Vesiculation from Pseudomonas aeruginosa under SOS
Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/402919 |
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| author | Reshma Maredia Navya Devineni Peter Lentz Shatha F. Dallo JiehJuen Yu Neal Guentzel James Chambers Bernard Arulanandam William E. Haskins Tao Weitao |
| author_facet | Reshma Maredia Navya Devineni Peter Lentz Shatha F. Dallo JiehJuen Yu Neal Guentzel James Chambers Bernard Arulanandam William E. Haskins Tao Weitao |
| author_sort | Reshma Maredia |
| collection | DOAJ |
| description | Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (lexAN) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity. |
| format | Article |
| id | doaj-art-783f09e06e5b45cd8dbf8a8acd67cc5e |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-783f09e06e5b45cd8dbf8a8acd67cc5e2025-08-20T03:35:11ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/402919402919Vesiculation from Pseudomonas aeruginosa under SOSReshma Maredia0Navya Devineni1Peter Lentz2Shatha F. Dallo3JiehJuen Yu4Neal Guentzel5James Chambers6Bernard Arulanandam7William E. Haskins8Tao Weitao9Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USAPediatric Biochemistry Laboratory, The University of Texas at San Antonio, San Antonio, TX 78249, USADepartment of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USABacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (lexAN) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.http://dx.doi.org/10.1100/2012/402919 |
| spellingShingle | Reshma Maredia Navya Devineni Peter Lentz Shatha F. Dallo JiehJuen Yu Neal Guentzel James Chambers Bernard Arulanandam William E. Haskins Tao Weitao Vesiculation from Pseudomonas aeruginosa under SOS The Scientific World Journal |
| title | Vesiculation from Pseudomonas aeruginosa under SOS |
| title_full | Vesiculation from Pseudomonas aeruginosa under SOS |
| title_fullStr | Vesiculation from Pseudomonas aeruginosa under SOS |
| title_full_unstemmed | Vesiculation from Pseudomonas aeruginosa under SOS |
| title_short | Vesiculation from Pseudomonas aeruginosa under SOS |
| title_sort | vesiculation from pseudomonas aeruginosa under sos |
| url | http://dx.doi.org/10.1100/2012/402919 |
| work_keys_str_mv | AT reshmamaredia vesiculationfrompseudomonasaeruginosaundersos AT navyadevineni vesiculationfrompseudomonasaeruginosaundersos AT peterlentz vesiculationfrompseudomonasaeruginosaundersos AT shathafdallo vesiculationfrompseudomonasaeruginosaundersos AT jiehjuenyu vesiculationfrompseudomonasaeruginosaundersos AT nealguentzel vesiculationfrompseudomonasaeruginosaundersos AT jameschambers vesiculationfrompseudomonasaeruginosaundersos AT bernardarulanandam vesiculationfrompseudomonasaeruginosaundersos AT williamehaskins vesiculationfrompseudomonasaeruginosaundersos AT taoweitao vesiculationfrompseudomonasaeruginosaundersos |