Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation
Left-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demon...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/1848605 |
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| _version_ | 1849410265397854208 |
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| author | Kathryn E. Worley Amanda S. Chin Leo Q. Wan |
| author_facet | Kathryn E. Worley Amanda S. Chin Leo Q. Wan |
| author_sort | Kathryn E. Worley |
| collection | DOAJ |
| description | Left-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demonstrate that inherent cellular chirality is observable in the cells of early embryonic stages using a 3D Matrigel bilayer system. Differentiation of human embryonic stem cells to three lineages corresponding to heart, intestine, and neural tissues demonstrates phenotype-specific inherent chiral biases, complementing the current knowledge regarding organ development. The existence of inherent cellular chirality early in development and its correlation with organ asymmetry implicate cell chirality as a possible regulator in LR symmetry breaking. |
| format | Article |
| id | doaj-art-7822818caaf040ab83be425658cb1cda |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-7822818caaf040ab83be425658cb1cda2025-08-20T03:35:11ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/18486051848605Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during DifferentiationKathryn E. Worley0Amanda S. Chin1Leo Q. Wan2Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180, USADepartment of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180, USADepartment of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy NY 12180, USALeft-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demonstrate that inherent cellular chirality is observable in the cells of early embryonic stages using a 3D Matrigel bilayer system. Differentiation of human embryonic stem cells to three lineages corresponding to heart, intestine, and neural tissues demonstrates phenotype-specific inherent chiral biases, complementing the current knowledge regarding organ development. The existence of inherent cellular chirality early in development and its correlation with organ asymmetry implicate cell chirality as a possible regulator in LR symmetry breaking.http://dx.doi.org/10.1155/2018/1848605 |
| spellingShingle | Kathryn E. Worley Amanda S. Chin Leo Q. Wan Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation Stem Cells International |
| title | Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation |
| title_full | Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation |
| title_fullStr | Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation |
| title_full_unstemmed | Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation |
| title_short | Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation |
| title_sort | lineage specific chiral biases of human embryonic stem cells during differentiation |
| url | http://dx.doi.org/10.1155/2018/1848605 |
| work_keys_str_mv | AT kathryneworley lineagespecificchiralbiasesofhumanembryonicstemcellsduringdifferentiation AT amandaschin lineagespecificchiralbiasesofhumanembryonicstemcellsduringdifferentiation AT leoqwan lineagespecificchiralbiasesofhumanembryonicstemcellsduringdifferentiation |