Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections
CRISPR-Cas13 is a groundbreaking RNA-targeting tool that has emerged as a promising solution for diagnosing and treating RNA viruses, including SARS-CoV-2, influenza, and HIV. By leveraging the CRISPR-Cas13 system, researchers can precisely target and cleave single-stranded RNA, thereby disrupting v...
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| Format: | Article |
| Language: | English |
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Ivano-Frankivsk National Medical University
2025-05-01
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| Series: | Galician Medical Journal |
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| Online Access: | https://ifnmujournal.com/gmj/article/view/2071 |
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| author | Sharanagouda S. Patil Krishna Prasanth Baalann Danti Joseph Sriram N Nirmali Gogoi |
| author_facet | Sharanagouda S. Patil Krishna Prasanth Baalann Danti Joseph Sriram N Nirmali Gogoi |
| author_sort | Sharanagouda S. Patil |
| collection | DOAJ |
| description | CRISPR-Cas13 is a groundbreaking RNA-targeting tool that has emerged as a promising solution for diagnosing and treating RNA viruses, including SARS-CoV-2, influenza, and HIV. By leveraging the CRISPR-Cas13 system, researchers can precisely target and cleave single-stranded RNA, thereby disrupting viral replication and enabling rapid molecular diagnostics. The CRISPR-Cas13 system boasts several advantages over traditional antiviral strategies, including exceptional specificity and sensitivity, rapid diagnostic capabilities, and potential for broad-spectrum antiviral activity. This technology has far-reaching implications for diagnosing and treating RNA viral infections, which pose significant global health threats due to their high mutation rates and resistance to conventional treatments. While CRISPR-Cas13 holds tremendous promise, there are challenges to be addressed, such as optimizing delivery methods to ensure efficient targeting of viral RNA and minimizing off-target effects. Experiments are also needed to fully elucidate the mechanism of RNA degradation by CRISPR-Cas13 and explore its use in treating diseases. Despite such limitations, promising prospects in practical applications, such as in the SHERLOCK and PAC-MAN systems, have already been demonstrated by CRISPR-Cas13. These innovative technologies have harnessed the power of CRISPR-Cas13 to detect and treat RNA viral infections, paving the way for developing novel diagnostic and therapeutic strategies. |
| format | Article |
| id | doaj-art-7847fdcaabd44a04b9873898b3ab7dac |
| institution | OA Journals |
| issn | 2414-1518 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Ivano-Frankivsk National Medical University |
| record_format | Article |
| series | Galician Medical Journal |
| spelling | doaj-art-7847fdcaabd44a04b9873898b3ab7dac2025-08-20T02:38:29ZengIvano-Frankivsk National Medical UniversityGalician Medical Journal2414-15182025-05-0132210.21802/e-GMJ2025-A132071Therapeutic Potential of CRISPR-Cas13 for RNA Virus InfectionsSharanagouda S. Patil0Krishna Prasanth Baalann1Danti Joseph2Sriram N3Nirmali Gogoi4ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, Karnataka, IndiaBharath Institute of Higher Education and Research, Chennai, IndiaSree Balaji Medical College and Hospital, Chennai, IndiaUsha College Of Pharmacy, Dhadkidih, Jharkhand, IndiaFaculty of Nursing, Assam Down Town University, Guwahati, Assam, IndiaCRISPR-Cas13 is a groundbreaking RNA-targeting tool that has emerged as a promising solution for diagnosing and treating RNA viruses, including SARS-CoV-2, influenza, and HIV. By leveraging the CRISPR-Cas13 system, researchers can precisely target and cleave single-stranded RNA, thereby disrupting viral replication and enabling rapid molecular diagnostics. The CRISPR-Cas13 system boasts several advantages over traditional antiviral strategies, including exceptional specificity and sensitivity, rapid diagnostic capabilities, and potential for broad-spectrum antiviral activity. This technology has far-reaching implications for diagnosing and treating RNA viral infections, which pose significant global health threats due to their high mutation rates and resistance to conventional treatments. While CRISPR-Cas13 holds tremendous promise, there are challenges to be addressed, such as optimizing delivery methods to ensure efficient targeting of viral RNA and minimizing off-target effects. Experiments are also needed to fully elucidate the mechanism of RNA degradation by CRISPR-Cas13 and explore its use in treating diseases. Despite such limitations, promising prospects in practical applications, such as in the SHERLOCK and PAC-MAN systems, have already been demonstrated by CRISPR-Cas13. These innovative technologies have harnessed the power of CRISPR-Cas13 to detect and treat RNA viral infections, paving the way for developing novel diagnostic and therapeutic strategies.https://ifnmujournal.com/gmj/article/view/2071crispr-cas13rna virusesdiagnosticsantiviral therapygenome editingsars-cov-2pac-mansherlockmolecular medicine |
| spellingShingle | Sharanagouda S. Patil Krishna Prasanth Baalann Danti Joseph Sriram N Nirmali Gogoi Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections Galician Medical Journal crispr-cas13 rna viruses diagnostics antiviral therapy genome editing sars-cov-2 pac-man sherlock molecular medicine |
| title | Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections |
| title_full | Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections |
| title_fullStr | Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections |
| title_full_unstemmed | Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections |
| title_short | Therapeutic Potential of CRISPR-Cas13 for RNA Virus Infections |
| title_sort | therapeutic potential of crispr cas13 for rna virus infections |
| topic | crispr-cas13 rna viruses diagnostics antiviral therapy genome editing sars-cov-2 pac-man sherlock molecular medicine |
| url | https://ifnmujournal.com/gmj/article/view/2071 |
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