Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis
Abstract Acute pancreatitis (AP) is a prevalent acute gastrointestinal disease, which may be prevented and alleviated by probiotics. Bifidobacterium animalis subsp. lactis BB-12 (BB-12) is a widely studied probiotic strain; however, its specific effects in this context remain unexplored. In this stu...
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SpringerOpen
2025-04-01
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| Online Access: | https://doi.org/10.1186/s13568-025-01867-9 |
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| author | Bingbing Du Ren Yan Xiaoxiang Hu Jing Lou Yixin Zhu Yini Shao Huiyong Jiang Yingying Hao Longxian Lv |
| author_facet | Bingbing Du Ren Yan Xiaoxiang Hu Jing Lou Yixin Zhu Yini Shao Huiyong Jiang Yingying Hao Longxian Lv |
| author_sort | Bingbing Du |
| collection | DOAJ |
| description | Abstract Acute pancreatitis (AP) is a prevalent acute gastrointestinal disease, which may be prevented and alleviated by probiotics. Bifidobacterium animalis subsp. lactis BB-12 (BB-12) is a widely studied probiotic strain; however, its specific effects in this context remain unexplored. In this study, we aimed to investigate the prophylactic and therapeutic effects of BB-12 in AP. Our findings revealed that BB-12 administration via gavage significantly reduced pathological pancreatic damage and serum amylase activity. Microbiome analysis showed that BB-12 treatment significantly increased the relative abundance of Ligilactobacillus and decreased that of Bilophila in the gut microbiota of mice with AP. Transcriptome analysis revealed that BB-12 mitigated the AP-induced dysregulation of several pathways, specifically attenuating the upregulation of the pancreatic secretion and ascorbate and aldarate metabolism pathways while reversing the downregulation of the ribosome, oxidative phosphorylation, and thermogenesis pathways. Spearman’s correlation analysis revealed a positive correlation between the abundances of Bilophila and ASF356 and serum amylase activity. Furthermore, the abundances of Bilophila and ASF356 were significantly correlated with BB-12-regulated pancreatic genes and were predominantly enriched in the ribosome pathway. In conclusion, BB-12 pretreatment alleviated AP, likely by regulating the abundance of intestinal Lactobacillus, Bilophila, and ASF356, as well as the pancreatic secretion, ascorbate and aldarate metabolism, oxidative phosphorylation, ribosome, and thermogenesis pathways. |
| format | Article |
| id | doaj-art-784cd6cb988c4d7a88937cb33be636df |
| institution | OA Journals |
| issn | 2191-0855 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
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| spelling | doaj-art-784cd6cb988c4d7a88937cb33be636df2025-08-20T01:54:30ZengSpringerOpenAMB Express2191-08552025-04-0115111110.1186/s13568-025-01867-9Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitisBingbing Du0Ren Yan1Xiaoxiang Hu2Jing Lou3Yixin Zhu4Yini Shao5Huiyong Jiang6Yingying Hao7Longxian Lv8Shandong First Medical University and Shandong Academy of Medical SciencesState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityJinan Microecological Biomedicine Shandong LaboratoryState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityDepartment of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan Microecological Biomedicine Shandong LaboratoryAbstract Acute pancreatitis (AP) is a prevalent acute gastrointestinal disease, which may be prevented and alleviated by probiotics. Bifidobacterium animalis subsp. lactis BB-12 (BB-12) is a widely studied probiotic strain; however, its specific effects in this context remain unexplored. In this study, we aimed to investigate the prophylactic and therapeutic effects of BB-12 in AP. Our findings revealed that BB-12 administration via gavage significantly reduced pathological pancreatic damage and serum amylase activity. Microbiome analysis showed that BB-12 treatment significantly increased the relative abundance of Ligilactobacillus and decreased that of Bilophila in the gut microbiota of mice with AP. Transcriptome analysis revealed that BB-12 mitigated the AP-induced dysregulation of several pathways, specifically attenuating the upregulation of the pancreatic secretion and ascorbate and aldarate metabolism pathways while reversing the downregulation of the ribosome, oxidative phosphorylation, and thermogenesis pathways. Spearman’s correlation analysis revealed a positive correlation between the abundances of Bilophila and ASF356 and serum amylase activity. Furthermore, the abundances of Bilophila and ASF356 were significantly correlated with BB-12-regulated pancreatic genes and were predominantly enriched in the ribosome pathway. In conclusion, BB-12 pretreatment alleviated AP, likely by regulating the abundance of intestinal Lactobacillus, Bilophila, and ASF356, as well as the pancreatic secretion, ascorbate and aldarate metabolism, oxidative phosphorylation, ribosome, and thermogenesis pathways.https://doi.org/10.1186/s13568-025-01867-9ProbioticAcute pancreatitisGut microbiotaBifidobacterium animalis subsp. lactis BB-12 |
| spellingShingle | Bingbing Du Ren Yan Xiaoxiang Hu Jing Lou Yixin Zhu Yini Shao Huiyong Jiang Yingying Hao Longxian Lv Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis AMB Express Probiotic Acute pancreatitis Gut microbiota Bifidobacterium animalis subsp. lactis BB-12 |
| title | Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis |
| title_full | Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis |
| title_fullStr | Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis |
| title_full_unstemmed | Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis |
| title_short | Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis |
| title_sort | role of bifidobacterium animalis subsp lactis bb 12 in mice with acute pancreatitis |
| topic | Probiotic Acute pancreatitis Gut microbiota Bifidobacterium animalis subsp. lactis BB-12 |
| url | https://doi.org/10.1186/s13568-025-01867-9 |
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