Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice

Lodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice (Oryza sativa L.) production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related...

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Main Authors: Wu-jun ZHANG, Long-mei WU, Yan-feng DING, Fei WENG, Xiao-ran WU, Gang-hua LI, Zheng-hui LIU, She TANG, Cheng-qiang DING, Shao-hua WANG
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2016-05-01
Series:Journal of Integrative Agriculture
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095311915611662
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author Wu-jun ZHANG
Long-mei WU
Yan-feng DING
Fei WENG
Xiao-ran WU
Gang-hua LI
Zheng-hui LIU
She TANG
Cheng-qiang DING
Shao-hua WANG
author_facet Wu-jun ZHANG
Long-mei WU
Yan-feng DING
Fei WENG
Xiao-ran WU
Gang-hua LI
Zheng-hui LIU
She TANG
Cheng-qiang DING
Shao-hua WANG
author_sort Wu-jun ZHANG
collection DOAJ
description Lodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice (Oryza sativa L.) production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related arrangement in culm tissue and response to top-dressing nitrogen (N) fertilizer. Field experiments were conducted in Danyang County, Jiangsu Province, China, by using two japonica rice varieties Wuyunjing 23 (lodging-resistance variety) and W3668 (lodging-susceptible variety) with three top-dressing N fertilizer rates (0, 135 and 270 kg N ha−1) in 2013 and 2014. Lodging related physical parameters, morphological characteristics and stem carbohydrate components were investigated at 30 d after full heading stage. Results showed that with increasing N fertilizer rates, the lodging rate and lodging index increased rapidly primarily due to significant reduction of breaking strength in two japonica rice varieties. Correlation analysis revealed that breaking strength was significantly and positively correlated with bending stress, but negatively correlated with section modulus, except for significant correlation at W3668 in 2014. Higher stem plumpness status and structural carbohydrate contents significantly enhanced stem stiffness, despite of lower non-structural carbohydrate. With higher N fertilizer rate, the culm wall thickness was almost identical, and culm diameter increased slightly. The structural carbohydrates, especially for lignin content in culm, reduced significantly under high N rate. Further histochemical staining analysis revealed that high N treatments decreased the lignin deposition rapidly in the sclerenchyma cells of mechanical tissue, large vascular bundle and small vascular bundle region, which were consistent with reduction of bending stress, especially for W3668 and thus, resulted in poor stem strength and higher lodging index. These results suggested that structural carbohydrate plays a vital role for improving stem strength in japonica rice. N rate decreased lodging resistance primarily due to poor stem stiffness, by reducing structural carbohydrate content and lignin deposition in the secondary cell wall of lower internode culm tissue.
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spelling doaj-art-787fdc9d048e4b39862a7121f036eac62025-08-20T03:57:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-05-01155992100410.1016/S2095-3119(15)61166-2Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica riceWu-jun ZHANG0Long-mei WU1Yan-feng DING2Fei WENG3Xiao-ran WU4Gang-hua LI5Zheng-hui LIU6She TANG7Cheng-qiang DING8Shao-hua WANG9Jiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaCorrespondence LI Gang-hua, Tel/Fax: +86-25-84396475; Jiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Collaborative Innovation Center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology Center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaLodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice (Oryza sativa L.) production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related arrangement in culm tissue and response to top-dressing nitrogen (N) fertilizer. Field experiments were conducted in Danyang County, Jiangsu Province, China, by using two japonica rice varieties Wuyunjing 23 (lodging-resistance variety) and W3668 (lodging-susceptible variety) with three top-dressing N fertilizer rates (0, 135 and 270 kg N ha−1) in 2013 and 2014. Lodging related physical parameters, morphological characteristics and stem carbohydrate components were investigated at 30 d after full heading stage. Results showed that with increasing N fertilizer rates, the lodging rate and lodging index increased rapidly primarily due to significant reduction of breaking strength in two japonica rice varieties. Correlation analysis revealed that breaking strength was significantly and positively correlated with bending stress, but negatively correlated with section modulus, except for significant correlation at W3668 in 2014. Higher stem plumpness status and structural carbohydrate contents significantly enhanced stem stiffness, despite of lower non-structural carbohydrate. With higher N fertilizer rate, the culm wall thickness was almost identical, and culm diameter increased slightly. The structural carbohydrates, especially for lignin content in culm, reduced significantly under high N rate. Further histochemical staining analysis revealed that high N treatments decreased the lignin deposition rapidly in the sclerenchyma cells of mechanical tissue, large vascular bundle and small vascular bundle region, which were consistent with reduction of bending stress, especially for W3668 and thus, resulted in poor stem strength and higher lodging index. These results suggested that structural carbohydrate plays a vital role for improving stem strength in japonica rice. N rate decreased lodging resistance primarily due to poor stem stiffness, by reducing structural carbohydrate content and lignin deposition in the secondary cell wall of lower internode culm tissue.http://www.sciencedirect.com/science/article/pii/S2095311915611662japonica ricelodging resistancenitrogenstem strengthstructural carbohydrate
spellingShingle Wu-jun ZHANG
Long-mei WU
Yan-feng DING
Fei WENG
Xiao-ran WU
Gang-hua LI
Zheng-hui LIU
She TANG
Cheng-qiang DING
Shao-hua WANG
Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
Journal of Integrative Agriculture
japonica rice
lodging resistance
nitrogen
stem strength
structural carbohydrate
title Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
title_full Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
title_fullStr Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
title_full_unstemmed Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
title_short Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
title_sort top dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice
topic japonica rice
lodging resistance
nitrogen
stem strength
structural carbohydrate
url http://www.sciencedirect.com/science/article/pii/S2095311915611662
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