Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii
Glycogen is a primary cellular energy store in numerous eukaryotes. Its biosynthesis is a main strategy to cope with forthcoming starvation. During starvation, glycogen is processed in the cytosol or delivered for degradation to animal lysosomes or yeast vacuoles by macroautophagy (hereafter autopha...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Autophagy Reports |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/27694127.2025.2467454 |
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| author | Nimna V. Wijewantha Taras Y. Nazarko |
| author_facet | Nimna V. Wijewantha Taras Y. Nazarko |
| author_sort | Nimna V. Wijewantha |
| collection | DOAJ |
| description | Glycogen is a primary cellular energy store in numerous eukaryotes. Its biosynthesis is a main strategy to cope with forthcoming starvation. During starvation, glycogen is processed in the cytosol or delivered for degradation to animal lysosomes or yeast vacuoles by macroautophagy (hereafter autophagy). However, the mechanism of glycogen autophagy is poorly understood, especially in the heart and skeletal muscles that suffer from the lysosomal glycogen accumulation in Pompe disease. We recently developed the Komagataella phaffii yeast as a simple model to study glycogen autophagy and found that this pathway proceeds non-selectively. However, studies in Saccharomyces cerevisiae proposed glycogen as a non-preferred cargo of bulk autophagy. In our latest study with new fluorescent reporters for glycogen, we clarified cargo properties of K. phaffii glycogen. Both homologous and heterologous markers of glycogen are delivered to the vacuole and degraded with efficiencies that are independent of glycogen, suggesting that glycogen is a neutral cargo of bulk autophagy. This work provides insights into the evolutionary diversity of glycogen autophagy in yeasts with implications for understanding this process in complex eukaryotes. |
| format | Article |
| id | doaj-art-784c2376cae6446989b8b8b042d49f6d |
| institution | DOAJ |
| issn | 2769-4127 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autophagy Reports |
| spelling | doaj-art-784c2376cae6446989b8b8b042d49f6d2025-08-20T03:05:52ZengTaylor & Francis GroupAutophagy Reports2769-41272025-12-014110.1080/27694127.2025.2467454Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffiiNimna V. Wijewantha0Taras Y. Nazarko1Department of Biology, Georgia State University, Atlanta, USADepartment of Biology, Georgia State University, Atlanta, USAGlycogen is a primary cellular energy store in numerous eukaryotes. Its biosynthesis is a main strategy to cope with forthcoming starvation. During starvation, glycogen is processed in the cytosol or delivered for degradation to animal lysosomes or yeast vacuoles by macroautophagy (hereafter autophagy). However, the mechanism of glycogen autophagy is poorly understood, especially in the heart and skeletal muscles that suffer from the lysosomal glycogen accumulation in Pompe disease. We recently developed the Komagataella phaffii yeast as a simple model to study glycogen autophagy and found that this pathway proceeds non-selectively. However, studies in Saccharomyces cerevisiae proposed glycogen as a non-preferred cargo of bulk autophagy. In our latest study with new fluorescent reporters for glycogen, we clarified cargo properties of K. phaffii glycogen. Both homologous and heterologous markers of glycogen are delivered to the vacuole and degraded with efficiencies that are independent of glycogen, suggesting that glycogen is a neutral cargo of bulk autophagy. This work provides insights into the evolutionary diversity of glycogen autophagy in yeasts with implications for understanding this process in complex eukaryotes.https://www.tandfonline.com/doi/10.1080/27694127.2025.2467454CBM20glycogen granulesglycogen synthaseglycophagyyeast |
| spellingShingle | Nimna V. Wijewantha Taras Y. Nazarko Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii Autophagy Reports CBM20 glycogen granules glycogen synthase glycophagy yeast |
| title | Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii |
| title_full | Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii |
| title_fullStr | Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii |
| title_full_unstemmed | Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii |
| title_short | Glycogen is a neutral cargo of bulk autophagy in Komagataella phaffii |
| title_sort | glycogen is a neutral cargo of bulk autophagy in komagataella phaffii |
| topic | CBM20 glycogen granules glycogen synthase glycophagy yeast |
| url | https://www.tandfonline.com/doi/10.1080/27694127.2025.2467454 |
| work_keys_str_mv | AT nimnavwijewantha glycogenisaneutralcargoofbulkautophagyinkomagataellaphaffii AT tarasynazarko glycogenisaneutralcargoofbulkautophagyinkomagataellaphaffii |