<i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study
Parkinson’s disease (PD) is the second most common neurodegenerative disease, and its prevalence is expected to double in the next 30 years. Currently, no effective treatment exists for Parkinson’s disease. Thus, the research has focused on discovering new natural compounds with strong neuroprotecti...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/3/174 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850089722639024128 |
|---|---|
| author | Muazzez Derya-Andeden Pinar Altin-Celik Enver Ersoy Andeden Hamiyet Donmez-Altuntas |
| author_facet | Muazzez Derya-Andeden Pinar Altin-Celik Enver Ersoy Andeden Hamiyet Donmez-Altuntas |
| author_sort | Muazzez Derya-Andeden |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is the second most common neurodegenerative disease, and its prevalence is expected to double in the next 30 years. Currently, no effective treatment exists for Parkinson’s disease. Thus, the research has focused on discovering new natural compounds with strong neuroprotective potential. This study aimed to investigate the effects of the methanol extract of <i>Desmodesmus arthrodesmiformis</i> EM13 (DaMe) on the mitochondrial damage pathway in an in vitro model of PD. The isolate of <i>Desmodesmus arthrodesmiformis</i> EM13 was first grown under appropriate culture conditions, and then the extract (DaMe) was prepared for use in the experiments. The total lipid and protein contents, fatty acid composition, and elemental content of DaMe were subsequently determined. Human SH-SY5Y neuroblastoma cells were pretreated with nontoxic concentrations of DaMe before 6-hydroxydopamine (6-OHDA) toxicity. Pretreatment with DaMe at concentrations of 100, 250, and 500 µg/mL showed a neuroprotective effect on 6-OHDA-induced SH-SY5Y neuroblastoma cells by decreasing the reactive oxygen species (ROS) production, decreasing the total oxidant status (TOS), increasing the total antioxidant capacity (TAC), increasing the mitochondrial membrane potential (ΔΨm), decreasing the oxidative DNA damage, and regulating gene expressions related to PD and apoptosis. Given the results of our study, we suggest that DaMe can be used as a natural source for producing drugs and dietary supplements intended to treat PD. |
| format | Article |
| id | doaj-art-77e596d239304ffa89e3984c10c2b032 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-77e596d239304ffa89e3984c10c2b0322025-08-20T02:42:42ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-03-0147317410.3390/cimb47030174<i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro StudyMuazzez Derya-Andeden0Pinar Altin-Celik1Enver Ersoy Andeden2Hamiyet Donmez-Altuntas3Department of Medical Biology, Faculty of Medicine, Erciyes University, 38030 Kayseri, TürkiyeDepartment of Medical Biology, Faculty of Medicine, Erciyes University, 38030 Kayseri, TürkiyeDepartment of Molecular Biology and Genetics, Faculty of Art and Science, Hacı Bektaş Veli University, 50300 Nevşehir, TürkiyeDepartment of Medical Biology, Faculty of Medicine, Erciyes University, 38030 Kayseri, TürkiyeParkinson’s disease (PD) is the second most common neurodegenerative disease, and its prevalence is expected to double in the next 30 years. Currently, no effective treatment exists for Parkinson’s disease. Thus, the research has focused on discovering new natural compounds with strong neuroprotective potential. This study aimed to investigate the effects of the methanol extract of <i>Desmodesmus arthrodesmiformis</i> EM13 (DaMe) on the mitochondrial damage pathway in an in vitro model of PD. The isolate of <i>Desmodesmus arthrodesmiformis</i> EM13 was first grown under appropriate culture conditions, and then the extract (DaMe) was prepared for use in the experiments. The total lipid and protein contents, fatty acid composition, and elemental content of DaMe were subsequently determined. Human SH-SY5Y neuroblastoma cells were pretreated with nontoxic concentrations of DaMe before 6-hydroxydopamine (6-OHDA) toxicity. Pretreatment with DaMe at concentrations of 100, 250, and 500 µg/mL showed a neuroprotective effect on 6-OHDA-induced SH-SY5Y neuroblastoma cells by decreasing the reactive oxygen species (ROS) production, decreasing the total oxidant status (TOS), increasing the total antioxidant capacity (TAC), increasing the mitochondrial membrane potential (ΔΨm), decreasing the oxidative DNA damage, and regulating gene expressions related to PD and apoptosis. Given the results of our study, we suggest that DaMe can be used as a natural source for producing drugs and dietary supplements intended to treat PD.https://www.mdpi.com/1467-3045/47/3/174Parkinson’s diseasemicroalgaeSH-SY5Y6-OHDAmitochondrial damageoxidative stress |
| spellingShingle | Muazzez Derya-Andeden Pinar Altin-Celik Enver Ersoy Andeden Hamiyet Donmez-Altuntas <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study Current Issues in Molecular Biology Parkinson’s disease microalgae SH-SY5Y 6-OHDA mitochondrial damage oxidative stress |
| title | <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study |
| title_full | <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study |
| title_fullStr | <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study |
| title_full_unstemmed | <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study |
| title_short | <i>Desmodesmus</i> Extract as a Mitochondrion-Targeted Neuroprotective Agent in Parkinson’s Disease: An In Vitro Study |
| title_sort | i desmodesmus i extract as a mitochondrion targeted neuroprotective agent in parkinson s disease an in vitro study |
| topic | Parkinson’s disease microalgae SH-SY5Y 6-OHDA mitochondrial damage oxidative stress |
| url | https://www.mdpi.com/1467-3045/47/3/174 |
| work_keys_str_mv | AT muazzezderyaandeden idesmodesmusiextractasamitochondriontargetedneuroprotectiveagentinparkinsonsdiseaseaninvitrostudy AT pinaraltincelik idesmodesmusiextractasamitochondriontargetedneuroprotectiveagentinparkinsonsdiseaseaninvitrostudy AT enverersoyandeden idesmodesmusiextractasamitochondriontargetedneuroprotectiveagentinparkinsonsdiseaseaninvitrostudy AT hamiyetdonmezaltuntas idesmodesmusiextractasamitochondriontargetedneuroprotectiveagentinparkinsonsdiseaseaninvitrostudy |