Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province
In order to investigate the infection status of <i>Cryptosporidium</i> in <i>O. curzoniae</i> and <i>B. grunniens</i> in Zoige County, Sichuan Province, fecal samples from <i>B. grunniens</i> and gastrointestinal contents from captured <i>O. curz...
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MDPI AG
2025-07-01
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| author | Tian-Cai Tang Ri-Hong Jike Liang-Quan Zhu Chao-Xi Chen Li-Li Hao |
| author_facet | Tian-Cai Tang Ri-Hong Jike Liang-Quan Zhu Chao-Xi Chen Li-Li Hao |
| author_sort | Tian-Cai Tang |
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| description | In order to investigate the infection status of <i>Cryptosporidium</i> in <i>O. curzoniae</i> and <i>B. grunniens</i> in Zoige County, Sichuan Province, fecal samples from <i>B. grunniens</i> and gastrointestinal contents from captured <i>O. curzoniae</i> were collected between March and December 2023 from five townships (Dazhasi, Axi, Hongxing, Tangke, and Maixi). Genomic DNA was extracted, and nested PCR targeting the small subunit (SSU) rRNA gene of <i>Cryptosporidium</i> was performed. PCR-positive products were sequenced, trimmed, aligned, and subjected to phylogenetic analysis to determine species and genotypes. A total of 242 samples were obtained, of which 20 were <i>Cryptosporidium SSU rRNA</i>-positive, yielding an overall detection rate of 8.3% (20/242). The detection rates of <i>O. curzoniae</i> and <i>B. grunniens</i> were 7.0% (8/114) and 9.4% (12/128), respectively. Among the five sampling sites, Maixi town exhibited the highest detection rate (32.4%, 11/44), followed by Hongxing town (15.2%, 7/46) and Tangke town (4.6%, 2/44). Phylogenetic analysis detected an unidentified <i>Cryptosporidium</i> sp. in <i>O. curzoniae</i>, while <i>C. bovis</i> (<i>n</i> = 10) and <i>C. ryanae</i> (<i>n</i> = 2) were detected in <i>B. grunniens</i>. These findings demonstrate that <i>Cryptosporidium</i> infections are present in both <i>O. curzoniae</i> and <i>B. grunniens</i> in Zoige County, with notable differences in infection rates and species composition. Continued surveillance of <i>Cryptosporidium</i> in local livestock and wildlife is warranted to provide critical data for regional public health management. |
| format | Article |
| id | doaj-art-786b2c35bcee43fb83bce8c821bbcab3 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-786b2c35bcee43fb83bce8c821bbcab32025-08-20T03:55:48ZengMDPI AGAnimals2076-26152025-07-011514214010.3390/ani15142140Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan ProvinceTian-Cai Tang0Ri-Hong Jike1Liang-Quan Zhu2Chao-Xi Chen3Li-Li Hao4College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaAgricultural and Rural Bureau of Liangshan Yi Autonomous Prefecture of Sichuan Province, Liangshan 615000, ChinaChina Institute of Veterinary Drug Control (IVDC), Beijing 100081, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaIn order to investigate the infection status of <i>Cryptosporidium</i> in <i>O. curzoniae</i> and <i>B. grunniens</i> in Zoige County, Sichuan Province, fecal samples from <i>B. grunniens</i> and gastrointestinal contents from captured <i>O. curzoniae</i> were collected between March and December 2023 from five townships (Dazhasi, Axi, Hongxing, Tangke, and Maixi). Genomic DNA was extracted, and nested PCR targeting the small subunit (SSU) rRNA gene of <i>Cryptosporidium</i> was performed. PCR-positive products were sequenced, trimmed, aligned, and subjected to phylogenetic analysis to determine species and genotypes. A total of 242 samples were obtained, of which 20 were <i>Cryptosporidium SSU rRNA</i>-positive, yielding an overall detection rate of 8.3% (20/242). The detection rates of <i>O. curzoniae</i> and <i>B. grunniens</i> were 7.0% (8/114) and 9.4% (12/128), respectively. Among the five sampling sites, Maixi town exhibited the highest detection rate (32.4%, 11/44), followed by Hongxing town (15.2%, 7/46) and Tangke town (4.6%, 2/44). Phylogenetic analysis detected an unidentified <i>Cryptosporidium</i> sp. in <i>O. curzoniae</i>, while <i>C. bovis</i> (<i>n</i> = 10) and <i>C. ryanae</i> (<i>n</i> = 2) were detected in <i>B. grunniens</i>. These findings demonstrate that <i>Cryptosporidium</i> infections are present in both <i>O. curzoniae</i> and <i>B. grunniens</i> in Zoige County, with notable differences in infection rates and species composition. Continued surveillance of <i>Cryptosporidium</i> in local livestock and wildlife is warranted to provide critical data for regional public health management.https://www.mdpi.com/2076-2615/15/14/2140<i>Ochotona curzoniae</i><i>Bos grunniens</i><i>Cryptosporidium</i><i>SSU rRNA</i>epidemiological survey |
| spellingShingle | Tian-Cai Tang Ri-Hong Jike Liang-Quan Zhu Chao-Xi Chen Li-Li Hao Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province Animals <i>Ochotona curzoniae</i> <i>Bos grunniens</i> <i>Cryptosporidium</i> <i>SSU rRNA</i> epidemiological survey |
| title | Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province |
| title_full | Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province |
| title_fullStr | Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province |
| title_full_unstemmed | Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province |
| title_short | Molecular Epidemiological Survey of <i>Cryptosporidium</i> in <i>Ochotona curzoniae</i> and <i>Bos grunniens</i> of Zoige County, Sichuan Province |
| title_sort | molecular epidemiological survey of i cryptosporidium i in i ochotona curzoniae i and i bos grunniens i of zoige county sichuan province |
| topic | <i>Ochotona curzoniae</i> <i>Bos grunniens</i> <i>Cryptosporidium</i> <i>SSU rRNA</i> epidemiological survey |
| url | https://www.mdpi.com/2076-2615/15/14/2140 |
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