Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo
Introduction: Salmonella enterica serovar Typhimurium ST313 is an invasive and phylogenetically distinct lineage present in sub-Saharan Africa. We report the presence of S. Typhimurium ST313 from patients in the Democratic Republic of Congo and Nigeria. Methodology: Eighteen S. Typhimurium ST313 is...
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The Journal of Infection in Developing Countries
2013-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/3328 |
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| author | Pimlapas Leekitcharoenphon Carsten Friis Ea Zankari Christina Aaby Svendsen Lance B. Price Maral Rahmani Ana Herrero-Fresno Kayode Fashae Olivier Vandenberg Frank M. Aarestrup Rene S. Hendriksen |
| author_facet | Pimlapas Leekitcharoenphon Carsten Friis Ea Zankari Christina Aaby Svendsen Lance B. Price Maral Rahmani Ana Herrero-Fresno Kayode Fashae Olivier Vandenberg Frank M. Aarestrup Rene S. Hendriksen |
| author_sort | Pimlapas Leekitcharoenphon |
| collection | DOAJ |
| description | Introduction: Salmonella enterica serovar Typhimurium ST313 is an invasive and phylogenetically distinct lineage present in sub-Saharan Africa. We report the presence of S. Typhimurium ST313 from patients in the Democratic Republic of Congo and Nigeria.
Methodology: Eighteen S. Typhimurium ST313 isolates were characterized by antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). Additionally, six of the isolates were characterized by whole genome sequence typing (WGST). The presence of a putative virulence determinant was examined in 177 Salmonella isolates belonging to 57 different serovars.
Results: All S. Typhimurium ST313 isolates harbored resistant genes encoded by blaTEM1b, catA1, strA/B, sul1, and dfrA1. Additionally, aac(6’)1aa gene was detected. Phylogenetic analyses revealed close genetic relationships among Congolese and Nigerian isolates from both blood and stool. Comparative genomic analyses identified a putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and S. Dublin.
Conclusion: We showed in a limited number of isolates that S. Typhimurium ST313 is a prevalent sequence-type causing gastrointestinal diseases and septicemia in patients from Nigeria and DRC. We found three distinct phylogenetic clusters based on the origin of isolation suggesting some spatial evolution. Comparative genomics showed an interesting putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and invasive S. Dublin.
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| format | Article |
| id | doaj-art-785175bc4ce6462f8b93cb03fe5664d2 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2013-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-785175bc4ce6462f8b93cb03fe5664d22025-08-20T02:16:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802013-10-0171010.3855/jidc.3328Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of CongoPimlapas Leekitcharoenphon0Carsten Friis1Ea Zankari2Christina Aaby Svendsen3Lance B. Price4Maral Rahmani5Ana Herrero-Fresno6Kayode Fashae7Olivier Vandenberg8Frank M. Aarestrup9Rene S. Hendriksen10Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute and Center for Biological Sequence Analysis, Department of System Biology Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby,Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDivision of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDivision of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDivision of Pathogen Genomics, Translational Genomics Research Institute (TGen), Flagstaff, 3051 W. Shamrell Blvd, Ste 106, Flagstaff AZ 86001,Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDivision of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDepartment of Microbiology, University of Ibadan, Ibadan,Infectious Diseases Epidemiological Unit, Public Health School, Université Libre de Bruxelles, Brussels, Route de Lennik, 808 B 1070, Brussels and Department of Microbiology, Saint-Pierre University Hospital, Rue Haute, Hoogstraat 322, Brussels, 1000Division of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkDivision of Bacterial Genomics and Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Food borne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, National Food Institute, Technical University of Denmark, Kemitorvet, Building 204, DK-2800 Kgs. Lyngby, DenmarkIntroduction: Salmonella enterica serovar Typhimurium ST313 is an invasive and phylogenetically distinct lineage present in sub-Saharan Africa. We report the presence of S. Typhimurium ST313 from patients in the Democratic Republic of Congo and Nigeria. Methodology: Eighteen S. Typhimurium ST313 isolates were characterized by antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). Additionally, six of the isolates were characterized by whole genome sequence typing (WGST). The presence of a putative virulence determinant was examined in 177 Salmonella isolates belonging to 57 different serovars. Results: All S. Typhimurium ST313 isolates harbored resistant genes encoded by blaTEM1b, catA1, strA/B, sul1, and dfrA1. Additionally, aac(6’)1aa gene was detected. Phylogenetic analyses revealed close genetic relationships among Congolese and Nigerian isolates from both blood and stool. Comparative genomic analyses identified a putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and S. Dublin. Conclusion: We showed in a limited number of isolates that S. Typhimurium ST313 is a prevalent sequence-type causing gastrointestinal diseases and septicemia in patients from Nigeria and DRC. We found three distinct phylogenetic clusters based on the origin of isolation suggesting some spatial evolution. Comparative genomics showed an interesting putative virulence fragment (ST313-TD) unique to S. Typhimurium ST313 and invasive S. Dublin. https://jidc.org/index.php/journal/article/view/3328Salmonella serovar Typhimurium ST313Sub-Saharan AfricaMLSTwhole genome sequence typingSNP analysisvirulence genes |
| spellingShingle | Pimlapas Leekitcharoenphon Carsten Friis Ea Zankari Christina Aaby Svendsen Lance B. Price Maral Rahmani Ana Herrero-Fresno Kayode Fashae Olivier Vandenberg Frank M. Aarestrup Rene S. Hendriksen Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo Journal of Infection in Developing Countries Salmonella serovar Typhimurium ST313 Sub-Saharan Africa MLST whole genome sequence typing SNP analysis virulence genes |
| title | Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo |
| title_full | Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo |
| title_fullStr | Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo |
| title_full_unstemmed | Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo |
| title_short | Genomics of an emerging clone of Salmonella serovar Typhimurium ST313 from Nigeria and the Democratic Republic of Congo |
| title_sort | genomics of an emerging clone of salmonella serovar typhimurium st313 from nigeria and the democratic republic of congo |
| topic | Salmonella serovar Typhimurium ST313 Sub-Saharan Africa MLST whole genome sequence typing SNP analysis virulence genes |
| url | https://jidc.org/index.php/journal/article/view/3328 |
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