The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)

<b>Background:</b> Shiga toxin-producing <i>E. coli</i> (STEC) are important foodborne pathogens that cause serious public health consequences worldwide. This study conducted a systematic review and meta-analysis of the global prevalence of antibiotic resistance and STEC in c...

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Main Authors: Tsepo Ramatla, Nkhebenyane Jane, Mohapi Dineo, Tawana Mpho, Motlhaoloa Tshegofatso, Ntelekwane George Khasapane
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Antibiotics
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Online Access:https://www.mdpi.com/2079-6382/14/6/568
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author Tsepo Ramatla
Nkhebenyane Jane
Mohapi Dineo
Tawana Mpho
Motlhaoloa Tshegofatso
Ntelekwane George Khasapane
author_facet Tsepo Ramatla
Nkhebenyane Jane
Mohapi Dineo
Tawana Mpho
Motlhaoloa Tshegofatso
Ntelekwane George Khasapane
author_sort Tsepo Ramatla
collection DOAJ
description <b>Background:</b> Shiga toxin-producing <i>E. coli</i> (STEC) are important foodborne pathogens that cause serious public health consequences worldwide. This study conducted a systematic review and meta-analysis of the global prevalence of antibiotic resistance and STEC in chickens. <b>Methods:</b> The assessment of previous study records was carried out following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Heterogeneity between studies was assessed using Cochrane’s Q test and I<sup>2</sup> test statistics based on the random effects model, and comprehensive meta-analysis (CMA) software v4.0 was used to analyse the pooled prevalence estimate (PPE) of antibiotic resistance and STEC in chickens. <b>Results:</b> A total of 61 studies comprising 823 STEC from 18 countries were included in this study. The overall pooled prevalence of STEC was 8.9% (95% CI: 0.620–0.126). m-PCR assay showed the highest PPE of 21.0% (95%: 0.088–0.420). <i>stx1</i> had the higher PPE of 12.9% (95%: 0.081–0.199), while <i>stx2</i> had a PPE of 11.8% (95%: 0.077–0.176). Furthermore, the serotype O157 had the higher PPE of 80.5% (95%: 0.520–0.940). The isolates were resistant to the following antibiotics: amoxicillin and clavulanic acid, chloramphenicol, tetracycline, ciprofloxacin, gentamycin, ampicillin, neomycin, and amoxicillin. <b>Conclusions:</b> These findings may assist in the prevention and control of STEC in chickens globally. To minimise the spread of STEC and antibiotic resistance, future foodborne pathogen prevention and control programmes should prioritise increasing laboratory capacity for the early identification of antibiotic resistance.
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spelling doaj-art-77edda5f7a8740b1bc856b137085836d2025-08-20T03:26:48ZengMDPI AGAntibiotics2079-63822025-05-0114656810.3390/antibiotics14060568The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)Tsepo Ramatla0Nkhebenyane Jane1Mohapi Dineo2Tawana Mpho3Motlhaoloa Tshegofatso4Ntelekwane George Khasapane5Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South AfricaCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South AfricaCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South AfricaDepartment of Zoology and Entomology, University of the Free State, Private Bag x13, Phuthaditjhaba 9866, South AfricaCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South AfricaCentre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa<b>Background:</b> Shiga toxin-producing <i>E. coli</i> (STEC) are important foodborne pathogens that cause serious public health consequences worldwide. This study conducted a systematic review and meta-analysis of the global prevalence of antibiotic resistance and STEC in chickens. <b>Methods:</b> The assessment of previous study records was carried out following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Heterogeneity between studies was assessed using Cochrane’s Q test and I<sup>2</sup> test statistics based on the random effects model, and comprehensive meta-analysis (CMA) software v4.0 was used to analyse the pooled prevalence estimate (PPE) of antibiotic resistance and STEC in chickens. <b>Results:</b> A total of 61 studies comprising 823 STEC from 18 countries were included in this study. The overall pooled prevalence of STEC was 8.9% (95% CI: 0.620–0.126). m-PCR assay showed the highest PPE of 21.0% (95%: 0.088–0.420). <i>stx1</i> had the higher PPE of 12.9% (95%: 0.081–0.199), while <i>stx2</i> had a PPE of 11.8% (95%: 0.077–0.176). Furthermore, the serotype O157 had the higher PPE of 80.5% (95%: 0.520–0.940). The isolates were resistant to the following antibiotics: amoxicillin and clavulanic acid, chloramphenicol, tetracycline, ciprofloxacin, gentamycin, ampicillin, neomycin, and amoxicillin. <b>Conclusions:</b> These findings may assist in the prevention and control of STEC in chickens globally. To minimise the spread of STEC and antibiotic resistance, future foodborne pathogen prevention and control programmes should prioritise increasing laboratory capacity for the early identification of antibiotic resistance.https://www.mdpi.com/2079-6382/14/6/568Shiga toxin-producing <i>Escherichia coli</i> (STEC)chickenserotypesantibiotic resistance
spellingShingle Tsepo Ramatla
Nkhebenyane Jane
Mohapi Dineo
Tawana Mpho
Motlhaoloa Tshegofatso
Ntelekwane George Khasapane
The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
Antibiotics
Shiga toxin-producing <i>Escherichia coli</i> (STEC)
chicken
serotypes
antibiotic resistance
title The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
title_full The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
title_fullStr The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
title_full_unstemmed The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
title_short The Global Prevalence of Antibiotic Resistance and Shiga Toxin-Producing <i>Escherichia coli</i> in Chickens: A Systematic Review and Meta-Analysis (2011–2024)
title_sort global prevalence of antibiotic resistance and shiga toxin producing i escherichia coli i in chickens a systematic review and meta analysis 2011 2024
topic Shiga toxin-producing <i>Escherichia coli</i> (STEC)
chicken
serotypes
antibiotic resistance
url https://www.mdpi.com/2079-6382/14/6/568
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