EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection
It has been reported that IFN-λs inhibit HCV replication in vitro. But the mechanisms of how IL-28A conducts antiviral activity and the functions of IL-28A-induced ISGs (IFN-stimulated genes) are not fully understood. In this study, we found that IL-28A has the antiviral effect on HCV life cycle inc...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/716315 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850220157981425664 |
|---|---|
| author | Xianghe Meng Darong Yang Rong Yu Haizhen Zhu |
| author_facet | Xianghe Meng Darong Yang Rong Yu Haizhen Zhu |
| author_sort | Xianghe Meng |
| collection | DOAJ |
| description | It has been reported that IFN-λs inhibit HCV replication in vitro. But the mechanisms of how IL-28A conducts antiviral activity and the functions of IL-28A-induced ISGs (IFN-stimulated genes) are not fully understood. In this study, we found that IL-28A has the antiviral effect on HCV life cycle including viral replication, assembly, and release. IL-28A and IFN-α synergistically inhibit virus replication. EPSTI1 (epithelial-stromal interaction 1), one of IL-28A-induced ISGs, plays a vital role in IL-28A-mediated antiviral activity. Furthermore, forced expression of EPSTI1 effectively inhibits HCV replication in the absence of interferon treatment, and knockdown of EPSTI1 contributes to viral enhancement. EPSTI1 can activate PKR promoter and induce several PKR-dependent genes, including IFN-β, IFIT1, OAS1, and RNase L, which is responsible for EPSTI1-mediated antiviral activity. |
| format | Article |
| id | doaj-art-77da4d7cca804db28979e960f85dfbde |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-77da4d7cca804db28979e960f85dfbde2025-08-20T02:07:09ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/716315716315EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV InfectionXianghe Meng0Darong Yang1Rong Yu2Haizhen Zhu3Department of Molecular Medicine of College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, ChinaDepartment of Molecular Medicine of College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, ChinaDepartment of Molecular Medicine of College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, ChinaDepartment of Molecular Medicine of College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, ChinaIt has been reported that IFN-λs inhibit HCV replication in vitro. But the mechanisms of how IL-28A conducts antiviral activity and the functions of IL-28A-induced ISGs (IFN-stimulated genes) are not fully understood. In this study, we found that IL-28A has the antiviral effect on HCV life cycle including viral replication, assembly, and release. IL-28A and IFN-α synergistically inhibit virus replication. EPSTI1 (epithelial-stromal interaction 1), one of IL-28A-induced ISGs, plays a vital role in IL-28A-mediated antiviral activity. Furthermore, forced expression of EPSTI1 effectively inhibits HCV replication in the absence of interferon treatment, and knockdown of EPSTI1 contributes to viral enhancement. EPSTI1 can activate PKR promoter and induce several PKR-dependent genes, including IFN-β, IFIT1, OAS1, and RNase L, which is responsible for EPSTI1-mediated antiviral activity.http://dx.doi.org/10.1155/2015/716315 |
| spellingShingle | Xianghe Meng Darong Yang Rong Yu Haizhen Zhu EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection Mediators of Inflammation |
| title | EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection |
| title_full | EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection |
| title_fullStr | EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection |
| title_full_unstemmed | EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection |
| title_short | EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection |
| title_sort | epsti1 is involved in il 28a mediated inhibition of hcv infection |
| url | http://dx.doi.org/10.1155/2015/716315 |
| work_keys_str_mv | AT xianghemeng epsti1isinvolvedinil28amediatedinhibitionofhcvinfection AT darongyang epsti1isinvolvedinil28amediatedinhibitionofhcvinfection AT rongyu epsti1isinvolvedinil28amediatedinhibitionofhcvinfection AT haizhenzhu epsti1isinvolvedinil28amediatedinhibitionofhcvinfection |