An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions

Abstract Background Mesenchymal stem cells have shown promise in many areas of regenerative medicine due to the anti-inflammatory and pro-regenerative effects of the secreted factors. However, successful delivery remains problematic, particularly for delivery to areas such as the brain. Spray delive...

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Main Authors: Ami Nash, I-Ning Lee, Graeme Fox, James Phillips, Lisa J White, Maria Marlow
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Stem Cell Research & Therapy
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Online Access:https://doi.org/10.1186/s13287-025-04331-4
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author Ami Nash
I-Ning Lee
Graeme Fox
James Phillips
Lisa J White
Maria Marlow
author_facet Ami Nash
I-Ning Lee
Graeme Fox
James Phillips
Lisa J White
Maria Marlow
author_sort Ami Nash
collection DOAJ
description Abstract Background Mesenchymal stem cells have shown promise in many areas of regenerative medicine due to the anti-inflammatory and pro-regenerative effects of the secreted factors. However, successful delivery remains problematic, particularly for delivery to areas such as the brain. Spray delivery is a method investigated in wound care and lung injury, which may be applicable for brain delivery to patients already requiring surgery. To retain therapeutic mesenchymal stem cells at the delivery site, biomaterials can be employed; pectin is a biocompatible, sprayable, and mucoadhesive material, which could prove suitable for spray delivery of cells for therapeutic uses. Methods The biocompatibility of four grades of low-methyl pectin gelled by addition of calcium was assessed using SH-SY5Y cells. After, mesenchymal stem cells were suspended within the four different grades of low-methyl pectin solutions and sprayed using a syringe-driven spray device. The suitability was then assessed by cell viability testing, flow cytometry to test for surface markers, and differential gene expression studies to understand the effects of both the pectin and the spraying process on the gene expression of the cells. Results All four grades of low-methyl pectin were biocompatible with SH-SY5Y cells. The syringe-driven spray device delivered human mesenchymal stem cells to well plates with high viability, and suspending these cells in pectin solutions for spraying did not negatively affect the viability. The grade of pectin named CU-701 was the best grade based on results of the flow cytometry, whereby the surface marker expression was not altered from the control cells. The RNA sequencing showing the differential expression showed that the process of spraying the cells did not alter gene expression compared to the control, however the pectin, and the presence of calcium used to induce gelation of the pectin, did lead to altered gene expression in cells. Conclusion Spraying is a suitable delivery method for the mesenchymal stem cells, showing no detrimental effect on the cells. Pectin shows little effect on the viability of the cells, however the use of calcium to gel the pectin appears to affect the expression of several genes.
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spelling doaj-art-7841fe3179fd42ca97382550a4e7d0392025-08-20T03:53:57ZengBMCStem Cell Research & Therapy1757-65122025-05-0116111110.1186/s13287-025-04331-4An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutionsAmi Nash0I-Ning Lee1Graeme Fox2James Phillips3Lisa J White4Maria Marlow5School of Pharmacy, University of NottinghamSchool of Pharmacy, University of NottinghamDeep Seq, Centre for Genetics and Genomics, University of Nottingham, Queen’s Medical CentreDepartment of Pharmacology, School of Pharmacy, University College LondonSchool of Pharmacy, University of NottinghamSchool of Pharmacy, University of NottinghamAbstract Background Mesenchymal stem cells have shown promise in many areas of regenerative medicine due to the anti-inflammatory and pro-regenerative effects of the secreted factors. However, successful delivery remains problematic, particularly for delivery to areas such as the brain. Spray delivery is a method investigated in wound care and lung injury, which may be applicable for brain delivery to patients already requiring surgery. To retain therapeutic mesenchymal stem cells at the delivery site, biomaterials can be employed; pectin is a biocompatible, sprayable, and mucoadhesive material, which could prove suitable for spray delivery of cells for therapeutic uses. Methods The biocompatibility of four grades of low-methyl pectin gelled by addition of calcium was assessed using SH-SY5Y cells. After, mesenchymal stem cells were suspended within the four different grades of low-methyl pectin solutions and sprayed using a syringe-driven spray device. The suitability was then assessed by cell viability testing, flow cytometry to test for surface markers, and differential gene expression studies to understand the effects of both the pectin and the spraying process on the gene expression of the cells. Results All four grades of low-methyl pectin were biocompatible with SH-SY5Y cells. The syringe-driven spray device delivered human mesenchymal stem cells to well plates with high viability, and suspending these cells in pectin solutions for spraying did not negatively affect the viability. The grade of pectin named CU-701 was the best grade based on results of the flow cytometry, whereby the surface marker expression was not altered from the control cells. The RNA sequencing showing the differential expression showed that the process of spraying the cells did not alter gene expression compared to the control, however the pectin, and the presence of calcium used to induce gelation of the pectin, did lead to altered gene expression in cells. Conclusion Spraying is a suitable delivery method for the mesenchymal stem cells, showing no detrimental effect on the cells. Pectin shows little effect on the viability of the cells, however the use of calcium to gel the pectin appears to affect the expression of several genes.https://doi.org/10.1186/s13287-025-04331-4Human mesenchymal stem cellsSprayingPectin
spellingShingle Ami Nash
I-Ning Lee
Graeme Fox
James Phillips
Lisa J White
Maria Marlow
An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
Stem Cell Research & Therapy
Human mesenchymal stem cells
Spraying
Pectin
title An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
title_full An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
title_fullStr An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
title_full_unstemmed An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
title_short An evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low-methyl pectin solutions
title_sort evaluation of spraying as a delivery method for human mesenchymal stem cells suspended in low methyl pectin solutions
topic Human mesenchymal stem cells
Spraying
Pectin
url https://doi.org/10.1186/s13287-025-04331-4
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