Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells
Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collection of these cells can be achieved through...
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MDPI AG
2025-02-01
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| author | Yasmin Godoi dos Reis Maria Eduarda Pralon Guerra Meline de Paula Coutinho Sarah Ingrid Pinto Santos Bruna Dias Mota Lauriene Luiza de Souza Munhoz Diogo Pascoal Rossetti Daniele dos Santos Martins |
| author_facet | Yasmin Godoi dos Reis Maria Eduarda Pralon Guerra Meline de Paula Coutinho Sarah Ingrid Pinto Santos Bruna Dias Mota Lauriene Luiza de Souza Munhoz Diogo Pascoal Rossetti Daniele dos Santos Martins |
| author_sort | Yasmin Godoi dos Reis |
| collection | DOAJ |
| description | Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collection of these cells can be achieved through feather plucking. Feather samples were opened with a scalpel and the feather pulp was washed with PBS, cut into cubes and digested in collagenase type IV. Cultivation was carried out in DMEM culture medium with 15% fetal bovine serum, 1% penicillin/streptomycin and 0.5% amphotericin, under incubation conditions of 39.5 °C and 5% CO<sub>2</sub>. Passages were carried out with 5% EDTA for 5 min. The culture was successful, with great cell proliferation, adherence to plastic and aggregation into cell colonies. This method was effective in obtaining feather follicle cells from wild birds, especially when collected up to 6 h after their death, and can serve as a base protocol for research with feather follicle cells aiming to create biobanks. |
| format | Article |
| id | doaj-art-77a64bae151f45b3b39730a2e3a0b92b |
| institution | OA Journals |
| issn | 2409-9279 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Methods and Protocols |
| spelling | doaj-art-77a64bae151f45b3b39730a2e3a0b92b2025-08-20T02:03:41ZengMDPI AGMethods and Protocols2409-92792025-02-01811710.3390/mps8010017Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem CellsYasmin Godoi dos Reis0Maria Eduarda Pralon Guerra1Meline de Paula Coutinho2Sarah Ingrid Pinto Santos3Bruna Dias Mota4Lauriene Luiza de Souza Munhoz5Diogo Pascoal Rossetti6Daniele dos Santos Martins7Laboratory of Immunohistochemistry and Experimental Physiology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilLaboratory of Immunohistochemistry and Experimental Physiology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilLaboratory of Stem Cells and Gene Therapy, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilLaboratory of Stem Cells and Gene Therapy, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilLaboratory of Immunohistochemistry and Experimental Physiology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilLaboratory of Immunohistochemistry and Experimental Physiology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilZoo das Aves, Poços de Caldas 37704-397, MG, BrazilLaboratory of Immunohistochemistry and Experimental Physiology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, SP, BrazilFollicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fibroblasts. Collection of these cells can be achieved through feather plucking. Feather samples were opened with a scalpel and the feather pulp was washed with PBS, cut into cubes and digested in collagenase type IV. Cultivation was carried out in DMEM culture medium with 15% fetal bovine serum, 1% penicillin/streptomycin and 0.5% amphotericin, under incubation conditions of 39.5 °C and 5% CO<sub>2</sub>. Passages were carried out with 5% EDTA for 5 min. The culture was successful, with great cell proliferation, adherence to plastic and aggregation into cell colonies. This method was effective in obtaining feather follicle cells from wild birds, especially when collected up to 6 h after their death, and can serve as a base protocol for research with feather follicle cells aiming to create biobanks.https://www.mdpi.com/2409-9279/8/1/17feather follicle cell culturewild birdsbiobanks |
| spellingShingle | Yasmin Godoi dos Reis Maria Eduarda Pralon Guerra Meline de Paula Coutinho Sarah Ingrid Pinto Santos Bruna Dias Mota Lauriene Luiza de Souza Munhoz Diogo Pascoal Rossetti Daniele dos Santos Martins Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells Methods and Protocols feather follicle cell culture wild birds biobanks |
| title | Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells |
| title_full | Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells |
| title_fullStr | Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells |
| title_full_unstemmed | Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells |
| title_short | Wild Birds’ Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells |
| title_sort | wild birds genetic resources bank feather follicle cell culture as a possible source of stem cells |
| topic | feather follicle cell culture wild birds biobanks |
| url | https://www.mdpi.com/2409-9279/8/1/17 |
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