Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients
Abstract Long noncoding RNAs (lncRNAs) play an important role in gene regulation, but their impact on the pathogenesis of colorectal cancer and the biological function of cancer cells is unclear. In this study, we used next‐generation sequencing to study the differences in the expression profiles of...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-09-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.1696 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849766672054878208 |
|---|---|
| author | Meng Li Lian‐mei Zhao Suo‐lin Li Jing Li Bo Gao Fei‐fei Wang Sheng‐pu Wang Xu‐hua Hu Jian Cao Gui‐ying Wang |
| author_facet | Meng Li Lian‐mei Zhao Suo‐lin Li Jing Li Bo Gao Fei‐fei Wang Sheng‐pu Wang Xu‐hua Hu Jian Cao Gui‐ying Wang |
| author_sort | Meng Li |
| collection | DOAJ |
| description | Abstract Long noncoding RNAs (lncRNAs) play an important role in gene regulation, but their impact on the pathogenesis of colorectal cancer and the biological function of cancer cells is unclear. In this study, we used next‐generation sequencing to study the differences in the expression profiles of lncRNAs and mRNAs in colorectal cancer tissues. We analyzed the differentially expressed genes by Gene Ontology/Kyoto Encyclopedia of Genes and Genomes (GO/KEGG) enrichment and predicted new lncRNA functions. Our results revealed that compared with lncRNAs and mRNAs in nontumor colorectal tissues, 1019 lncRNAs (512 upregulated, 507 downregulated) and 3221 mRNAs (1606 upregulated, 1615 downregulated) were differentially expressed in tumor colorectal tissues (fold change >2 and P < 0.05). We validated some of these genes by qPCR. Furthermore, we identified some new lncRNAs differently expressed in colorectal cancer samples from patients in northern China. We confirmed the function of lncRNA‐FIRRE‐201 and SLCO4A1‐AS1‐202 in colorectal cancer cells to provide an experimental basis for studies on their roles in the occurrence and development of colorectal cancer and in the regulation of networks. |
| format | Article |
| id | doaj-art-77f9762f9a2d492d8dadc813a2852879 |
| institution | DOAJ |
| issn | 2045-7634 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-77f9762f9a2d492d8dadc813a28528792025-08-20T03:04:30ZengWileyCancer Medicine2045-76342018-09-01794650466410.1002/cam4.1696Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patientsMeng Li0Lian‐mei Zhao1Suo‐lin Li2Jing Li3Bo Gao4Fei‐fei Wang5Sheng‐pu Wang6Xu‐hua Hu7Jian Cao8Gui‐ying Wang9Pediatric Surgery The Second Hospital of Hebei Medical University Shijiazhuang Hebei ChinaResearch Center Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaPediatric Surgery The Second Hospital of Hebei Medical University Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaThe Second General Surgery Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital Shijiazhuang Hebei ChinaAbstract Long noncoding RNAs (lncRNAs) play an important role in gene regulation, but their impact on the pathogenesis of colorectal cancer and the biological function of cancer cells is unclear. In this study, we used next‐generation sequencing to study the differences in the expression profiles of lncRNAs and mRNAs in colorectal cancer tissues. We analyzed the differentially expressed genes by Gene Ontology/Kyoto Encyclopedia of Genes and Genomes (GO/KEGG) enrichment and predicted new lncRNA functions. Our results revealed that compared with lncRNAs and mRNAs in nontumor colorectal tissues, 1019 lncRNAs (512 upregulated, 507 downregulated) and 3221 mRNAs (1606 upregulated, 1615 downregulated) were differentially expressed in tumor colorectal tissues (fold change >2 and P < 0.05). We validated some of these genes by qPCR. Furthermore, we identified some new lncRNAs differently expressed in colorectal cancer samples from patients in northern China. We confirmed the function of lncRNA‐FIRRE‐201 and SLCO4A1‐AS1‐202 in colorectal cancer cells to provide an experimental basis for studies on their roles in the occurrence and development of colorectal cancer and in the regulation of networks.https://doi.org/10.1002/cam4.1696colorectal cancerenrichment analysislncRNAsmRNAnext‐generation sequencing |
| spellingShingle | Meng Li Lian‐mei Zhao Suo‐lin Li Jing Li Bo Gao Fei‐fei Wang Sheng‐pu Wang Xu‐hua Hu Jian Cao Gui‐ying Wang Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients Cancer Medicine colorectal cancer enrichment analysis lncRNAs mRNA next‐generation sequencing |
| title | Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients |
| title_full | Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients |
| title_fullStr | Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients |
| title_full_unstemmed | Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients |
| title_short | Differentially expressed lncRNAs and mRNAs identified by NGS analysis in colorectal cancer patients |
| title_sort | differentially expressed lncrnas and mrnas identified by ngs analysis in colorectal cancer patients |
| topic | colorectal cancer enrichment analysis lncRNAs mRNA next‐generation sequencing |
| url | https://doi.org/10.1002/cam4.1696 |
| work_keys_str_mv | AT mengli differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT lianmeizhao differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT suolinli differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT jingli differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT bogao differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT feifeiwang differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT shengpuwang differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT xuhuahu differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT jiancao differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients AT guiyingwang differentiallyexpressedlncrnasandmrnasidentifiedbyngsanalysisincolorectalcancerpatients |