Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes
Preclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate whether donor...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1461648 |
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| _version_ | 1832554294158557184 |
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| author | Flavia Bruna David Contador Paulette Conget Benjamín Erranz Claudia L. Sossa Martha L. Arango-Rodríguez |
| author_facet | Flavia Bruna David Contador Paulette Conget Benjamín Erranz Claudia L. Sossa Martha L. Arango-Rodríguez |
| author_sort | Flavia Bruna |
| collection | DOAJ |
| description | Preclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate whether donor age influences this potential. Two different doses of bone marrow MSCs (BM-MSCs) from young, adult, or old mouse donors or two doses of their acellular derivatives mesenchymal stromal cells (acd-MSCs) were intradermally injected around wounds in the midline of C57BL/6 mice. Every two days, wound healing was macroscopically assessed (wound closure) and microscopically assessed (reepithelialization, dermal-epidermal junction, skin appendage regeneration, granulation tissue, leukocyte infiltration, and density dermal collagen fibers) after 12 days from MSC transplant. Significant differences in the wound closure kinetic, quality, and healing of skin regenerated were observed in lesions which received BM-MSCs from different ages or their acd-MSCs compared to lesions which received vehicle. Nevertheless, our data shows that adult’s BM-MSCs or their acd-MSCs were the most efficient for recovery of most parameters analyzed. Our data suggest that MSC efficacy was negatively affected by donor age, where the treatment with adult’s BM-MSCs or their acd-MSCs in cutaneous wound promotes a better tissue repair/regeneration. This is due to their paracrine factors secretion. |
| format | Article |
| id | doaj-art-7864ac068c604723a7c90766a4a540ed |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7864ac068c604723a7c90766a4a540ed2025-02-03T05:51:53ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/14616481461648Regenerative Potential of Mesenchymal Stromal Cells: Age-Related ChangesFlavia Bruna0David Contador1Paulette Conget2Benjamín Erranz3Claudia L. Sossa4Martha L. Arango-Rodríguez5Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, ChileCenter for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, ChileCenter for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, ChileCenter for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, ChileProduction Unity of Advanced Therapy, Fundación Oftalmológica de Santander, Clínica Carlos Ardila Lulle (Foscal Internacional), Universidad Autónoma de Bucaramanga (UNAB), 681004153 Bucaramanga, ColombiaProduction Unity of Advanced Therapy, Fundación Oftalmológica de Santander, Clínica Carlos Ardila Lulle (Foscal Internacional), Universidad Autónoma de Bucaramanga (UNAB), 681004153 Bucaramanga, ColombiaPreclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate whether donor age influences this potential. Two different doses of bone marrow MSCs (BM-MSCs) from young, adult, or old mouse donors or two doses of their acellular derivatives mesenchymal stromal cells (acd-MSCs) were intradermally injected around wounds in the midline of C57BL/6 mice. Every two days, wound healing was macroscopically assessed (wound closure) and microscopically assessed (reepithelialization, dermal-epidermal junction, skin appendage regeneration, granulation tissue, leukocyte infiltration, and density dermal collagen fibers) after 12 days from MSC transplant. Significant differences in the wound closure kinetic, quality, and healing of skin regenerated were observed in lesions which received BM-MSCs from different ages or their acd-MSCs compared to lesions which received vehicle. Nevertheless, our data shows that adult’s BM-MSCs or their acd-MSCs were the most efficient for recovery of most parameters analyzed. Our data suggest that MSC efficacy was negatively affected by donor age, where the treatment with adult’s BM-MSCs or their acd-MSCs in cutaneous wound promotes a better tissue repair/regeneration. This is due to their paracrine factors secretion.http://dx.doi.org/10.1155/2016/1461648 |
| spellingShingle | Flavia Bruna David Contador Paulette Conget Benjamín Erranz Claudia L. Sossa Martha L. Arango-Rodríguez Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes Stem Cells International |
| title | Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes |
| title_full | Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes |
| title_fullStr | Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes |
| title_full_unstemmed | Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes |
| title_short | Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes |
| title_sort | regenerative potential of mesenchymal stromal cells age related changes |
| url | http://dx.doi.org/10.1155/2016/1461648 |
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