Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages
Black glutinous rice is a local pigmented rice that attracts the interest of many people due to its high nutritional value. While cultivating, black glutinous rice may experience abiotic stresses, such as drought and salinity threat. Drought and salinity may lead to oxidative stress, which leads to...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-03-01
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| Series: | Open Agriculture |
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| Online Access: | https://doi.org/10.1515/opag-2025-0417 |
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| author | Rudiyanto Yosefine Fetik Alfiana Okta Hadina Putri Anandita Linggar Rizkiantoro Ridho Siswoyo Tri Agus |
| author_facet | Rudiyanto Yosefine Fetik Alfiana Okta Hadina Putri Anandita Linggar Rizkiantoro Ridho Siswoyo Tri Agus |
| author_sort | Rudiyanto Yosefine Fetik |
| collection | DOAJ |
| description | Black glutinous rice is a local pigmented rice that attracts the interest of many people due to its high nutritional value. While cultivating, black glutinous rice may experience abiotic stresses, such as drought and salinity threat. Drought and salinity may lead to oxidative stress, which leads to the overproduction of reactive oxygen species (ROS). Moreover, the enzymatic defense mechanism of black glutinous rice against ROS, which depends on the stress type and the plant’s growth stages, remains unclear. This study was performed to determine the defense response of black glutinous rice to drought (10% PEG) and salinity (80 mM NaCl) stresses at different growth stages (vegetative [V], reproductive [R], and vegetative+reproductive [V+R]) and then continued to recover at every growth stage. This study showed enhanced accumulation of ROS under drought and salinity stresses, with the reproductive stage presenting the highest accumulation of hydrogen peroxide and malondialdehyde. In contrast, the recovery phase decreased the ROS accumulation. The antioxidant enzyme activities (catalase [CAT], ascorbate peroxidase [APX], and peroxidase [POD]) showed different responses between the biochemical and transcript levels of antioxidant genes (OsCATA, OsAPX, and OsPOD) during stress and in the recovery phase. These results indicate the foundation for elucidating the defense mechanism response of black glutinous rice to different growth stages and stresses, such as drought and salinity. |
| format | Article |
| id | doaj-art-77f50da273cb4be2aa12eb6da3102640 |
| institution | DOAJ |
| issn | 2391-9531 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Agriculture |
| spelling | doaj-art-77f50da273cb4be2aa12eb6da31026402025-08-20T02:58:40ZengDe GruyterOpen Agriculture2391-95312025-03-011015718210.1515/opag-2025-0417Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stagesRudiyanto Yosefine Fetik0Alfiana Okta Hadina1Putri Anandita Linggar2Rizkiantoro Ridho3Siswoyo Tri Agus4Graduate School of Biotechnology, University of Jember, Jl. Kalimantan No. 37, Jember, 68121, East Java, IndonesiaThe Center of Excellence on Crop Industrial Biotechnology (PUI-PT BioTIn), University of Jember, Jember, 68121, IndonesiaThe Center of Excellence on Crop Industrial Biotechnology (PUI-PT BioTIn), University of Jember, Jember, 68121, IndonesiaThe Center of Excellence on Crop Industrial Biotechnology (PUI-PT BioTIn), University of Jember, Jember, 68121, IndonesiaGraduate School of Biotechnology, University of Jember, Jl. Kalimantan No. 37, Jember, 68121, East Java, IndonesiaBlack glutinous rice is a local pigmented rice that attracts the interest of many people due to its high nutritional value. While cultivating, black glutinous rice may experience abiotic stresses, such as drought and salinity threat. Drought and salinity may lead to oxidative stress, which leads to the overproduction of reactive oxygen species (ROS). Moreover, the enzymatic defense mechanism of black glutinous rice against ROS, which depends on the stress type and the plant’s growth stages, remains unclear. This study was performed to determine the defense response of black glutinous rice to drought (10% PEG) and salinity (80 mM NaCl) stresses at different growth stages (vegetative [V], reproductive [R], and vegetative+reproductive [V+R]) and then continued to recover at every growth stage. This study showed enhanced accumulation of ROS under drought and salinity stresses, with the reproductive stage presenting the highest accumulation of hydrogen peroxide and malondialdehyde. In contrast, the recovery phase decreased the ROS accumulation. The antioxidant enzyme activities (catalase [CAT], ascorbate peroxidase [APX], and peroxidase [POD]) showed different responses between the biochemical and transcript levels of antioxidant genes (OsCATA, OsAPX, and OsPOD) during stress and in the recovery phase. These results indicate the foundation for elucidating the defense mechanism response of black glutinous rice to different growth stages and stresses, such as drought and salinity.https://doi.org/10.1515/opag-2025-0417riceoxidativeros antioxidantgene |
| spellingShingle | Rudiyanto Yosefine Fetik Alfiana Okta Hadina Putri Anandita Linggar Rizkiantoro Ridho Siswoyo Tri Agus Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages Open Agriculture rice oxidative ros antioxidant gene |
| title | Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| title_full | Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| title_fullStr | Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| title_full_unstemmed | Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| title_short | Antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| title_sort | antioxidant responses of black glutinous rice to drought and salinity stresses at different growth stages |
| topic | rice oxidative ros antioxidant gene |
| url | https://doi.org/10.1515/opag-2025-0417 |
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