Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey
Abstract The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing K. xylinus with selected s...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-08-01
|
| Series: | Applied Microbiology and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s00253-025-13582-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849226504681029632 |
|---|---|
| author | Justyna Płoska Monika Garbowska Iwona Ścibisz Lidia Stasiak-Różańska |
| author_facet | Justyna Płoska Monika Garbowska Iwona Ścibisz Lidia Stasiak-Różańska |
| author_sort | Justyna Płoska |
| collection | DOAJ |
| description | Abstract The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing K. xylinus with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in K. xylinus monoculture. The K. xylinus + Lb. acidophilus co-culture yielded 2.19 g·L−1 of BC, which was 125% more than the K. xylinus monoculture. Additionally, K. xylinus in co-culture with Lb. acidophilus increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with Lb. delbrueckii showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with Lb. acidophilus and stress at break BC from co-culture with Lb. helveticus were 72% and 54% higher, respectively, than BC obtained from monoculture K. xylinus. In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product. Key points • The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture. • High lactic acid content and low pH of acid whey enhance cellulose biosynthesis. • Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose. |
| format | Article |
| id | doaj-art-77e83c2e3c49496eb3da4e5b93137a67 |
| institution | Kabale University |
| issn | 1432-0614 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Springer |
| record_format | Article |
| series | Applied Microbiology and Biotechnology |
| spelling | doaj-art-77e83c2e3c49496eb3da4e5b93137a672025-08-24T11:13:35ZengSpringerApplied Microbiology and Biotechnology1432-06142025-08-01109111910.1007/s00253-025-13582-3Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in wheyJustyna Płoska0Monika Garbowska1Iwona Ścibisz2Lidia Stasiak-Różańska3Department of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences - SGGWDepartment of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences - SGGWDepartment of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences - SGGWDepartment of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences - SGGWAbstract The use of acid whey as a medium is an innovative approach to bacterial cellulose (BC) biosynthesis in co-cultures of acetic acid bacteria with lactic acid bacteria. The aim of this study was to evaluate the possibility of obtaining BC in acid whey by co-culturing K. xylinus with selected strains of lactic acid bacteria and comparing the properties of this biopolymer with BC obtained in K. xylinus monoculture. The K. xylinus + Lb. acidophilus co-culture yielded 2.19 g·L−1 of BC, which was 125% more than the K. xylinus monoculture. Additionally, K. xylinus in co-culture with Lb. acidophilus increased the degradation temperature of BC to 361 °C compared to 303 °C for BC obtained in monoculture. The BC obtained in the co-cultures showed better mechanical properties. BC obtained in co-culture with Lb. delbrueckii showed more than twice the Young’s modulus than BC from monoculture. Moreover, strain at break BC from co-culture with Lb. acidophilus and stress at break BC from co-culture with Lb. helveticus were 72% and 54% higher, respectively, than BC obtained from monoculture K. xylinus. In this study, it was shown that conducting acetic-lactic co-cultures increased the efficiency of BC biosynthesis and improved its properties. Moreover, this study has shown that acid whey is a sufficient and complete substrate for obtaining BC. Results presented in this paper indicate new possibilities for the management of this side product. Key points • The K. xylinus + Lb. acidophilus co-culture produced 125% more cellulose than the monoculture. • High lactic acid content and low pH of acid whey enhance cellulose biosynthesis. • Acetic acid-lactic acid co-cultures improved the mechanical properties of cellulose.https://doi.org/10.1007/s00253-025-13582-3Bacterial celluloseKomagataeibacter xylinusLactic acid bacteriaLactobacillusLactic acidAcid whey |
| spellingShingle | Justyna Płoska Monika Garbowska Iwona Ścibisz Lidia Stasiak-Różańska Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey Applied Microbiology and Biotechnology Bacterial cellulose Komagataeibacter xylinus Lactic acid bacteria Lactobacillus Lactic acid Acid whey |
| title | Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey |
| title_full | Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey |
| title_fullStr | Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey |
| title_full_unstemmed | Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey |
| title_short | Study on obtaining bacterial cellulose by Komagataeibacter xylinus in co-culture with lactic acid bacteria in whey |
| title_sort | study on obtaining bacterial cellulose by komagataeibacter xylinus in co culture with lactic acid bacteria in whey |
| topic | Bacterial cellulose Komagataeibacter xylinus Lactic acid bacteria Lactobacillus Lactic acid Acid whey |
| url | https://doi.org/10.1007/s00253-025-13582-3 |
| work_keys_str_mv | AT justynapłoska studyonobtainingbacterialcellulosebykomagataeibacterxylinusincoculturewithlacticacidbacteriainwhey AT monikagarbowska studyonobtainingbacterialcellulosebykomagataeibacterxylinusincoculturewithlacticacidbacteriainwhey AT iwonascibisz studyonobtainingbacterialcellulosebykomagataeibacterxylinusincoculturewithlacticacidbacteriainwhey AT lidiastasiakrozanska studyonobtainingbacterialcellulosebykomagataeibacterxylinusincoculturewithlacticacidbacteriainwhey |