Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco
Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from...
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The Journal of Infection in Developing Countries
2019-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10675 |
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| author | Chaimae Elmouaden Amin Laglaoui Latifa Ennanei Mohammed Bakkali Mohammed Abid |
| author_facet | Chaimae Elmouaden Amin Laglaoui Latifa Ennanei Mohammed Bakkali Mohammed Abid |
| author_sort | Chaimae Elmouaden |
| collection | DOAJ |
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Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from nosocomial and community-acquired infections in the Northwestern of Morocco.
Methodology: A total of 155 of Pseudomonas aeruginosa strains were collected (January 2015 - December 2016) from nosocomial and community-acquired infections at hospital centers and clinical laboratories in the Northwestern of Morocco. Antimicrobial susceptibility test was performed by the standard disk diffusion method. In addition, PCR assays were used for screening five virulence encoding genes (lasB, algD, plcH, exoA, and exoS).
Results: Our results revealed that high level of antimicrobial resistance was detected towards aztreonam (27.1%) followed by meropenem (14.2%). The resistance to imipenem was significantly higher in strains isolated from nosocomial infections (12.7%) than strains isolated from community-acquired infections (1.5%). The results highlighted that lasB (98.7%) and exoS (98.7%) were the most frequent virulence genes.
Conclusions: This survey provides data about phenotypic and genotypic properties of Pseudomonas aeruginosa emerged in the Northwestern of Morocco. It could be helpful for the health workers to improve infection control measures and to establish a surveillance system.
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| format | Article |
| id | doaj-art-786582b9237642a7adea90d2a7e88116 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-786582b9237642a7adea90d2a7e881162025-08-20T02:27:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-10-01131010.3855/jidc.10675Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of MoroccoChaimae Elmouaden0Amin Laglaoui1Latifa Ennanei2Mohammed Bakkali3Mohammed Abid4Department of Research, Institut Pasteur du Maroc, Tangier, MoroccoBiotechnology and Biomolecule Engineering Team, Department of Biology, Faculty of Science and Technology, Abdelmalek Essaadi University, Tangier, MoroccoDepartment of Research, Institut Pasteur du Maroc, Tangier, MoroccoBiotechnology and Biomolecule Engineering Team, Department of Biology, Faculty of Science and Technology, Abdelmalek Essaadi University, Tangier, MoroccoDepartment of Research, Institut Pasteur du Maroc, Tangier, Morocco Introduction: Pseudomonas aeruginosa is an ubiquitous bacterium causes various community-acquired and nosocomial infections. In this investigation, we aimed to screen the antibiotic susceptibility patterns and the prevalence of virulence factor genes in a set of Pseudomonas aeruginosa isolated from nosocomial and community-acquired infections in the Northwestern of Morocco. Methodology: A total of 155 of Pseudomonas aeruginosa strains were collected (January 2015 - December 2016) from nosocomial and community-acquired infections at hospital centers and clinical laboratories in the Northwestern of Morocco. Antimicrobial susceptibility test was performed by the standard disk diffusion method. In addition, PCR assays were used for screening five virulence encoding genes (lasB, algD, plcH, exoA, and exoS). Results: Our results revealed that high level of antimicrobial resistance was detected towards aztreonam (27.1%) followed by meropenem (14.2%). The resistance to imipenem was significantly higher in strains isolated from nosocomial infections (12.7%) than strains isolated from community-acquired infections (1.5%). The results highlighted that lasB (98.7%) and exoS (98.7%) were the most frequent virulence genes. Conclusions: This survey provides data about phenotypic and genotypic properties of Pseudomonas aeruginosa emerged in the Northwestern of Morocco. It could be helpful for the health workers to improve infection control measures and to establish a surveillance system. https://jidc.org/index.php/journal/article/view/10675Pseudomonas aeruginosanosocomial infectionscommunity-acquired infectionsantimicrobial resistancevirulence genesMorocco |
| spellingShingle | Chaimae Elmouaden Amin Laglaoui Latifa Ennanei Mohammed Bakkali Mohammed Abid Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco Journal of Infection in Developing Countries Pseudomonas aeruginosa nosocomial infections community-acquired infections antimicrobial resistance virulence genes Morocco |
| title | Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco |
| title_full | Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco |
| title_fullStr | Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco |
| title_full_unstemmed | Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco |
| title_short | Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco |
| title_sort | virulence genes and antibiotic resistance of pseudomonas aeruginosa isolated from patients in the northwestern of morocco |
| topic | Pseudomonas aeruginosa nosocomial infections community-acquired infections antimicrobial resistance virulence genes Morocco |
| url | https://jidc.org/index.php/journal/article/view/10675 |
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