Confocal Laser Scanning Microscopy

Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued developm...

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Main Author: Stephen W. Paddock
Format: Article
Language:English
Published: Taylor & Francis Group 1999-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/99275ov01
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author Stephen W. Paddock
author_facet Stephen W. Paddock
author_sort Stephen W. Paddock
collection DOAJ
description Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photostable fluorescent probes. Such advances have made possible novel experimental approaches for multiple label fluorescence, live cell imaging and multidimensional microscopy.
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1940-9818
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publishDate 1999-11-01
publisher Taylor & Francis Group
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series BioTechniques
spelling doaj-art-77ebf21b8d2f4703afee4585ab67ea5c2025-08-20T02:26:07ZengTaylor & Francis GroupBioTechniques0736-62051940-98181999-11-01275992100410.2144/99275ov01Confocal Laser Scanning MicroscopyStephen W. Paddock01University of Wisconsin, Madison, WI, USAMany technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photostable fluorescent probes. Such advances have made possible novel experimental approaches for multiple label fluorescence, live cell imaging and multidimensional microscopy.https://www.future-science.com/doi/10.2144/99275ov01
spellingShingle Stephen W. Paddock
Confocal Laser Scanning Microscopy
BioTechniques
title Confocal Laser Scanning Microscopy
title_full Confocal Laser Scanning Microscopy
title_fullStr Confocal Laser Scanning Microscopy
title_full_unstemmed Confocal Laser Scanning Microscopy
title_short Confocal Laser Scanning Microscopy
title_sort confocal laser scanning microscopy
url https://www.future-science.com/doi/10.2144/99275ov01
work_keys_str_mv AT stephenwpaddock confocallaserscanningmicroscopy