Cutting-edge assessment techniques for B cell immune memory: an overview
The adaptive humoral immune response depends on B cells. After antigens are processed and presented to naive B cells, these B cells differentiate into either surface immunoglobulin-expressing memory B cells (MBCs) or antibody-secreting plasma cells (PC) and long-lived plasma cells (LLPC). In paralle...
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Taylor & Francis Group
2024-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2345119 |
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| author | Martina Bozhkova Petya Gardzheva Vanya Rangelova Hristo Taskov Marianna Murdjeva |
| author_facet | Martina Bozhkova Petya Gardzheva Vanya Rangelova Hristo Taskov Marianna Murdjeva |
| author_sort | Martina Bozhkova |
| collection | DOAJ |
| description | The adaptive humoral immune response depends on B cells. After antigens are processed and presented to naive B cells, these B cells differentiate into either surface immunoglobulin-expressing memory B cells (MBCs) or antibody-secreting plasma cells (PC) and long-lived plasma cells (LLPC). In parallel with the detection of antibodies, the detection of MBCs and LLPC is important to assess the strength and duration of the humoral immune response. The number of antigen-specific cells can be determined by the secretion of antibodies using highly sensitive techniques such as ELISpot or FluoroSpot. Flow cytometry is usually used to determine the antigen-specific MBC, PC and LLPC by binding of antigens to their surface immunoglobulins. In this technique, the antigen is either directly labelled with a fluorochrome or non-covalently linked to labelled streptavidin in a tetramer. The advantage of flow cytometry is that detailed phenotypic analysis of B cell subpopulations can be performed. Single-cell RNA Sequencing provides in-depth information about B-cell receptor diversity, functional states, transcriptomic changes associated with rare cell populations or dynamic cellular processes within the immune system. Additionally, it can lead to the discovery of novel B cell subsets previously unknown. The advantages, disadvantages and potential applications of each technique are thoroughly outlined. This overview of current knowledge on B-cell immune memory highlights some implications for advancing vaccination strategies, autoimmune disease management and personalized medicine. |
| format | Article |
| id | doaj-art-77e4be2dbfca4ab28b5177d60ff07d44 |
| institution | OA Journals |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-77e4be2dbfca4ab28b5177d60ff07d442025-08-20T02:22:10ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2345119Cutting-edge assessment techniques for B cell immune memory: an overviewMartina Bozhkova0Petya Gardzheva1Vanya Rangelova2Hristo Taskov3Marianna Murdjeva4Department of Medical Microbiology and Immunology “Prof. Elisey Yanev, MD”, Medical University–Plovdiv, Plovdiv, BulgariaDepartment of Medical Microbiology and Immunology “Prof. Elisey Yanev, MD”, Medical University–Plovdiv, Plovdiv, BulgariaDepartment of Epidemiology and Disaster Medicine, Faculty of Public Health, Medical University–Plovdiv, Plovdiv, BulgariaResearch Institute, Medical University–Plovdiv, Plovdiv, BulgariaDepartment of Medical Microbiology and Immunology “Prof. Elisey Yanev, MD”, Medical University–Plovdiv, Plovdiv, BulgariaThe adaptive humoral immune response depends on B cells. After antigens are processed and presented to naive B cells, these B cells differentiate into either surface immunoglobulin-expressing memory B cells (MBCs) or antibody-secreting plasma cells (PC) and long-lived plasma cells (LLPC). In parallel with the detection of antibodies, the detection of MBCs and LLPC is important to assess the strength and duration of the humoral immune response. The number of antigen-specific cells can be determined by the secretion of antibodies using highly sensitive techniques such as ELISpot or FluoroSpot. Flow cytometry is usually used to determine the antigen-specific MBC, PC and LLPC by binding of antigens to their surface immunoglobulins. In this technique, the antigen is either directly labelled with a fluorochrome or non-covalently linked to labelled streptavidin in a tetramer. The advantage of flow cytometry is that detailed phenotypic analysis of B cell subpopulations can be performed. Single-cell RNA Sequencing provides in-depth information about B-cell receptor diversity, functional states, transcriptomic changes associated with rare cell populations or dynamic cellular processes within the immune system. Additionally, it can lead to the discovery of novel B cell subsets previously unknown. The advantages, disadvantages and potential applications of each technique are thoroughly outlined. This overview of current knowledge on B-cell immune memory highlights some implications for advancing vaccination strategies, autoimmune disease management and personalized medicine.https://www.tandfonline.com/doi/10.1080/13102818.2024.2345119B cellsB lymphocytes subpopulationsB cellular immune responseMethods for B cells assessment |
| spellingShingle | Martina Bozhkova Petya Gardzheva Vanya Rangelova Hristo Taskov Marianna Murdjeva Cutting-edge assessment techniques for B cell immune memory: an overview Biotechnology & Biotechnological Equipment B cells B lymphocytes subpopulations B cellular immune response Methods for B cells assessment |
| title | Cutting-edge assessment techniques for B cell immune memory: an overview |
| title_full | Cutting-edge assessment techniques for B cell immune memory: an overview |
| title_fullStr | Cutting-edge assessment techniques for B cell immune memory: an overview |
| title_full_unstemmed | Cutting-edge assessment techniques for B cell immune memory: an overview |
| title_short | Cutting-edge assessment techniques for B cell immune memory: an overview |
| title_sort | cutting edge assessment techniques for b cell immune memory an overview |
| topic | B cells B lymphocytes subpopulations B cellular immune response Methods for B cells assessment |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2345119 |
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