Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering
Poly(vinyl alcohol) (PVA) was grafted to chitosan to form a porous scaffold. The PVA-g-chitosan 3D scaffold was then observed by Fourier transform infrared spectroscopy (FT-IR). The water absorbency of PVA-g-chitosan was increased 370% by grafting. Scanning electron microscope (SEM) observations of...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2015/935305 |
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| _version_ | 1849410301043146752 |
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| author | Wen-Chuan Hsieh Jiun-Jia Liau Yi-Jhong Li |
| author_facet | Wen-Chuan Hsieh Jiun-Jia Liau Yi-Jhong Li |
| author_sort | Wen-Chuan Hsieh |
| collection | DOAJ |
| description | Poly(vinyl alcohol) (PVA) was grafted to chitosan to form a porous scaffold. The PVA-g-chitosan 3D scaffold was then observed by Fourier transform infrared spectroscopy (FT-IR). The water absorbency of PVA-g-chitosan was increased 370% by grafting. Scanning electron microscope (SEM) observations of the material revealed that the 3D scaffold is highly porous when formed using a homogenizer at 300 rpm. Compression testing demonstrated that as the amount of chitosan increases, the strength of the 3D scaffold strength reached showed that, by increasing the amount of chitosan, the strength of the 3D scaffold could be increased to 16 × 10−1 MPa. Over 35 days of enzymatic degradation, the 3D scaffold was degraded by various enzymes at rates of up to 10%. In vitro tests showed good cell proliferation and growth in the 3D scaffold. |
| format | Article |
| id | doaj-art-7811ec2cd0d84ec19031d650e870dcf3 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-7811ec2cd0d84ec19031d650e870dcf32025-08-20T03:35:10ZengWileyInternational Journal of Polymer Science1687-94221687-94302015-01-01201510.1155/2015/935305935305Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue EngineeringWen-Chuan Hsieh0Jiun-Jia Liau1Yi-Jhong Li2Department of Biological Science and Technology, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Biological Science and Technology, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Biological Science and Technology, I-Shou University, Kaohsiung 82445, TaiwanPoly(vinyl alcohol) (PVA) was grafted to chitosan to form a porous scaffold. The PVA-g-chitosan 3D scaffold was then observed by Fourier transform infrared spectroscopy (FT-IR). The water absorbency of PVA-g-chitosan was increased 370% by grafting. Scanning electron microscope (SEM) observations of the material revealed that the 3D scaffold is highly porous when formed using a homogenizer at 300 rpm. Compression testing demonstrated that as the amount of chitosan increases, the strength of the 3D scaffold strength reached showed that, by increasing the amount of chitosan, the strength of the 3D scaffold could be increased to 16 × 10−1 MPa. Over 35 days of enzymatic degradation, the 3D scaffold was degraded by various enzymes at rates of up to 10%. In vitro tests showed good cell proliferation and growth in the 3D scaffold.http://dx.doi.org/10.1155/2015/935305 |
| spellingShingle | Wen-Chuan Hsieh Jiun-Jia Liau Yi-Jhong Li Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering International Journal of Polymer Science |
| title | Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering |
| title_full | Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering |
| title_fullStr | Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering |
| title_full_unstemmed | Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering |
| title_short | Characterization and Cell Culture of a Grafted Chitosan Scaffold for Tissue Engineering |
| title_sort | characterization and cell culture of a grafted chitosan scaffold for tissue engineering |
| url | http://dx.doi.org/10.1155/2015/935305 |
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