Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae.
Klebsiella pneumoniae is a typical opportunistic pathogen that exhibits multiple virulence factors and antibiotic resistance conditioning mechanisms. Carbapenemases are enzymes that help bacteria to exhibit the strongest resistance against antibiotics. Therefore, in routine microbiological diagnoses...
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| Format: | Article |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0318852 |
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| author | Agata Pruss Alicja Skierska Paweł Kwiatkowski Helena Masiuk Joanna Jursa-Kulesza Stefania Giedrys-Kalemba Iwona Bilska Monika Sienkiewicz Maria V Melnyk Barbara Dołęgowska |
| author_facet | Agata Pruss Alicja Skierska Paweł Kwiatkowski Helena Masiuk Joanna Jursa-Kulesza Stefania Giedrys-Kalemba Iwona Bilska Monika Sienkiewicz Maria V Melnyk Barbara Dołęgowska |
| author_sort | Agata Pruss |
| collection | DOAJ |
| description | Klebsiella pneumoniae is a typical opportunistic pathogen that exhibits multiple virulence factors and antibiotic resistance conditioning mechanisms. Carbapenemases are enzymes that help bacteria to exhibit the strongest resistance against antibiotics. Therefore, in routine microbiological diagnoses, it is crucial to confirm antibiotic-resistant strains, including carbapenemase-producing bacteria strains, isolated from patients. Two types of tests play an important role here: phenotypicand molecular diagnostic methods. The latter complement phenotypic tests and a mandatory procedure to confirm the detection of carbapenemases. This study aimed to evaluate the usefulness and effectiveness of tests and methods used to identify and confirm the ability of clinical K. pneumoniae strains to produce carbapenemases. The production of carbapenemases was assessed using phenotypic and genetic methods. The strains tested showed complete resistance to most beta-lactams and varying sensitivity to drugs from the quinolone carbapenem group and aminoglycosides. Among the most commonly produced carbapenemases were the metallo-beta-lactamase (NDM) family. The most accurate phenotypic method for detecting carbapenemases was the NG CARBA-5 assay, and the PCR method confirmed these results. Notably, a few inconclusive results were obtained for NDM-positive and VIM-positive strains when the disk diffusion method and CIM test were used. Further, the Carba tube assay and the RAPIDEC CARBA NP assay produced questionable results for the OXA-48 strain group. This group also generated false-negative results on Carba's CHROM ID medium. |
| format | Article |
| id | doaj-art-7844c251d3d844039bd625684ffc3beb |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7844c251d3d844039bd625684ffc3beb2025-02-12T05:31:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031885210.1371/journal.pone.0318852Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae.Agata PrussAlicja SkierskaPaweł KwiatkowskiHelena MasiukJoanna Jursa-KuleszaStefania Giedrys-KalembaIwona BilskaMonika SienkiewiczMaria V MelnykBarbara DołęgowskaKlebsiella pneumoniae is a typical opportunistic pathogen that exhibits multiple virulence factors and antibiotic resistance conditioning mechanisms. Carbapenemases are enzymes that help bacteria to exhibit the strongest resistance against antibiotics. Therefore, in routine microbiological diagnoses, it is crucial to confirm antibiotic-resistant strains, including carbapenemase-producing bacteria strains, isolated from patients. Two types of tests play an important role here: phenotypicand molecular diagnostic methods. The latter complement phenotypic tests and a mandatory procedure to confirm the detection of carbapenemases. This study aimed to evaluate the usefulness and effectiveness of tests and methods used to identify and confirm the ability of clinical K. pneumoniae strains to produce carbapenemases. The production of carbapenemases was assessed using phenotypic and genetic methods. The strains tested showed complete resistance to most beta-lactams and varying sensitivity to drugs from the quinolone carbapenem group and aminoglycosides. Among the most commonly produced carbapenemases were the metallo-beta-lactamase (NDM) family. The most accurate phenotypic method for detecting carbapenemases was the NG CARBA-5 assay, and the PCR method confirmed these results. Notably, a few inconclusive results were obtained for NDM-positive and VIM-positive strains when the disk diffusion method and CIM test were used. Further, the Carba tube assay and the RAPIDEC CARBA NP assay produced questionable results for the OXA-48 strain group. This group also generated false-negative results on Carba's CHROM ID medium.https://doi.org/10.1371/journal.pone.0318852 |
| spellingShingle | Agata Pruss Alicja Skierska Paweł Kwiatkowski Helena Masiuk Joanna Jursa-Kulesza Stefania Giedrys-Kalemba Iwona Bilska Monika Sienkiewicz Maria V Melnyk Barbara Dołęgowska Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. PLoS ONE |
| title | Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. |
| title_full | Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. |
| title_fullStr | Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. |
| title_full_unstemmed | Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. |
| title_short | Comparative analysis of selected methods of carbapenemase determination among clinical Klebsiella pneumoniae. |
| title_sort | comparative analysis of selected methods of carbapenemase determination among clinical klebsiella pneumoniae |
| url | https://doi.org/10.1371/journal.pone.0318852 |
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