HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway
Summary: In Arabidopsis, late endosome-localized ABNORMAL SHOOT 3 (ABS3) promotes senescence through a direct interaction with AUTOPHAGY8 (ATG8). However, the molecular mechanisms underlying the regulation of the ABS3-mediated senescence pathway are not well understood. Here, we report that HOOKLESS...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725002785 |
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| author | Pei Lei Zhaoliang Zhang Xue An Lixuan Feng Xiao Shen Hui Xue Liangchen Xu Jingxia Shao Fei Yu Xiayan Liu |
| author_facet | Pei Lei Zhaoliang Zhang Xue An Lixuan Feng Xiao Shen Hui Xue Liangchen Xu Jingxia Shao Fei Yu Xiayan Liu |
| author_sort | Pei Lei |
| collection | DOAJ |
| description | Summary: In Arabidopsis, late endosome-localized ABNORMAL SHOOT 3 (ABS3) promotes senescence through a direct interaction with AUTOPHAGY8 (ATG8). However, the molecular mechanisms underlying the regulation of the ABS3-mediated senescence pathway are not well understood. Here, we report that HOOKLESS 1 (HLS1) acts as a negative regulator of plant senescence and the ABS3-mediated senescence pathway. We identify the localizations of HLS1 at the plasma membrane and endosomes in addition to the nucleus. Mechanistically, non-nucleus-localized HLS1 directly interacts with ATG8, attenuates the ABS3-ATG8 interaction, and inhibits the vacuolar degradation of ABS3, thereby antagonizing the senescence-promoting role of ABS3. Additionally, we show that the stability of HLS1 itself is negatively regulated during carbon deprivation-induced senescence. Finally, we find that HLS1 homologs in both Arabidopsis and wheat play a conserved role in senescence regulation. In summary, our findings show the functional link between HLS1, ABS3, and ATG8 in plant senescence regulation. |
| format | Article |
| id | doaj-art-77cea8a9f5e24e328e466e25f3408521 |
| institution | DOAJ |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-77cea8a9f5e24e328e466e25f34085212025-08-20T03:08:20ZengElsevierCell Reports2211-12472025-04-0144411550710.1016/j.celrep.2025.115507HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathwayPei Lei0Zhaoliang Zhang1Xue An2Lixuan Feng3Xiao Shen4Hui Xue5Liangchen Xu6Jingxia Shao7Fei Yu8Xiayan Liu9State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China; Institute of Future Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; Corresponding authorState Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China; Corresponding authorSummary: In Arabidopsis, late endosome-localized ABNORMAL SHOOT 3 (ABS3) promotes senescence through a direct interaction with AUTOPHAGY8 (ATG8). However, the molecular mechanisms underlying the regulation of the ABS3-mediated senescence pathway are not well understood. Here, we report that HOOKLESS 1 (HLS1) acts as a negative regulator of plant senescence and the ABS3-mediated senescence pathway. We identify the localizations of HLS1 at the plasma membrane and endosomes in addition to the nucleus. Mechanistically, non-nucleus-localized HLS1 directly interacts with ATG8, attenuates the ABS3-ATG8 interaction, and inhibits the vacuolar degradation of ABS3, thereby antagonizing the senescence-promoting role of ABS3. Additionally, we show that the stability of HLS1 itself is negatively regulated during carbon deprivation-induced senescence. Finally, we find that HLS1 homologs in both Arabidopsis and wheat play a conserved role in senescence regulation. In summary, our findings show the functional link between HLS1, ABS3, and ATG8 in plant senescence regulation.http://www.sciencedirect.com/science/article/pii/S2211124725002785CP: Plants |
| spellingShingle | Pei Lei Zhaoliang Zhang Xue An Lixuan Feng Xiao Shen Hui Xue Liangchen Xu Jingxia Shao Fei Yu Xiayan Liu HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway Cell Reports CP: Plants |
| title | HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway |
| title_full | HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway |
| title_fullStr | HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway |
| title_full_unstemmed | HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway |
| title_short | HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway |
| title_sort | hls1 interacts with atg8 to negatively regulate the abs3 mediated plant senescence pathway |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725002785 |
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