Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily

The fungal kingdom, with an estimated five million species, has undergone extensive diversification over the past billion years and now occupies a wide array of ecological niches from terrestrial to aquatic ecosystems. To thrive in such diverse environments, fungi must exhibit finely tuned physiolog...

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Main Authors: Jean-Stéphane Venisse, Gisèle Bronner, Mouadh Saadaoui, Patricia Roeckel-Drevet, Mohamed Faize, Boris Fumanal
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/11/7/543
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author Jean-Stéphane Venisse
Gisèle Bronner
Mouadh Saadaoui
Patricia Roeckel-Drevet
Mohamed Faize
Boris Fumanal
author_facet Jean-Stéphane Venisse
Gisèle Bronner
Mouadh Saadaoui
Patricia Roeckel-Drevet
Mohamed Faize
Boris Fumanal
author_sort Jean-Stéphane Venisse
collection DOAJ
description The fungal kingdom, with an estimated five million species, has undergone extensive diversification over the past billion years and now occupies a wide array of ecological niches from terrestrial to aquatic ecosystems. To thrive in such diverse environments, fungi must exhibit finely tuned physiological and morphological responses orchestrated by conserved molecular pathways. Increasing evidence suggests that aquaporins (AQPs) play a key role in mediating these adaptive responses, particularly under varying abiotic and biotic stress conditions. However, despite notable advances in recent decades, the precise functional roles of AQPs within the fungal kingdom remains largely unresolved in the field of cell biology. AQPs are transmembrane proteins belonging to the major intrinsic proteins (MIPs) superfamily, which is characterized by remarkable sequence and structural diversity. Beyond their established function in facilitating water transport, MIPs mediated the bidirectional diffusion of a range of small inorganic and organic solutes, ions, and gases across cellular membranes. In fungi, MIPs are classified into three main subfamilies: orthodox (i.e., classical) AQPs, aquaglyceroporins (AQGP), and <i data-eusoft-scrollable-element="1">X</i>-intrinsic proteins (XIPs). This review provides a concise summary of the fundamental structural and functional characteristics of fungal aquaporins, including their structure, classification, and known physiological roles. While the majority of the current literature has focused on the aquaporin and aquaglyceroporin subfamilies, this review also aims to offer a comprehensive and original overview of the relatively understudied <i data-eusoft-scrollable-element="1">X</i>-intrinsic protein subfamily, highlighting its potential implication in fungal biology.
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spelling doaj-art-77fd0fa6b51640b1acd1b418f4eb6f7b2025-08-20T02:45:56ZengMDPI AGJournal of Fungi2309-608X2025-07-0111754310.3390/jof11070543Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP SubfamilyJean-Stéphane Venisse0Gisèle Bronner1Mouadh Saadaoui2Patricia Roeckel-Drevet3Mohamed Faize4Boris Fumanal5University Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, FranceCNRS, Laboratoire Microorganismes: Génome et Environnement, University Clermont Auvergne, 63000 Clermont-Ferrand, FranceUniversity Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, FranceUniversity Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, FranceLaboratory of Plant Biotechnology, Ecology and Ecosystem Valorization CNRST-URL10, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, MoroccoUniversity Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, FranceThe fungal kingdom, with an estimated five million species, has undergone extensive diversification over the past billion years and now occupies a wide array of ecological niches from terrestrial to aquatic ecosystems. To thrive in such diverse environments, fungi must exhibit finely tuned physiological and morphological responses orchestrated by conserved molecular pathways. Increasing evidence suggests that aquaporins (AQPs) play a key role in mediating these adaptive responses, particularly under varying abiotic and biotic stress conditions. However, despite notable advances in recent decades, the precise functional roles of AQPs within the fungal kingdom remains largely unresolved in the field of cell biology. AQPs are transmembrane proteins belonging to the major intrinsic proteins (MIPs) superfamily, which is characterized by remarkable sequence and structural diversity. Beyond their established function in facilitating water transport, MIPs mediated the bidirectional diffusion of a range of small inorganic and organic solutes, ions, and gases across cellular membranes. In fungi, MIPs are classified into three main subfamilies: orthodox (i.e., classical) AQPs, aquaglyceroporins (AQGP), and <i data-eusoft-scrollable-element="1">X</i>-intrinsic proteins (XIPs). This review provides a concise summary of the fundamental structural and functional characteristics of fungal aquaporins, including their structure, classification, and known physiological roles. While the majority of the current literature has focused on the aquaporin and aquaglyceroporin subfamilies, this review also aims to offer a comprehensive and original overview of the relatively understudied <i data-eusoft-scrollable-element="1">X</i>-intrinsic protein subfamily, highlighting its potential implication in fungal biology.https://www.mdpi.com/2309-608X/11/7/543aquaporinaquaglyceroporin<i>X</i>-intrinsic protein (XIP)evolutiondeep divergencephylogeny
spellingShingle Jean-Stéphane Venisse
Gisèle Bronner
Mouadh Saadaoui
Patricia Roeckel-Drevet
Mohamed Faize
Boris Fumanal
Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
Journal of Fungi
aquaporin
aquaglyceroporin
<i>X</i>-intrinsic protein (XIP)
evolution
deep divergence
phylogeny
title Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
title_full Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
title_fullStr Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
title_full_unstemmed Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
title_short Major Intrinsic Proteins in Fungi: A Special Emphasis on the XIP Subfamily
title_sort major intrinsic proteins in fungi a special emphasis on the xip subfamily
topic aquaporin
aquaglyceroporin
<i>X</i>-intrinsic protein (XIP)
evolution
deep divergence
phylogeny
url https://www.mdpi.com/2309-608X/11/7/543
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AT patriciaroeckeldrevet majorintrinsicproteinsinfungiaspecialemphasisonthexipsubfamily
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