Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial

The effect of single-nanosized oxygen nanobubble water (NBO2W), an oxygen-enriched beverage, on exercise performance remains unclear. This double-blind, randomized, placebo-controlled crossover trial investigated whether NBO2W intake influences lactate threshold (LT), a key indicator of aerobic exer...

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Main Authors: Yuki Muramoto, Noriyuki Ishida, Emi Minaguchi, Kazuhisa Sugai, Kyohei Daigo, Yuji Iwasawa, Genki Ichihara, Kengo Nagashima, Yasunori Sato, Kazuki Sato, Yoshinori Katsumata
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Physiology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665944125000215
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author Yuki Muramoto
Noriyuki Ishida
Emi Minaguchi
Kazuhisa Sugai
Kyohei Daigo
Yuji Iwasawa
Genki Ichihara
Kengo Nagashima
Yasunori Sato
Kazuki Sato
Yoshinori Katsumata
author_facet Yuki Muramoto
Noriyuki Ishida
Emi Minaguchi
Kazuhisa Sugai
Kyohei Daigo
Yuji Iwasawa
Genki Ichihara
Kengo Nagashima
Yasunori Sato
Kazuki Sato
Yoshinori Katsumata
author_sort Yuki Muramoto
collection DOAJ
description The effect of single-nanosized oxygen nanobubble water (NBO2W), an oxygen-enriched beverage, on exercise performance remains unclear. This double-blind, randomized, placebo-controlled crossover trial investigated whether NBO2W intake influences lactate threshold (LT), a key indicator of aerobic exercise performance. The participants (n = 20; age, 47.4 ± 5.0 years; female, 12) consumed either NBO2W or placebo water (PW) immediately followed by an incremental exercise test on an ergometer for symptom-limited exhaustion. During the test, the heart rate, respiratory gas analysis (VO2 and VCO2), tissue oxygen index (TOI), and blood lactate level were continuously monitored. The first administered beverage was randomly selected, with both participants and investigators blinded to the beverages. A washout period of minimum seven days was implemented between the two tests. The mean peak VO2 was 26.13 ± 5.05 mL/min/kg. Eighteen (90 %) participants exercised less than four times per week, indicating limited exercise habits. The average washout period was 9.7 ± 5.0 days. The work rate (WR) at LT was significantly higher after NBO2W intake than after PW intake (mean difference = 3.33 W, p = 0.03). The WR was not significantly different at peak VO2 or at the onset of blood lactate accumulation. Furthermore, NBO2W intake significantly increased the lactate levels 5 min post-exercise (mean difference = 0.88, p = 0.01) and decreased the TOI (mean difference = −3.8, p = 0.01). Short-term NBO2W consumption in three doses, including that on the day before exercise, did not affect the peak exercise capacity, but improved the WR at LT.
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publishDate 2025-01-01
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spelling doaj-art-77a44bf4acc449e6b5fed6b88e799a4b2025-08-20T03:40:37ZengElsevierCurrent Research in Physiology2665-94412025-01-01810015910.1016/j.crphys.2025.100159Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trialYuki Muramoto0Noriyuki Ishida1Emi Minaguchi2Kazuhisa Sugai3Kyohei Daigo4Yuji Iwasawa5Genki Ichihara6Kengo Nagashima7Yasunori Sato8Kazuki Sato9Yoshinori Katsumata10Institute for Integrated Sports Medicine, Keio University School of Medicine, Tokyo, JapanClinical and Translational Research Center, Keio University Hospital, Tokyo, JapanInstitute for Integrated Sports Medicine, Keio University School of Medicine, Tokyo, JapanInstitute for Integrated Sports Medicine, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanDepartment of Cardiology, Keio University School of Medicine, Tokyo, JapanClinical and Translational Research Center, Keio University Hospital, Tokyo, JapanClinical and Translational Research Center, Keio University Hospital, Tokyo, Japan; Department of Biostatistics, Keio University School of Medicine, Tokyo, JapanInstitute for Integrated Sports Medicine, Keio University School of Medicine, Tokyo, JapanInstitute for Integrated Sports Medicine, Keio University School of Medicine, Tokyo, Japan; Department of Cardiology, Keio University School of Medicine, Tokyo, Japan; Corresponding author. Institute for Integrated Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.The effect of single-nanosized oxygen nanobubble water (NBO2W), an oxygen-enriched beverage, on exercise performance remains unclear. This double-blind, randomized, placebo-controlled crossover trial investigated whether NBO2W intake influences lactate threshold (LT), a key indicator of aerobic exercise performance. The participants (n = 20; age, 47.4 ± 5.0 years; female, 12) consumed either NBO2W or placebo water (PW) immediately followed by an incremental exercise test on an ergometer for symptom-limited exhaustion. During the test, the heart rate, respiratory gas analysis (VO2 and VCO2), tissue oxygen index (TOI), and blood lactate level were continuously monitored. The first administered beverage was randomly selected, with both participants and investigators blinded to the beverages. A washout period of minimum seven days was implemented between the two tests. The mean peak VO2 was 26.13 ± 5.05 mL/min/kg. Eighteen (90 %) participants exercised less than four times per week, indicating limited exercise habits. The average washout period was 9.7 ± 5.0 days. The work rate (WR) at LT was significantly higher after NBO2W intake than after PW intake (mean difference = 3.33 W, p = 0.03). The WR was not significantly different at peak VO2 or at the onset of blood lactate accumulation. Furthermore, NBO2W intake significantly increased the lactate levels 5 min post-exercise (mean difference = 0.88, p = 0.01) and decreased the TOI (mean difference = −3.8, p = 0.01). Short-term NBO2W consumption in three doses, including that on the day before exercise, did not affect the peak exercise capacity, but improved the WR at LT.http://www.sciencedirect.com/science/article/pii/S2665944125000215Single-nanosized oxygen nanobubble waterExercise toleranceLactate thresholdWork rateTissue oxygen index
spellingShingle Yuki Muramoto
Noriyuki Ishida
Emi Minaguchi
Kazuhisa Sugai
Kyohei Daigo
Yuji Iwasawa
Genki Ichihara
Kengo Nagashima
Yasunori Sato
Kazuki Sato
Yoshinori Katsumata
Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
Current Research in Physiology
Single-nanosized oxygen nanobubble water
Exercise tolerance
Lactate threshold
Work rate
Tissue oxygen index
title Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
title_full Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
title_fullStr Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
title_full_unstemmed Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
title_short Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial
title_sort positive effects of single nanosized oxygen nanobubble water on exercise tolerance a randomized crossover trial
topic Single-nanosized oxygen nanobubble water
Exercise tolerance
Lactate threshold
Work rate
Tissue oxygen index
url http://www.sciencedirect.com/science/article/pii/S2665944125000215
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