenty of room to ascertain the full potential of integrated experimental–computational methodologies in the field of metallodrugs. These studies have mostly been limited to a few metal-containing systems, such as platinates and vanadium drugs. In this work, we aim to expand and validate the use of combined experimental–theoretical approaches in studying copper (Cu)-based drug interactions with proteins. To do so, a tetracoordinated Cu(II) complex has been employed as a practical case study, and its interactions with several relevant proteins using different experimental and computational techniques have been evaluated, including electron paramagnetic resonance spectroscopy, mass spectrometry, density functional theory, and protein–ligand docking calculations. Experimental data highlight interactions of the Cu(II) complex with albumin, myoglobin, and cytochrome C. Further insights into the preferential modes of binding were evaluated by density functional theory and docking calculations, which revealed three main outer surface binding sites for both albumin and myoglobin, as well as three preferential inner pockets in the case of albumin. The most relevant binding modes found for both proteins include noncoordinative interactions with the Cu(II) dimeric structure intact upon binding, as well as metal–ligand exchange and direct metal–amino acid coordination, mainly to glutamate/aspartate residues after cleavage of the dimer. Altogether, an example of the applicability of a mixed experimental–theoretical framework in the study of Cu-based drug interactions with relevant proteins is shown. |
| format | Article |
| id | doaj-art-7887fecbdb334483bd4fff63f2976b29 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-7887fecbdb334483bd4fff63f2976b292025-08-20T03:24:57ZengWileyBioinorganic Chemistry and Applications1687-479X2025-01-01202510.1155/bca/5551967Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With ProteinsQuim Peña0Iker Zapirain-Gysling1A. Jalila Simaan2Mercè Capdevila3Olga Iranzo4Òscar Palacios5Jean-Didier Maréchal6Pau Bayón7Chemistry DepartmentChemistry DepartmentAix Marseille UniversityChemistry DepartmentAix Marseille UniversityChemistry DepartmentChemistry DepartmentChemistry DepartmentMetallodrugs are widely used in the treatment of several diseases, including cancer. Many bloodstream proteins exhibit high affinities for metals and metal-based compounds, and these interactions have been shown to impact metallodrug pharmacokinetics and pharmacodynamics, ultimately influencing their therapeutic performance. Several spectroscopic, spectrometric, and computational techniques have been used to further understand drug–protein binding modes at the molecular level over the last few years. However, there is still plenty of room to ascertain the full potential of integrated experimental–computational methodologies in the field of metallodrugs. These studies have mostly been limited to a few metal-containing systems, such as platinates and vanadium drugs. In this work, we aim to expand and validate the use of combined experimental–theoretical approaches in studying copper (Cu)-based drug interactions with proteins. To do so, a tetracoordinated Cu(II) complex has been employed as a practical case study, and its interactions with several relevant proteins using different experimental and computational techniques have been evaluated, including electron paramagnetic resonance spectroscopy, mass spectrometry, density functional theory, and protein–ligand docking calculations. Experimental data highlight interactions of the Cu(II) complex with albumin, myoglobin, and cytochrome C. Further insights into the preferential modes of binding were evaluated by density functional theory and docking calculations, which revealed three main outer surface binding sites for both albumin and myoglobin, as well as three preferential inner pockets in the case of albumin. The most relevant binding modes found for both proteins include noncoordinative interactions with the Cu(II) dimeric structure intact upon binding, as well as metal–ligand exchange and direct metal–amino acid coordination, mainly to glutamate/aspartate residues after cleavage of the dimer. Altogether, an example of the applicability of a mixed experimental–theoretical framework in the study of Cu-based drug interactions with relevant proteins is shown.http://dx.doi.org/10.1155/bca/5551967 |
| spellingShingle | Quim Peña Iker Zapirain-Gysling A. Jalila Simaan Mercè Capdevila Olga Iranzo Òscar Palacios Jean-Didier Maréchal Pau Bayón Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins Bioinorganic Chemistry and Applications |
| title | Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins |
| title_full | Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins |
| title_fullStr | Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins |
| title_full_unstemmed | Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins |
| title_short | Combining Experimental and Computational Tools to Unravel Copper-Based Metallodrug Interactions With Proteins |
| title_sort | combining experimental and computational tools to unravel copper based metallodrug interactions with proteins |
| url | http://dx.doi.org/10.1155/bca/5551967 |
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