In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes
Circulating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development. However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays. Alternatively, tumor-associated particles (CTPs) including exosomes can...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2016/1628057 |
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| author | Jacqueline Nolan Mustafa Sarimollaoglu Dmitry A. Nedosekin Azemat Jamshidi-Parsian Ekaterina I. Galanzha Rajshekhar A. Kore Robert J. Griffin Vladimir P. Zharov |
| author_facet | Jacqueline Nolan Mustafa Sarimollaoglu Dmitry A. Nedosekin Azemat Jamshidi-Parsian Ekaterina I. Galanzha Rajshekhar A. Kore Robert J. Griffin Vladimir P. Zharov |
| author_sort | Jacqueline Nolan |
| collection | DOAJ |
| description | Circulating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development. However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays. Alternatively, tumor-associated particles (CTPs) including exosomes can be a more valuable prognostic marker because they can be released from the primary tumor long before CTCs and in larger amount. However, little progress has been made in high sensitivity detection of CTPs, especially in vivo. We show here that in vivo integrated photoacoustic (PA) and fluorescence flow cytometry (PAFFC) platform can provide the detection of melanoma and breast-cancer-associated single CTPs with endogenously expressed melanin and genetically engineered proteins or exogenous dyes as PA and fluorescent contrast agents. The two-beam, time-of-light PAFFC can measure the sizes of CTCs and CTPs and identify bulk and rolling CTCs and CTC clusters, with no influence on blood flow instability. This technique revealed a higher concentration of CTPs than CTCs at an early cancer stage. Because a single tumor cell can release many CTPs and in vivo PAFFC can examine the whole blood volume, PAFFC diagnostic platform has the potential to dramatically improve (up to 105-fold) the sensitivity of cancer diagnosis. |
| format | Article |
| id | doaj-art-77e6e46bb32943bcbdd688739436ce68 |
| institution | OA Journals |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-77e6e46bb32943bcbdd688739436ce682025-08-20T02:07:09ZengWileyAnalytical Cellular Pathology2210-71772210-71852016-01-01201610.1155/2016/16280571628057In Vivo Flow Cytometry of Circulating Tumor-Associated ExosomesJacqueline Nolan0Mustafa Sarimollaoglu1Dmitry A. Nedosekin2Azemat Jamshidi-Parsian3Ekaterina I. Galanzha4Rajshekhar A. Kore5Robert J. Griffin6Vladimir P. Zharov7Department of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAArkansas Nanomedicine Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAArkansas Nanomedicine Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAArkansas Nanomedicine Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USACirculating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development. However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays. Alternatively, tumor-associated particles (CTPs) including exosomes can be a more valuable prognostic marker because they can be released from the primary tumor long before CTCs and in larger amount. However, little progress has been made in high sensitivity detection of CTPs, especially in vivo. We show here that in vivo integrated photoacoustic (PA) and fluorescence flow cytometry (PAFFC) platform can provide the detection of melanoma and breast-cancer-associated single CTPs with endogenously expressed melanin and genetically engineered proteins or exogenous dyes as PA and fluorescent contrast agents. The two-beam, time-of-light PAFFC can measure the sizes of CTCs and CTPs and identify bulk and rolling CTCs and CTC clusters, with no influence on blood flow instability. This technique revealed a higher concentration of CTPs than CTCs at an early cancer stage. Because a single tumor cell can release many CTPs and in vivo PAFFC can examine the whole blood volume, PAFFC diagnostic platform has the potential to dramatically improve (up to 105-fold) the sensitivity of cancer diagnosis.http://dx.doi.org/10.1155/2016/1628057 |
| spellingShingle | Jacqueline Nolan Mustafa Sarimollaoglu Dmitry A. Nedosekin Azemat Jamshidi-Parsian Ekaterina I. Galanzha Rajshekhar A. Kore Robert J. Griffin Vladimir P. Zharov In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes Analytical Cellular Pathology |
| title | In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes |
| title_full | In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes |
| title_fullStr | In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes |
| title_full_unstemmed | In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes |
| title_short | In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes |
| title_sort | in vivo flow cytometry of circulating tumor associated exosomes |
| url | http://dx.doi.org/10.1155/2016/1628057 |
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