Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots
The plant hormones cytokinins are a class of heterogeneous active compounds that control multiple aspects of development and physiology. Among cytokinins, trans-zeatin (<i>tZ</i>), the most abundant cytokinin, has been extensively studied in relation to its effects on development, and it...
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MDPI AG
2025-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/10/1501 |
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| author | Federico Vinciarelli Mirko De Vivo Alessio Terenzi Francesca Cazzaniga Samuele Amati Pierpaolo Damato Elena Salvi Marta Del Bianco Riccardo Di Mambro Paolo Costantino Sabrina Sabatini Raffaele Dello Ioio |
| author_facet | Federico Vinciarelli Mirko De Vivo Alessio Terenzi Francesca Cazzaniga Samuele Amati Pierpaolo Damato Elena Salvi Marta Del Bianco Riccardo Di Mambro Paolo Costantino Sabrina Sabatini Raffaele Dello Ioio |
| author_sort | Federico Vinciarelli |
| collection | DOAJ |
| description | The plant hormones cytokinins are a class of heterogeneous active compounds that control multiple aspects of development and physiology. Among cytokinins, trans-zeatin (<i>tZ</i>), the most abundant cytokinin, has been extensively studied in relation to its effects on development, and it plays a key role in promoting cell differentiation. In analogy with <i>tZ</i>, here we demonstrate that dihydrozeatin (DHZ) controls (root) development by promoting cell differentiation. By means of pharmacological and genetic analysis, we demonstrate that DHZ is specifically and uniquely perceived by the histidine kinase (HK) receptor AHK3, and that this interaction is sufficient to promote cell differentiation in the root meristem via activation of the transcription factors <i>ARABIDOPSIS</i> RESPONSE REGULATOR 1, 12, and 11. We also show that DHZ and <i>tZ</i> activity might be conserved among plants. Our results support the idea that different types of cytokinins act via specific receptors to exert their roles and suggest new approaches to study their activity in differentiation. |
| format | Article |
| id | doaj-art-781decfa101b490d9c3d979372a8ca51 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-781decfa101b490d9c3d979372a8ca512025-08-20T03:12:04ZengMDPI AGPlants2223-77472025-05-011410150110.3390/plants14101501Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> RootsFederico Vinciarelli0Mirko De Vivo1Alessio Terenzi2Francesca Cazzaniga3Samuele Amati4Pierpaolo Damato5Elena Salvi6Marta Del Bianco7Riccardo Di Mambro8Paolo Costantino9Sabrina Sabatini10Raffaele Dello Ioio11Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology, Università di Pisa (UNIPI), 56126 Pisa, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology, Università di Pisa (UNIPI), 56126 Pisa, ItalyAgenzia Spaziale Italiana (ASI), 00133 Rome, ItalyDepartment of Biology, Università di Pisa (UNIPI), 56126 Pisa, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, 00185 Rome, ItalyThe plant hormones cytokinins are a class of heterogeneous active compounds that control multiple aspects of development and physiology. Among cytokinins, trans-zeatin (<i>tZ</i>), the most abundant cytokinin, has been extensively studied in relation to its effects on development, and it plays a key role in promoting cell differentiation. In analogy with <i>tZ</i>, here we demonstrate that dihydrozeatin (DHZ) controls (root) development by promoting cell differentiation. By means of pharmacological and genetic analysis, we demonstrate that DHZ is specifically and uniquely perceived by the histidine kinase (HK) receptor AHK3, and that this interaction is sufficient to promote cell differentiation in the root meristem via activation of the transcription factors <i>ARABIDOPSIS</i> RESPONSE REGULATOR 1, 12, and 11. We also show that DHZ and <i>tZ</i> activity might be conserved among plants. Our results support the idea that different types of cytokinins act via specific receptors to exert their roles and suggest new approaches to study their activity in differentiation.https://www.mdpi.com/2223-7747/14/10/1501cytokininsroot developmentroot meristemdihydrozeatinhormonal regulation |
| spellingShingle | Federico Vinciarelli Mirko De Vivo Alessio Terenzi Francesca Cazzaniga Samuele Amati Pierpaolo Damato Elena Salvi Marta Del Bianco Riccardo Di Mambro Paolo Costantino Sabrina Sabatini Raffaele Dello Ioio Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots Plants cytokinins root development root meristem dihydrozeatin hormonal regulation |
| title | Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots |
| title_full | Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots |
| title_fullStr | Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots |
| title_full_unstemmed | Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots |
| title_short | Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity in <i>Arabidopsis</i> Roots |
| title_sort | identification of a specific role of dihydrozeatin in the regulation of the cell differentiation activity in i arabidopsis i roots |
| topic | cytokinins root development root meristem dihydrozeatin hormonal regulation |
| url | https://www.mdpi.com/2223-7747/14/10/1501 |
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