Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes

Pediatric acute lymphoblastic leukemia (ALL) is among the most prevalent hematological malignancies in children. Despite an overall cure rate approaching 90%, a subset of patients still experiences relapse, even with advanced therapeutic interventions. Research into the molecular characteristics an...

Full description

Saved in:
Bibliographic Details
Main Authors: Bin Zhang, Chaoran Shi, Xiuxiu Wang, Jiajia Mi, Runan Wang, Shuang Li, Jiawei Yang, Qiuying He, Yujiao Wang, Zuofei Chi, Liangchun Hao
Format: Article
Language:English
Published: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2025-05-01
Series:Biomolecules & Biomedicine
Subjects:
Online Access:https://www.bjbms.org/ojs/index.php/bjbms/article/view/12254
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850111277239631872
author Bin Zhang
Chaoran Shi
Xiuxiu Wang
Jiajia Mi
Runan Wang
Shuang Li
Jiawei Yang
Qiuying He
Yujiao Wang
Zuofei Chi
Liangchun Hao
author_facet Bin Zhang
Chaoran Shi
Xiuxiu Wang
Jiajia Mi
Runan Wang
Shuang Li
Jiawei Yang
Qiuying He
Yujiao Wang
Zuofei Chi
Liangchun Hao
author_sort Bin Zhang
collection DOAJ
description Pediatric acute lymphoblastic leukemia (ALL) is among the most prevalent hematological malignancies in children. Despite an overall cure rate approaching 90%, a subset of patients still experiences relapse, even with advanced therapeutic interventions. Research into the molecular characteristics and prognostic markers of fusion gene-negative (FG-negative) pediatric ALL remains limited. To address this gap, we performed whole-exome sequencing (WES) and whole-transcriptome sequencing (RNA-seq) on 54 FG-negative ALL cases from our center. Our results indicated that neither specific mutations nor tumor mutational burden significantly influenced relapse risk. Notably, we identified a significant downregulation of CADPS in FG-negative pediatric ALL patients who relapsed. The expression levels and prognostic significance of CADPS were further validated using data from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) cohort, where lower CADPS expression was associated with reduced event-free survival (EFS) and overall survival (OS) (P < 0.001 for both). Cox regression analyses were subsequently employed to identify OS-related factors and to construct a prognostic prediction model. Notably, this model demonstrated a significant correlation with therapeutic targets. In conclusion, our findings support the potential of CADPS expression as a novel biomarker for prognostic stratification in FG-negative pediatric ALL patients.
format Article
id doaj-art-77f03a9c9cef4e6e80637bdc8516163c
institution OA Journals
issn 2831-0896
2831-090X
language English
publishDate 2025-05-01
publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
record_format Article
series Biomolecules & Biomedicine
spelling doaj-art-77f03a9c9cef4e6e80637bdc8516163c2025-08-20T02:37:39ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-05-0110.17305/bb.2025.12254Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genesBin Zhang0Chaoran Shi1Xiuxiu Wang2Jiajia Mi3Runan Wang4Shuang Li5Jiawei Yang6Qiuying He7Yujiao Wang8Zuofei Chi9Liangchun Hao10Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Translational Medicine Center, Chigene (Beijing) Translational Medical Research Center Co., Beijing, ChinaDepartment of Translational Medicine Center, Chigene (Beijing) Translational Medical Research Center Co., Beijing, ChinaDepartment of Translational Medicine Center, Chigene (Beijing) Translational Medical Research Center Co., Beijing, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, ChinaDepartment of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China Pediatric acute lymphoblastic leukemia (ALL) is among the most prevalent hematological malignancies in children. Despite an overall cure rate approaching 90%, a subset of patients still experiences relapse, even with advanced therapeutic interventions. Research into the molecular characteristics and prognostic markers of fusion gene-negative (FG-negative) pediatric ALL remains limited. To address this gap, we performed whole-exome sequencing (WES) and whole-transcriptome sequencing (RNA-seq) on 54 FG-negative ALL cases from our center. Our results indicated that neither specific mutations nor tumor mutational burden significantly influenced relapse risk. Notably, we identified a significant downregulation of CADPS in FG-negative pediatric ALL patients who relapsed. The expression levels and prognostic significance of CADPS were further validated using data from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) cohort, where lower CADPS expression was associated with reduced event-free survival (EFS) and overall survival (OS) (P < 0.001 for both). Cox regression analyses were subsequently employed to identify OS-related factors and to construct a prognostic prediction model. Notably, this model demonstrated a significant correlation with therapeutic targets. In conclusion, our findings support the potential of CADPS expression as a novel biomarker for prognostic stratification in FG-negative pediatric ALL patients. https://www.bjbms.org/ojs/index.php/bjbms/article/view/12254Pediatric acute lymphoblastic leukemiaALLfusion gene negativeFG-negativenext-generation sequencingprognostic biomarker
spellingShingle Bin Zhang
Chaoran Shi
Xiuxiu Wang
Jiajia Mi
Runan Wang
Shuang Li
Jiawei Yang
Qiuying He
Yujiao Wang
Zuofei Chi
Liangchun Hao
Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
Biomolecules & Biomedicine
Pediatric acute lymphoblastic leukemia
ALL
fusion gene negative
FG-negative
next-generation sequencing
prognostic biomarker
title Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
title_full Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
title_fullStr Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
title_full_unstemmed Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
title_short Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
title_sort low expression of cadps predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes
topic Pediatric acute lymphoblastic leukemia
ALL
fusion gene negative
FG-negative
next-generation sequencing
prognostic biomarker
url https://www.bjbms.org/ojs/index.php/bjbms/article/view/12254
work_keys_str_mv AT binzhang lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT chaoranshi lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT xiuxiuwang lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT jiajiami lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT runanwang lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT shuangli lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT jiaweiyang lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT qiuyinghe lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT yujiaowang lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT zuofeichi lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes
AT liangchunhao lowexpressionofcadpspredictspoorprognosisinpediatricacutelymphoblasticleukemiawithoutfusiongenes