Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity

Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity

Abstract Background Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time PCR (qPCR) assays, but they require...

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Bibliographic Details
Main Authors: Zijin Zhao, Yanbo You, Shaowei Hua, Xinxin Shen, Lingjun Li, Xuejun Ma
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:iLabmed
Subjects:
EvaGreen dye
Listeria monocytogenes
melt curve
nucleotide analogs
raw milk
recombinase aided amplification
Online Access:https://doi.org/10.1002/ila2.51
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