Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector

A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone. The B. balsamifera extraction was separated by a Kromasil C18 column (250 × 4.6 mm, 5 μm) wi...

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Main Authors: Daopeng Tan, Zhou Yang, Qianru Zhang, Hua Ling, Yimei Du, Yanliu Lu, Tianpei Xie, Xumei Zhou, Lin Qin, Yuqi He
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2020/9731327
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author Daopeng Tan
Zhou Yang
Qianru Zhang
Hua Ling
Yimei Du
Yanliu Lu
Tianpei Xie
Xumei Zhou
Lin Qin
Yuqi He
author_facet Daopeng Tan
Zhou Yang
Qianru Zhang
Hua Ling
Yimei Du
Yanliu Lu
Tianpei Xie
Xumei Zhou
Lin Qin
Yuqi He
author_sort Daopeng Tan
collection DOAJ
description A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone. The B. balsamifera extraction was separated by a Kromasil C18 column (250 × 4.6 mm, 5 μm) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid. A photodiode array detector (PDA) was used to record the signals of investigated constituents. The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination. Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method. The 18 compounds were accurately identified by comparing with the reference compounds. The purity of each peak was confirmed by the base peak in the mass spectrum. The contents of 18 compounds in Blumea samples from four different regions were successfully determined. The results also showed that 3,3′,5,7-tetrahydroxy-4′-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of B. balsamifera and Chinese patent medications containing B. balsamifera herb.
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issn 1687-8760
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publishDate 2020-01-01
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series International Journal of Analytical Chemistry
spelling doaj-art-77f1967bc5aa441293ff4ff3af3f619b2025-02-03T01:05:29ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/97313279731327Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array DetectorDaopeng Tan0Zhou Yang1Qianru Zhang2Hua Ling3Yimei Du4Yanliu Lu5Tianpei Xie6Xumei Zhou7Lin Qin8Yuqi He9Key Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaShanghai Nature-Standard Technical Service Co., Ltd., Shanghai 201203, ChinaKey Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaSchool of Pharmacy, Georgia Campus—Philadelphia College of Osteopathic Medicine, 625 Old Peachtree Rd NW, Suwanee, GA 30024, USAKey Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaShanghai Nature-Standard Technical Service Co., Ltd., Shanghai 201203, ChinaShanghai Nature-Standard Technical Service Co., Ltd., Shanghai 201203, ChinaKey Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaKey Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaKey Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, ChinaA high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone. The B. balsamifera extraction was separated by a Kromasil C18 column (250 × 4.6 mm, 5 μm) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid. A photodiode array detector (PDA) was used to record the signals of investigated constituents. The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination. Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method. The 18 compounds were accurately identified by comparing with the reference compounds. The purity of each peak was confirmed by the base peak in the mass spectrum. The contents of 18 compounds in Blumea samples from four different regions were successfully determined. The results also showed that 3,3′,5,7-tetrahydroxy-4′-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of B. balsamifera and Chinese patent medications containing B. balsamifera herb.http://dx.doi.org/10.1155/2020/9731327
spellingShingle Daopeng Tan
Zhou Yang
Qianru Zhang
Hua Ling
Yimei Du
Yanliu Lu
Tianpei Xie
Xumei Zhou
Lin Qin
Yuqi He
Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
International Journal of Analytical Chemistry
title Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
title_full Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
title_fullStr Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
title_full_unstemmed Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
title_short Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
title_sort simultaneous quantitative determination of polyphenolic compounds in blumea balsamifera ai na xiang sembung by high performance liquid chromatography with photodiode array detector
url http://dx.doi.org/10.1155/2020/9731327
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